Picro Sirius Red Stain  天狼星红染色液

产品标签

Picro Sirius Red 天狼星红，Connective Tissue Stain结 缔组织染色，collagen I 胶原I纤维，collagen III 胶原III纤维；CAS：2610-10-8；

产品信息

产品描述

天狼星红染色（Picro Sirius Red Stain）是一种简单和灵敏的方法用于鉴定组织切片中的胶原纤维网络，通过形态成像分析来定性或定量鉴别退行性病变和遗传性或后天性疾病内胶原网络的异常变化。天狼星红是一种强且线性的阴离子染料，包含6个磺酸基，能够与阳离子胶原纤维牢牢结合。偏振光检测下，胶原纤维有正的单轴双折射光属性，当与天狼星红染料结合后，胶原的天然双折射属性得以增强，从而能区分两型胶原纤维。

本品为即用型的天狼星红染色液（Picro Sirius Red Stain），操作简单快捷，用于胶原纤维的染色。在偏振光下，通过对胶原纤维的双折射光来区分I型胶原和III型胶原。也能在光学显微镜下观察胶原染色。

保存与运输方法

保存：4℃避光保存，1年有效。

运输：室温运输。

注意事项

1. 为使偏振光镜下显色清晰，切片厚度以6~7μm为宜。

2. 若需要做细胞核复染，可选择我司的天狼星红染色试剂盒（货号：MM1004-100ML）。

3. 为了您的安全和健康，请穿实验服并戴一次性手套操作。

4. 
   

5. 操作步骤

一、  需要自行准备的材料

1）  10%福尔马林固定液（比如：MM1519-500ML）

2）  中性树胶封片剂（比如：MM1503-100ML）3）  普通光学显微镜或偏振光显微镜

二、染色步骤

1. 组织固定于10%福尔马林固定液，常规脱水包埋。

2. 切片厚度6~7μm为宜，以按常规方法脱蜡至水。

3. 天狼星红染色液染色1h（亦可滴染）。

4. 0.5%稀乙酸快速冲洗2次。

5. 无水乙醇快速冲洗切片。

6. 常规脱水透明、中性树胶封固。

7. 普通光学显微镜或偏振光显微镜下观察。

三、染色结果

1. 普通光学显微镜观察

2. 偏振光显微镜观察

相关产品

 — —Written/Edited by V. Shallan【版权归MKBio懋康所有】

上海懋康生物科技有限公司是一家涉足于生命科学和生物技术领域研究的试剂、仪器和实验室消耗品与实验服务工作，主要从事细胞生物学、植物学、分子生物学、免疫学、生物化学、蛋白组学。生物制药与诊断试剂研发生产等领域。 本公司秉承“以人为本，以诚为信、合同守信”的经营理念。坚持"品质保障"的原则为广大客户提供优质产品。

引用文献

[1] Song, X., Shi, J., Liu, J. et al. Recombinant truncated latency-associated peptide alleviates liver fibrosis in vitro and in vivo via inhibition of TGF-β/Smad pathway. Mol Med 28, 80 (2022). https://doi.org/10.1186/s10020-022-00508-2

The liver tissue was immediately fixed in a 10% formaldehyde solution and gradient ethanol for dehydration treatment, then embedded in paraffin to make 5 μm thick tissue sections. Before pathological staining, the slices were placed in an oven at 60 ℃ for 2 h, then dewaxed with xylene and rehydrated with gradient ethanol. Tissue sections were then stained with H&E, Sirius Red (Cat.MM1004, MaoKang biotechnology Co., Ltd, Shanghai, China), Masson, and were performed to observe the structural changes and collagen fiber deposition of the liver tissues in each group by an optical microscope.

[2] Ying F, Guo J, Gao X, Huang L, Gao L, Cai J, Wang Z. Establishment of highly metastatic ovarian cancer model with omental tropism via in vivo selection. iScience. 2023 Apr 23;26(5):106719. doi: 10.1016/j.isci.2023.106719. PMID: 37197325; PMCID: PMC10183668.

Picrosirius red (MM1036, Maokang Biotech, China) was used to stain collagen in paraffin tissues.

