目录号 | MX4107-1MG | 售价 | 3380.00元 | ||||||||||||||||||||||||||||||||||
规格 | 1mg | 运输温度 | 冰袋 | ||||||||||||||||||||||||||||||||||
其他名称 | 5-chloromethylfluorescein diacetate | 保存温度 | -20℃避光干燥保存 | ||||||||||||||||||||||||||||||||||
CAS号 | 136832-63-8 | 有效期 | 2年 | ||||||||||||||||||||||||||||||||||
应用 | 活细胞示踪探针 | 订购数量 |
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产品简介: Cell-Tracker Green CMFDA 活细胞示踪探针(绿色)
产品关键词: Cell-Tracker Green CMFDA;Cell-Tracker Red CMTPX;Cell movement 细胞运动;Cell location 细胞定位;Cell Tracker dye 细胞示踪探针;CAS NO:136832-63-8;
订购信息:
产品描述
Cell-Tracker荧光探针是用来监测细胞运动、定位、增殖、迁移、趋化和侵袭的优秀工具。
Cell-Tracker Green CMFDA,英文全名:5-chloromethylfluorescein diacetate,能自由穿透细胞膜进入细胞,在胞内转化生成不具细胞膜渗透性的反应产物。该产物几次传代都能良好的保留在活细胞。在细胞群内,染料只会转移到子代细胞,不会转移到邻近细胞。
Cell-Tracker Red CMTPX特地设计使其至少72h(典型有3~6代)能展示荧光,此染料表现出理想的示踪特征:稳定、工作浓度下无毒性、良好保留在细胞,且在生理pH下呈明亮荧光。另外,Cell-Tracker Red CMTPX的激发和发射光谱(492/517nm)与红色荧光蛋白RFP很好的分开,适用于多重标记(见附表1. Cell-Tracker荧光探针的光谱特征)。
产品特性 1) CAS NO.:136832-63-8 2) 同义名:CellTracker™ Green CMFDA; Spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one, 3',6'-bis(acetyloxy)-5-(chloromethyl)-; 3) 分子式:C25H17ClO7 4) 分子量:464.86 g/mol 5) 外观:无色固体 6) 溶解性:溶于DMSO 7) 化学结构式:
保存与运输方法 保存:-20℃避光干燥保存,至少2年有效。 运输:冰袋运输。
注意事项
应用示例 文献1)Nasir NAM et al.Fluorescent cell tracer dye permits real‐time assessment of re‐epithelialization in a serum‐free ex vivo human skin wound assay. Wound Repair Regen. 2019 Jan;27(1):126-133.Method (Cell tracker dye uptake within human ex vivo wounds in situ):Four microliters of 25 μM CMFDA (or CMTPX) was added to the wound surface for 30 minutes, then washed dropwise with phosphate buffered saline.For cell viability studies, 1 μg/mL of propidium iodide (PI) was cotreated alongside CMFDA. Planimetric imaging was conducted using a Leica M205 FA upright Stereomicroscope using a 5x/0.50 pLANapo LWD objective at the equivalent of 64x magnification and captured using a DFC 565FX camera through LAS AF v3.1.0.8587 software. Specific band‐pass filter set for green fluorescent protein was used.
Fig. Epithelial repair of human ex vivo wounds progresses via an extending shield mechanism. Human ex vivo wounds were cultured in the presence of the fluorescent dyes. CMFDA and PI cotreatment at time of injury (D0) identifies live (CMFDA; green) and dead (PI; red) cells around the injury site (A), and these wounds traced to day 1 indicate PI‐labeled cells are no longer present (B). |
货号 |
名称 |
规格 |
MX4107-1MG |
Cell-Tracker Green CMFDA 活细胞示踪探针(绿色) |
1mg |
MX4108-100UG |
Cell-Tracker Orange CMTMR 活细胞示踪探针(橙色) |
2×50μg |
MX4109-100UG |
Cell-Tracker Red CMTPX 活细胞示踪探针(红色) |
2×50μg |
MX4101-1G |
Fluorescein diacetate (FDA) 二乙酸荧光素 |
1g |
MX4205-10MG |
Propidium Iodide 碘化丙啶(粉末) |
10mg |
— —Written/Edited by V. Shallan【版权归MKBio懋康所有】
上海懋康生物科技有限公司是一家涉足于生命科学和生物技术领域研究的试剂、仪器和实验室消耗品与实验服务工作,主要从事细胞生物学、植物学、分子生物学、免疫学、生物化学、蛋白组学。生物制药与诊断试剂研发生产等领域。 本公司秉承“以人为本,以诚为信、合同守信”的经营理念。坚持"品质保障"的原则为广大客户提供优质产品。