EAE（实验性自身免疫性脑脊髓炎）模型建立：MOG (35-55) 髓鞘少突胶质细胞糖蛋白（35-55）

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时间：2016-09-27 作者：懋康生物文章来源：懋康原创

产品关键词：

MOG (35-55) 髓鞘少突胶质细胞糖蛋白（35-55）；中枢神经系统（CNS）；Multiple Sclerosis (MS) 多发性硬化症；实验性自身免疫性脑脊髓炎（EAE）；PLP (178-191)；MBP (87-99)；CAS：149635-73-4；

背景描述：

实验性自身免疫性脑脊髓炎（Experimental Autoimmune Encephalomyelitis，EAE）是最常用的实验性动物模型用来研究人炎症脱髓鞘病，多发性硬化病（Multiple sclerosis，MS）。

EAE是一系列免疫病理和神经病理机制交叉作用引发的综合性状况，导致机体出现近似MS的关键性病特征：炎症反应，脱髓鞘，轴突丧失和胶质增生。就减少炎症的反调控机理和髓鞘再生也发生EAE模型中，因此，该模型也可用来研究这些过程。另外，EAE通常用作细胞介导的器官特异性的自身免疫疾病的模型。

EAE具有复杂的神经药理学特征，许多目前已用或即将使用的MS治疗药物得以开发、测试或验证，基于EAE研究。

目前主要由三种髓鞘蛋白（或蛋白多肽）用于诱发EAE模型的研究：髓鞘少突胶质细胞糖蛋白（MOG），髓鞘碱性蛋白（MBP）和髓鞘蛋白脂质蛋白（PLP）。

※MBP是最早用来EAE研究的髓鞘抗原，多以诱发急性EAE（Acute EAE）模型。

※MOG是目前常用建立EAE模型的髓鞘抗原，多以诱发慢性EAE（Chronic EAE）模型。

※PLP也是常用建立EAE模型的髓鞘抗原，模型呈现缓解-复发的特点，多用来建立复发-缓解型（Relapse remitting EAE，RR-EAE）模型。

MOG (35-55)基本描述：

MOG，英文全名Myelin oligodendrocyte glycoprotein，中文名髓鞘少突胶质细胞糖蛋白，髓鞘的一种微量成分，属于免疫球蛋白超家族成员之一。也是特定表达于中枢神经系统（CNS）的自身抗原，诱导多发性硬化症的原发性脱髓鞘特征。

MOG (35-55)是髓鞘少突胶质细胞糖蛋白的免疫优势表位，能够诱导强烈的T细胞和B细胞应激反应，具高度致脑炎性，能够诱导啮齿类动物的实验性自身免疫性脑脊髓炎（EAE）模型。EAE是最普遍的MS动物模型，具有MS许多的临床和病理生理学特征。单次注射MOG (35-55)能够产生一种复发-缓解型神经性疾病，表现出大量斑块状脱髓鞘病症。1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）诱导的多巴胺神经元损伤模型，免疫接种MOG (35-55)能抑制该神经元的自发再生功能。

MOG (35-55)基本特性：

我司提供两种类型的MOG（35-55），一种是大小鼠MOG（35-55）（货号：MP5413-1MG），一种是人MOG（35-55）（货号：MP5414-1MG），都可诱导建立EAE模型。两者诱导模型方式的差异可见

文献描述：Oliver AR.,Rat and human myelin oligodendrocyte glycoproteins induce experimental autoimmune encephalomyelitis by different mechanisms in C57BL/6 mice. J Immunol. 2003 Jul 1; 171(1):462-8.