[3] Wang Y, Li X, Deng F and Yin R (2021) Hydroxy-Safflower Yellow A Alleviates Osteoporosis in Ovariectomized Rat Model by Inhibiting Carbonic Anhydrase 2 Activity. Front. Pharmacol. 12:734539. doi: 10.3389/fphar.2021.734539

Sirius Red Staining

For Collagen fiber determination, sections were dewaxed in water, stained in Sirius red solution for 1 h, rinsed in tap water for 1 h, then nucleated by hematoxylin, routinely dehydrated, sealed with clear gum, and observed with an Olympus polarised light microscope (DM500, Leica, with polarizer installed on the aperture of the microscope). The nuclei were then stained with hematoxylin for 1 h, dehydrated, and sealed with clear gum. Two types of collagen fibers could be seen under the polarised light microscope. Type I collagen fibers were closely packed and showed strong birefringence, with yellow or red fibers. Type III collagen showed weak birefringence, with fine green fibers. It carried out the specific procedures of this experiment according to the instructions of Shanghai MKbio Biological Technology Co., LTD. Sirius red Staining Kit (cat no. MM1004). The experimental results were measured by Image-Pro Plus 6.0 (IPP 6.0), and the results were presented in the form of area proportion.

[4] Wang, Y., Zhang, S., Yang, H. et al. MicroRNA-196a-5p overexpression in Wharton’s jelly umbilical cord stem cells promotes their osteogenic differentiation and new bone formation in bone defects in the rat calvarium. Cell Tissue Res 390, 245–260 (2022). https://doi.org/10.1007/s00441-022-03673-2

For Picrosirius Red staining, the Picro Sirius Red Stain Kit (MaoKang Bio, China) was used. Briefly, cell sheet sections were deparaffinized and hydrated with distilled water. The tissue section was adequately covered in Picrosirius Red Solution.

[5] Li N, Li Z, Fang F, Zhu C, Zhang W, Lu Y, Zhang R, Si P, Bian Y, Qin Y and Jiao X (2022) Two distinct resident macrophage populations coexist in the ovary. Front. Immunol. 13:1007711. doi: 10.3389/fimmu.2022.1007711

 For collagen staining, tissue sections were also stained with Sirius red with Picro Sirius Red Stain Kit (Maokangbio). 

[6] Wang H, Zhang J, Zhang X, Zhao N, Zhou Z, Tao L, Fu L, Peng S, Chai J. Fluorofenidone ameliorates cholestasis and fibrosis by inhibiting hepatic Erk/-Egr-1 signaling and Tgfβ1/Smad pathway in mice. Biochim Biophys Acta Mol Basis Dis. 2022 Dec 1;1868(12):166556. doi: 10.1016/j.bbadis.2022.166556. Epub 2022 Sep 23. PMID: 36154893.

Five-μm sections were prepared for hematoxylin and eosin (H&E) (Solarbio, Beijing, China) and Sirius red (MaoKang Biotechnology, Shanghai, China) staining as well as… 

[7]  Ren H, Huang M, Ou L, Deng X, Wu X, Gong Q, Liu B. Autophagy inhibitor 3-methyladenine attenuates renal injury in streptozotocin-induced diabetic mice. Iran J Basic Med Sci. 2024;27(7):793-800. doi: 10.22038/IJBMS.2024.71378.15518. PMID: 38800022; PMCID: PMC11127078.

Histology and immunohistochemistry examination

The kidneys were fixed in 10% neutral formalin liquid for more than 48 hr. Kidneys were cut in half lengthwise, dehydrated in 95% ethanol overnight after ethanol gradient dehydration, then cleared with n-butanol and embedded in paraffin blocks. 4 μm slices were made with a paraffin sectioning machine. After 2 hr of baking, the slices were dewaxed and hydrated, and stained with Sirius Red (Maokang Bio, Shanghai, China) and Periodic acid–Schiff (PAS) staining solution. 

[8] Fang L, Yan Y, Xu Z, He Z, Zhou S, Jiang X, Wu F, Yuan X, Zhang T, Yu D. Tectochrysin ameliorates murine allergic airway inflammation by suppressing Th2 response and oxidative stress. Eur J Pharmacol. 2021 Jul 5;902:174100. doi: 10.1016/j.ejphar.2021.174100. Epub 2021 Apr 18. PMID: 33878335.

 Sirius red was from Maokang Biotechnology Co. (Shanghai, China).

[9]Li X, Hou R, Ding H, Gao X, Wei Z, Qi T, Fang L. Mollugin ameliorates murine allergic airway inflammation by inhibiting Th2 response and M2 macrophage activation. Eur J Pharmacol. 2023 May 5;946:175630. doi: 10.1016/j.ejphar.2023.175630. Epub 2023 Mar 4. PMID: 36871665.

Sirius red was from Maokang Biotechnology Co. (Shanghai, China).

