Stemfactor™ Activin A, Human Recombinant 重组人激活素A

产品关键词：

naïve stem cells 初始干细胞；neural progenitor cells 神经祖细胞；WH-4-023；CHIR99021；Stemfactor™ LIF；

产品描述：

Stemfactor™ Activin A是一种结晶级别的重组蛋白制备物，提供非常高的纯度。激活素A（Activin A）具有双重功能，不仅能维持干细胞潜能也能促进分化。成熟的重组人激活素A是2条116个氨基酸残基组成的βA亚基通过二硫键连接的二聚体，分子量约25kDa。

Stemgent公司提供支持初始潜能干细胞应用需要的5i/L/A培养基添加剂（5i/L/A media supplment）所有成分，如下：

产品特性

1. 来源：E.Coli

2. 配方：Lyophilized from 20% acetonitrile

3. 氨基酸序列：GLECDGKVNI CCKKQFFVSF KDIGWNDWII APSGYHANYC EGECPSHIAG TSGSSLSFHS TVINHYRMRG HSPFANLKSC CVPTKLRPMS MLYYDDGQNI IKKDIQNMIV EECGCS

4. Uniprot序列号：P08476, residues 311-426

5. 纯度：＞98%（SDS-PAGE）

6. 内毒素水平：Less than 1.0 EU/μg of Activin A as determined by the LAL method.

7. 生物活性：The ED50 is less than 6 ng/ml as determined by its ability to inhibit the proliferation of mouse MPC-11 cells.

8. 重溶：Centrifuge briefly and reconstitute Activin A in 10 mM HCl before dilution in cell culture medium. Avoid freeze-thaw cycles as it can result in loss of activity.

9. 保存和稳定性：Activin A is shipped at room temperature. Lyophilized Activin A is stable for up to 6 months from date of receipt when stored at -20°C to -80°C.  Reconstituted Activin A, at concentrations greater than 0.1 mg/ml, is stable for up to 3 months when stored at -20°C and for 1 year when stored at -80°C

订购信息：原装正品，欢迎订购，联系电话：021-54736159，QQ：2971634497。

引用文献：

• Villa-Diaz LG; Kim JK; Laperle A; Palecek SP; Kresbach PH. "Inhibition of focal adhesion kinase signaling by integrin a6b1 supports human stem cell self-renewal." Stem Cells doi: 10.1002/stem.2349 (2016)

• Zhu S; Russ HA; Wang X; Zhang M; Ma T; Xu T; Tang S; Hebrok M; Deng S. "Human pancreatic beta-like cells converted from fibroblasts." Nature Commun 7:10080 (2016)

• Saxena S; Ronn RE; Guibentif C; Moragheba R; Woods N-B. "Cyclic AMP signaling through Epac axis modulates human hemogenic endothelium and enhances hematopoeitic cell generation." Stem Cell Rep doi:10.1016/j.stemcr.2016.03.006 (2016)

• Mora-Castilla S; To C; Vaezeslami S; Morey R; Srinivasan S; Dumdie JN; Cook-Andersen H; Jenkins J; Laurent LC. "Miniaturization technologies for efficient single-cell library preparation for next-generation sequencing." J Lab Automation 2016:1 (2016)

• Qian X. "Defining the mechanism by which synthetic polymer surfaces support human pluripotent stem cell self-renewal." PhD Thesis, University of Michigan (2015)

• Villa-Diaz LG; Kim JK; Lahann J; Kresbach PH. "Derivation and long-term culture of transfene-free human induced pluripotent stem cells on synthetic substates." Stem Cells Trans Med 3:1410 (2014)

• Bukong, T.N., Lo, T., Szabo, G., and Dolganiuc, A. (2012) Novel developmental biology-based protocol of embryonic stem cell differentiation to morphologically sound and functional yet immature hepatocytes. Liver Int. doi: 10.1111/j.1478-3231.2011.02743.x. [Epub ahead of print]

• Kunisada, Y., Tsubooka-Yamazoe, N., Shoji, M., and Hosoya, M. (2012) Small molecules induce efficient differentiation into insulin-producing cells from human induced pluripotent stem cells. Stem Cell Res 8: 274-284.

• McCracken, K.W., Howell, J.C., Wells, J.M., and Spence, J.R. (2011) Generating human intestinal tissue from pluripotent stem cells in vitro. Nat Protoc 6: 1920-1928.

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• 订购信息：

  电话：021-54736159

  QQ：2971634497

  邮箱：sales@maokangbio.com

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   — —Written/Edited by V. Shallan【版权归MKBio懋康所有】

   

   

  上海懋康生物科技有限公司是一家涉足于生命科学和生物技术领域研究的试剂、仪器和实验室消耗品与实验服务工作，主要从事细胞生物学、植物学、分子生物学、免疫学、生物化学、蛋白组学。生物制药与诊断试剂研发生产等领域。 本公司秉承“以人为本，以诚为信、合同守信”的经营理念。坚持"品质保障"的原则为广大客户提供优质产品。

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