名称	大小鼠MOG（35-55）	人MOG（35-55）
货号	MP5413-1MG	MP5414-1MG
CAS NO：	149635-73-4 [net]	163158-19-8
分子式	C118H177N35O29S	C120H179N35O28S
分子量	2581.97	2591.99
纯度	≥98%（HPLC）	≥98%（HPLC）
单字母序列	MEVGWYRSPFSRVVHLYRNGK	MEVGWYRPPFSRVVHLYRNGK

EAE模型（MOG）诱导方法：

A）常用动物品系（strain）：C57BL/6 mice（有时称为B6）

B）诱导方法：将MOG（35-55）乳化入弗氏完全佐剂佐剂CFA后皮下注射免疫动物。还可添加腹腔注射百日咳毒素，其能促使免疫细胞穿透血脑屏障。常用注射计量是200μg MOG/小鼠。

【详细诱导步骤可参考资料Stefan Bittner et al., Myelin Oligodendrocyte Glycoprotein (MOG35-55) Induced Experimental Autoimmune Encephalomyelitis (EAE) in C57BL/6 Mice.或者咨询我司021-54736159】。

C）诱导结果：鼠尾肌肉紧张度完全丧失和后肢功能欠缺，约在MOG诱导后第12天发生。加入药物泼尼松龙（prednisone）可预防EAE发生，实验研究中可用作阳性对照【见下图】。

订购信息：更大包装或产品技术问题，请来电/在线咨询，电话：021-54736159 。网站：www.maokangbio.com。

货号	产品名称	规格
MP5413	MOG (35-55), Mouse, Rat大小鼠髓鞘少突胶质细胞糖蛋白	1mg/5mg/10mg/25mg/100mg
MP5414	MOG (35-55), Human人髓鞘少突胶质细胞糖蛋白	5mg/10m/25mg

【注】：可以大包装供货；亦可根据需求提供相应的包装方式。

EAE模型关联试剂：

货号	产品名称	规格
Chondrex 7001	弗氏完全佐剂（CFA，4mg/ml）	5ml
Chondrex 7023	弗氏完全佐剂（CFA，5mg/ml）	5ml
Chondrex 7002	弗氏不完全佐剂（IFA）	5ml
List 180	百日咳毒素Pertussis Toxin（PTX lyophilized in buffer）	50μg
List 181	百日咳毒素Pertussis Toxin（PTX lyophilized in water）	50μg
MP5415-1MG	PLP (139-151)髓鞘蛋白脂质蛋白（139-151）	1mg
MP5416-1MG	PLP (178-191)髓鞘蛋白脂质蛋白（178-191）	1mg