[10]Yu, L., Liang, Y., Zhang, M. et al. Extracellular vesicle-derived circCEBPZOS attenuates postmyocardial infarction remodeling by promoting angiogenesis via the miR-1178-3p/PDPK1 axis. Commun Biol 6, 133 (2023). https://doi.org/10.1038/s42003-023-04505-x

【染色对象：小鼠心脏组织】

Histopathological changes analysis

Hematoxylin and eosin (H&E), Masson and Sirius red staining were performed to evaluate the histopathological changes in vivo44. Briefly, heart tissue was sliced into 8-µm-thickness sections and fixed with 4% paraformaldehyde at room temperature. The tissue sections were then stained with H&E Staining Kit (C0105S, Beyotime, China), Masson staining solution (R20379, YuanYe Biotech, Shanghai, China) and Sirius-Red staining (2610-10-8, Maokang Biotech, Shanghai, China), respectively. The sections were dehydrated and sealed with neutral gum and subsequently washed with running water and imaged under a microscope (Olympus, Japan). Three infarct areas were taken from each mouse from >three independent mice.

[11] Yang Y, Cai J, Yang X, Wang K, Sun K, Yang Z, Zhang L, Yang L, Gu C, Huang X, Wang Z, Zhu X. Dysregulated m6A modification promotes lipogenesis and development of non-alcoholic fatty liver disease and hepatocellular carcinoma. Mol Ther. 2022 Jun 1;30(6):2342-2353. doi: 10.1016/j.ymthe.2022.02.021. Epub 2022 Feb 19. PMID: 35192934; PMCID: PMC9171149. 

【染色对象：小鼠肝脏组织石蜡切片】

Sirus red staining was carried out on liver sections according to manufacturers’ protocols (Maokang Biotechnology, Shanghai, China).

[12] Liru Hu et al. Engineering ADSCs by manipulating YAP for lymphedema treatment in a mouse tail model. Sec. Biomedical Engineering Volume 249 - 2024 | https://doi.org/10.3389/ebm.2024.10295

And Picro-Sirius red staining was performed to analyze the collagen type I and III by using the commercial Kit (MM1036, Maokang bio, Shanghai, China) according to the manufacturer’s instructions. 

[13] Fan Y, Guo X, Tian Y, Li J, Xi H. Botulinum toxin type A inhibits the formation of hypertrophic scar through the JAK2/STAT3 pathway. Biomol Biomed. 2024 Aug 10. doi: 10.17305/bb.2024.10906. Epub ahead of print. PMID: 39132968.

Following that, cells were stained with 0.1% Sirius red (MM1004, Maokangbio, Shanghai, China) for 1 h and w ashed with tap wa ter for 1 min.

[14]Zirong Lu, Yuan Li, Chuanghong Lu, Zhongyuan Meng, Ling Bai, Feng Huang, Zhiyu Zeng. Inhibition of Endothelial–Mesenchymal Transition Mediated by Activin Receptor Type IIA Attenuates Valvular Injury Induced by Group A Streptococcus in Lewis Rats. Front. Biosci. (Landmark Ed) 2025, 30(1), 26370. https://doi.org/10.31083/FBL26370  【染色对象：大鼠心脏组织石蜡切片】

[15]Chen, Juan^∗; Chen, Xin Yi^∗; Cong, Xiao Xia^†,‡; Wang, Shen^∗; Xu, Shui Bo^†; Sun, Yu Ting^†; Zhou, Yi Ting^†,‡; Zheng, Li Ling^∗†,‡; Huang, Man^∗. CELLULAR SENESCENCE IMPLICATED IN SEPSIS-INDUCED MUSCLE WEAKNESS AND AMELIORATED WITH METFORMIN. Shock 59(4):p 646-656, April 2023. | DOI: 10.1097/SHK.0000000000002086

[16]Hou L, Guo Y, Xu S, Bai M, Cao W, Zhang Y, Jia Z, Zhang A. HNF3α Targets Nckap1l and Promotes Renal Fibrosis Following Ischemia-Reperfusion Injury. Adv Sci (Weinh). 2025 Mar 17:e2410764. doi: 10.1002/advs.202410764. Epub ahead of print. PMID: 40091743. 【染色对象：小鼠肾脏组织切片】

Thus, 5-µm slices were subjected to deparaffinization and rehydration, staining with Sirius Red solution (Maokang Biotechnology, Shanghai, China) at room temperature for 1 h, and then rinsing, dehydration, and mounting.

[17]Zhang C, Teng Y, Bai X, Tang M, Stewart W, Chen JJ, Xu X, Zhang XQ. Prevent and Reverse Metabolic Dysfunction-Associated Steatohepatitis and Hepatic Fibrosis via mRNA-Mediated Liver-Specific Antibody Therapy. ACS Nano. 2024 Dec 17;18(50):34375-34390. doi: 10.1021/acsnano.4c13404. Epub 2024 Dec 5. PMID: 39639502.