DiBAC4(5) 膜电位荧光探针

温馨提示：见我司懋康生物（MKBio）整理的膜电位荧光探针（Probes for Membrane Potential）产品专题。

搜索关键词：

DiBAC4(5)；DiSBAC2(3)；DiBAC4(3)；Oxonol V；Slow-response probes 慢速响应探针；CAS：63560-89-4；

订购信息：

产品描述

慢速响应探针（Slow-response probes）包括阳离子碳花菁、罗丹明和阴离子氧鎓醇，都适用于检测非兴奋细胞因呼吸活性、离子通道渗透性、药物结合和其他因素引起的平均膜电位变化。关于更多慢速响应探针的信息，见懋康生物官网整理的膜电位荧光探针产品专题。

DiBAC4(5)(Bis-(1,3-dibutylbarbituric acid)pentamethine oxonol)是带阴离子的慢速响应电位敏感探针，在pH4.5~7.5的范围内表现出非pH敏感的荧光特征（最大激发和发射波长分别为590nm和616nm）。能够进入去极化细胞，之后结合到胞内蛋白或膜上。一旦结合，DiBAC4(5)的荧光特性加强，往红光区迁移。增加的细胞去极化引起增多的染料内流和增高的荧光信号。DiBAC4(5)的负电荷阻止其进入线粒体，因此，适用于细胞膜电位测定。

产品特性

1. CAS NO：63560-89-4

2. 同义名：Bis-(1,3-dibutylbarbituric acid)pentamethine oxonol

3. 分子式：C29H42N4O6 

4. 分子量：542.67g/mol

5. Ex/Em：590/616 nm

6. 溶解性：溶于DMSO

7. 化学结构式：

8. 
   

9. 保存与运输方法

保存：-20℃干燥避光保存，有效期至少一年。

运输：冰袋运输。

应用示例：

文献来源：Zhang Y et al. A Membrane Potential- and Calpain-Dependent Reversal of Caspase-1 Inhibition Regulates Canonical NLRP3 Inflammasome.Cell Rep. 2018 Aug 28;24(9):2356-2369.e5.

实验方法（膜电位染色MK）：DiBAC4(5) is a translational MP dyes that redistributes within the cell membrane as MP changes. MP depolarization results in an increase in fluorescence intensity of cells at Ex/Em = 590/616nm. Conversely, hyperpolarization is indicated by a decrease in fluorescence. PMA-differentiated THP-1 cells were cultured in 35mm imaging dish with a concentration of 4x105cells/ml. Cells were stained in 200 nM DiBAC4(5)in 5mM K+ buffer at 37°C for 30 min. Then the staining buffer was removed without washing and crystals (silica, Al(OH)3, CPPD) or inhibitors (glyburide or CFTRinh-172) in fresh 5mM K+buffer containing 200nM DiBAC4(5) was added to the cells. For high K+ condition, staining buffer was replaced with 100mM K+ buffer containing 200nM DiBAC4(5). We captured 5 different fields at each time-point on fluorescent microscope, and analyzed the fluorescence intensity of all the cells by ImageJ.

实验结果（膜电位染色MK）：

Fig. Depolarization or Hyperpolarization of Membrane Potential May Affect the Activation of NLRP3 Inflammasome.THP-1 cells were stained with DiBAC4(5) to check the effect of CFTRinh-172 on MP. Representative images and relative fluorescence unit (RFU) plotting are shown. Each point was mean ± SEM of cells from at least 12 fields in 2 independent experiments. Scale bar, 20 μm.

注意事项

1）本品用有机溶剂（比如：DMSO）配制的储存液，请根据单次用量分装，置于≤-20℃避光保存。

2）荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭。

3）为了您的安全和健康，请穿实验服并戴一次性手套操作。

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— —Written/Edited by V. Shallan【版权归MKBio懋康所有】

上海懋康生物科技有限公司是一家涉足于生命科学和生物技术领域研究的试剂、仪器和实验室消耗品与实验服务工作，主要从事细胞生物学、植物学、分子生物学、免疫学、生物化学、蛋白组学。生物制药与诊断试剂研发生产等领域。 本公司秉承“以人为本，以诚为信、合同守信”的经营理念。坚持"品质保障"的原则为广大客户提供优质产品。