引用示例：【来自文献，EAE构建】

EAE模型建立
文献编号	动物品系	实验方法
PMID: 39936997	C57BL/6J Mice	mice were injected subcutaneously into axilla and groin with a total of200 µg of MOG35–55 peptide (MEVGWYRSPFSRV VHLYRNGK)suspended in complete Freund’s adjuvant (CFA). On the same day, 1–2 h after MOG/CFA or CFA-only injection, and on the next day, both EAE and control mice were injected intraperitoneally with pertussis toxin (PTX). In total,200 ng PTX was used for each injection, as previously described.
PMID: 39875919	9-week-old C57BL/6J Mice	mice were immunized on day 0 subcutaneously at two sites over the lateral abdomen with an emulsion ofMOG35 − 55 (200µg) in complete Freund’sadjuvant(CFA)containing 400 µg of Mycobacterium tuberculosis H37RA. Pertussis toxin (250 ng; List Biological Labs, Campbell, CA, USA) was intraperitoneally injected on days 0 and 2.
PMID:39480507	10- to 12-week-old CD45.1 mice	mice were injected s.c. into both flanks of the back regions with 300 μg per mouse of MOG35–55 peptide in CFA containing 5 mg/mL heat-killed Mycobacterium tuberculosis H37Ra. Pertussis toxin (200 ng per mouse; List Biological Laboratories) was also administered to mice in PBS on the day of immunization and 48 hours later.
PMID:39145452	Six- to 8-week-old Vegfb WT and cKO female mice	EAE was induced by subcutaneous immunization with MOG35–55 peptides (200 μg/mouse)emulsified in complete Freund’s adjuvant (catalog F5881), followed by administration ofpertussis toxin (200 ng/mouse, PTX, List Biological Laboratories Inc.) on days 0 and 2
PMID:38834505	C57BL/6J mice (male, 8–10 weeks old)	EAE was induced by subcutaneous injection (into the hind flank) of1 mg/mL of myelin oligodendrocyte glycoprotein 35–55 peptide emulsified in complete Freund’s adjuvant and supplemented with 5 mg/mL Mycobacterium tuberculosis H37Ra.Pertussis toxin (500 ng; List Biological Laboratories, USA) was intraperitoneally injected on days 0 and 2 postimmunization (PI).
PMID:38491084	WT C57BL/6 mice	mice were subcutaneously immunized with200 μg of murine myelin oligodendrocyte glycoprotein (MOG)35–55 peptide in complete Freund’s adjuvant (7001, Chondrex Inc.,. Each mouse was intraperitoneally injected with400 ng of pertussis toxin (Catalog Number: 181, List Biological Laboratories) twice at 0 h and 48 hpostimmunization.
PMID:38323310	Rag2-/- mice	mice were subcutaneously injected with a200 μL emulsion that contains 1 mg/ml MOG35-55 peptide (MEVGWYRSPFSRVVHLYRNGK), 2.5 mg/ml Mycobacteria tuberculosis H37Ra (B.D.), and 100 μL Freund’s adjuvant (Sigma). On the day of immunization and two days after immunization, mice were intraperitoneally injected with200 ng of pertussis toxin (List Biological Laboratories Inc.).
PMID:37850588	C57Bl/6J mice SJL/J mice	Complete Freud’s Adjuvant with 4mg/mL crushed, deactivated mycobacterium tuberculosis and1mg/mL myelin peptide [MOG 35-55 (C57Bl/6) or PLP139-151 (SJL/J)] were emulsified and injected subcutaneously above shoulders and hips of anesthetized mice, 50μL per injection site for a total of 200μL emulsion. For studies using C57Bl/6J mice, pertussis toxin (List Labs, 50μg/mL) in 200μL PBS was diluted to200-400ng depending on lot potency and injected intraperitoneal (i.p.) on the day of immunization (day 0) and two days post-immunization (day 2).
PMID:37663126	SJL/J mice	mice were immunized subcutaneously in two sites (left and right flank) with150 μg of PLP139-151emulsified in complete Freund’s adjuvant (CFA) containing 200 μg Mycobacterium Tuberculosis (Difco Laboratories, Detroit, MI). Mice received200 ng pertussis toxin (PT, List Biological Laboratories Inc., Campbell, CA) in 0.2 ml PBS (Lonza, Walkersville, MD) intraperitoneally (ip) at the time of immunization and 48 h later. Control mice were immunized with CFA followed by PT.
PMID:37629117	SJL/J mice or A.SW mice, C57BL/6 mice	We induced EAE by subcutaneous (s.c.) sensitization with 100 nmol/mouse of modified PLP139–151 peptide (VSLGKWLGHPDKF) in SJL/J mice, MOG92–106 peptide (DEGGYTCFFRDHSYQ) in SJL/J mice or A.SW mice, orMOG35–55 (MEVGWYRSPFSRVVHLYRNGK) peptide in C57BL/6 mice, in which myelin peptides were emulsified in CFA composed of incomplete Freund’s adjuvant and Mycobacterium tuberculosis H37 Ra.The final concentration of M. tuberculosis in the myelin peptide/CFA emulsion was2 mg/mL (400 μg/mouse). In MOG92–106-sensitized SJL/J mice, we injected intraperitoneally (i.p.) with 5 mg of curdlan (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) in 200 μL of phosphate-buffered saline (PBS) one day before MOG sensitization. To induce chronic MOG35–55-induced EAE, we injected C57BL/6 mice twice with MOG35–55 s.c. on days 0 and 19 and300 ng of pertussis toxin (List Biological Laboratories, Campbell, CA, USA) i.p. on days 0 and 2.

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