FD NeuroTechnologies, Inc.；Golgi-Cox impregnation 浸染法；Neuronal apoptosis 神经元凋亡；Fluoro-Jade B；In situ DNA nick-end labeling (TUNEL) 原位缺口末端标记法；
产品描述：
FD NeuroTechnologies, Inc（USA）公司生产的FD神经元凋亡检测试剂盒（FD NeuroApop™ Kit）特别研发用于中枢神经系统组织切片中的神经元凋亡检测，基于原位DNA缺口末端标记技术（TUNEL法）。该技术使用末端脱氧核糖核酸转移酶（TdT）催化生物素偶联的脱氧尿嘧啶插入DNA片段自由的3’-OH末端，其被认为是凋亡最典型特征之一。之后插入DNA的生物素使用亲和素-生物素复合物（ABC）方法得以信号扩大，并在光学显微镜下进行可视化鉴定。
FD神经元凋亡检测试剂盒（FD NeuroApop™ Kit）提供的试剂和步骤都经过优化以能在特异性和灵敏度上高效检测凋亡神经元，且维持极低背景。此试剂盒适用于冰冻和石蜡切片，以及培养细胞。本试剂盒整个操作流程约需4h。
试剂盒组分（PK201）：
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|
|
PK201（For 35 sections） |
Storage |
|
1 |
Digestive Enzyme 消化酶 |
2 ml x 4 |
-20℃ |
|
2 |
Reaction Solution A 溶液A |
2 ml x 2 |
-20℃ |
|
3 |
Reaction Solution B 溶液B |
85 µl |
-20℃ |
|
4 |
Reaction Solution C 溶液C |
60 µl |
-20℃ |
|
5 |
Chromogen Solution 显色液 |
20 ml |
-20℃ |
|
6 |
Equilibration Buffer 平衡缓冲液 |
20 ml |
+4℃ |
|
7 |
Detection Reagent 检测试剂 |
5 ml |
+4℃ |
|
8 |
10x Phosphate-Buffered Saline 磷酸盐 |
250 ml x 2 |
+4℃ |
应用实例：
图1. Detection of apoptotic neurons with FD NeuroApop™ Kit. Paraffin section (10µm) cut from a dorsal ganglion of a mouse embryo (E17). The section was processed with FD NeuroApop™ kit and counterstained with methyl green. Sections courtesy of Drs. Michael Vogel and Lisa Qiu.
图2. Detection of apoptotic neurons with FD NeuroApop™ Kit. Paraffin section (10µm) cut from the inferior colliculus of a mouse embryo (E17). The section was processed with FD NeuroApop™ kit and counterstained with methyl green. Sections courtesy of Drs. Michael Vogel and Lisa Qiu.
图3. Detection of apoptotic neurons with FD NeuroApop™ Kit. 20 µm cryostat section was cut from the rat striatum of a stroke model. The section was processed for detecting neuronal apoptosis with FD NeuroApop™ Kit and then counterstained with FD methyl green.
订购信息：原装正品，欢迎来电咨询。电话：021-54736159，18121428569。QQ：3308240863，2971634497。网址：www.maokangbio.com
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货号 |
名称 |
规格 |
价格（元） |
|
PK201 |
FD NeuroApop™ Kit FD神经元凋亡检测试剂盒 |
For 35 sections |
7176 |
|
PK101 |
FD Apop™ Kit Tunel凋亡检测试剂盒 |
For 50 sections |
6118 |
几十篇使用文献的数据支持，是值得信赖的科研辅助精品。详见操作手册。
PK201-FD NeuroApop™ Kit User Mannual
PK101-FD Apop™ Kit User Manual
技术文献：
[1] Gavrieli Y, Sherman Y, and Ben-Sasson SA. (1992) Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J. Cell Biol. 119:493-501.
[2] Wyllie AH. (1980) Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation. Nature. 284:555-6.
[3] Arends MJ, Morris RG, and Wyllie AH. (1990) Apoptosis: the role of the endonuclease. Amer. J. Pathol 136:593-608.
[4] Hsu SM, Raine L, and Fanger H. (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J. Histochem. Cytochem. 29:577-80.
— —Written/Edited by V. Shallan【版权归MKBio懋康所有】
上海懋康生物科技有限公司是一家涉足于生命科学和生物技术领域研究的试剂、仪器和实验室消耗品与实验服务工作，主要从事细胞生物学、植物学、分子生物学、免疫学、生物化学、蛋白组学。生物制药与诊断试剂研发生产等领域。 本公司秉承“以人为本，以诚为信、合同守信”的经营理念。坚持"品质保障"的原则为广大客户提供优质产品。
