| 目录号 | MM1004-100ML | 售价 | 550.00元 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 规格 | 2×50ml | 运输温度 | 室温 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 其他名称 | PicroSirius Red Stain Kit 天狼猩红染色试剂盒； | 保存温度 | 4℃避光保存 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| CAS号 | N/A | 有效期 | 1年 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 应用 | 胶原染色 | 订购数量 |
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产品简介: Picro Sirius Red Stain Kit 天狼星红染色试剂盒
产品标签 Picro Sirius Red 天狼星红，Connective Tissue Stain 结缔组织染色，collagen I 胶原I纤维，collagen III 胶原III纤维；
产品信息【现货供应】
「注意」：不需要做细胞核复染，可单独订购天狼星红染色液，信息如下：
产品描述 天狼星红染色（Picro Sirius Red Stain）是一种简单和灵敏的方法用于鉴定组织切片中的胶原纤维网络，通过形态成像分析来定性或定量鉴别退行性病变和遗传性或后天性疾病内胶原网络的异常变化。天狼星红是一种强且线性的阴离子染料，包含6个磺酸基，能够与阳离子胶原纤维牢牢结合。偏振光检测下，胶原纤维有正的单轴双折射光属性，当与天狼星红染料结合后，胶原的天然双折射属性得以增强，从而能区分两型胶原纤维。
本天狼星红染色试剂盒（Picro Sirius Red Stain Kit）主要由天狼星红染色液、Mayer苏木素染色液组成，用于胶原纤维I和胶原纤维III的染色。在偏振光下，通过对胶原纤维的双折射光来区分I型胶原和III型胶原。也能在光学显微镜下观察胶原染色。本染色液稳定，不易褪色。且操作简单。
产品组成
保存与运输方法 保存：4℃避光保存，1年有效。 运输：室温运输。
注意事项 1） 为使偏振光镜下显色清晰，切片厚度以6~7μm为宜。 2） 复染细胞核宜用Mayer’s苏木素染色液，其不影响I型胶原和III型胶原的数量和双折射强度。 3） 为了您的安全和健康，请穿实验服并戴一次性手套操作。
操作步骤 一、 需要自行准备的材料 1） 10%福尔马林固定液（比如：MM1519-500ML） 2） 中性树胶封片剂（比如：MM1503-100ML） 3） 普通光学显微镜或偏振光显微镜
二、染色步骤 1） 组织固定于10%福尔马林固定液，常规脱水包埋。 2） 切片厚度6~7μm为宜，以按常规方法脱蜡至水。 3） 天狼星红染色液滴染1h。 4） 流水稍微冲洗，去掉切片表面染色液。 5） Mayer’s苏木素染色液染细胞核8~10min。 6） 流水冲洗10min。 7） 常规脱水透明、中性树胶封固。 8） 普通光学显微镜或偏振光显微镜下观察。
三、染色结果 1） 普通光学显微镜观察
2） 偏振光显微镜观察
相关产品
引用文献
[1] Song, X., Shi, J., Liu, J. et al. Recombinant truncated latency-associated peptide alleviates liver fibrosis in vitro and in vivo via inhibition of TGF-β/Smad pathway. Mol Med 28, 80 (2022). https://doi.org/10.1186/s10020-022-00508-2
The liver tissue was immediately fixed in a 10% formaldehyde solution and gradient ethanol for dehydration treatment, then embedded in paraffin to make 5 μm thick tissue sections. Before pathological staining, the slices were placed in an oven at 60 ℃ for 2 h, then dewaxed with xylene and rehydrated with gradient ethanol. Tissue sections were then stained with H&E, Sirius Red (Cat.MM1004, MaoKang biotechnology Co., Ltd, Shanghai, China), Masson, and were performed to observe the structural changes and collagen fiber deposition of the liver tissues in each group by an optical microscope.
[2] Wang Y, Li X, Deng F and Yin R (2021) Hydroxy-Safflower Yellow A Alleviates Osteoporosis in Ovariectomized Rat Model by Inhibiting Carbonic Anhydrase 2 Activity. Front. Pharmacol. 12:734539. doi: 10.3389/fphar.2021.734539 Sirius Red StainingFor Collagen fiber determination, sections were dewaxed in water, stained in Sirius red solution for 1 h, rinsed in tap water for 1 h, then nucleated by hematoxylin, routinely dehydrated, sealed with clear gum, and observed with an Olympus polarised light microscope (DM500, Leica, with polarizer installed on the aperture of the microscope). The nuclei were then stained with hematoxylin for 1 h, dehydrated, and sealed with clear gum. Two types of collagen fibers could be seen under the polarised light microscope. Type I collagen fibers were closely packed and showed strong birefringence, with yellow or red fibers. Type III collagen showed weak birefringence, with fine green fibers. It carried out the specific procedures of this experiment according to the instructions of Shanghai MKbio Biological Technology Co., LTD. Sirius red Staining Kit (cat no. MM1004). The experimental results were measured by Image-Pro Plus 6.0 (IPP 6.0), and the results were presented in the form of area proportion.
— —Written/Edited by V. Shallan【版权归MKBio懋康所有】
上海懋康生物科技有限公司是一家涉足于生命科学和生物技术领域研究的试剂、仪器和实验室消耗品与实验服务工作，主要从事细胞生物学、植物学、分子生物学、免疫学、生物化学、蛋白组学。生物制药与诊断试剂研发生产等领域。 本公司秉承“以人为本，以诚为信、合同守信”的经营理念。坚持"品质保障"的原则为广大客户提供优质产品。
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