Thioflavin T ( Basic Yellow 1) 硫黄素T（碱性黄1）

产品关键词：

Thioflavin T硫黄素T；Basic Yellow 1 碱性黄1；Thioflavin S 硫黄素S；Amyloid plaques 淀粉样斑块；β sheet； CAS NO：2390-54-7；

产品信息

产品描述

硫黄素T（Thioflavin T），又称为碱性黄1（Basic Yellow 1），是一种具细胞膜渗透性的苯并噻唑染料，常用来鉴定样本内淀粉样纤维的存在与否。一旦与淀粉样纤维结合后荧光信号明显增强，结合后引起最大激发波长（385nm→450nm）和最大发射波长（445nm→482nm）的迁移。硫黄素T能用来监测堆积的β折叠，也能用来组织学和蛋白质鉴定。

产品特性

1）   CAS NO.：2390-54-7

2）   化学名：4-(3,6-Dimethyl-1,3-benzothiazol-3-ium-2-yl)-N,N-dimethylaniline chloride

3）   同义名：Basic Yellow 1; C.I. 49005; ThT; 碱性黄1；硫代磺素T；硫代黄色素 T；

4）   分子式：C17H19ClN2S

5）   分子量：318.86

6）   纯度：≥95%（HPLC）

7）   外观：黄色粉末

8）   溶解性：溶于DMSO、DMF、H2O（5mg/ml）

9）   化学结构式：

保存与运输方法

保存：2-8℃避光干燥保存，2年有效。

运输：室温运输。

应用示例

1、文献来源：Khurana R et al. Mechanism of thioflavin T binding to amyloid fibrils. J Struct Biol. 2005 Sep;151(3):229-38.

示例1：使用ThT的通用实验技术。a) Structure of ThT (top). The two planer segments of ThT whose mutual rotation defines chirality are also shown (bottom).(b) Early histology using Thioflavin-T to stain primary kidney amyloid.(c) TIRF microscopy image of branched glucagon fibrils stained with ThT.(d) Characteristic increase in ThT upon binding to amyloid fibrils.(e) Fibrillization kinetics of increased concentrations of a fibril-forming peptide. The rapid onset of fibrillization induced through seeding is also shown.

2、文献来源：Huang DS et al. Protective Effects of Wogonin against Alzheimer's Disease by Inhibition of Amyloidogenic Pathway. Evid Based Complement Alternat Med. 2017;2017:3545169.

Thioflavin T Assay（in solution）：The freshly prepared Aβ42 peptides (20 μM) with/without wogonin (10 μM) were added in a 100 μL sample containing 50 mM glycine and 40 μM Thioflavin T. The samples were loaded in a 96-well plate and florescent intensity of each sample was measured by excitation of 440 nm and emission of 490 mm every 30 min at 37°C for 16 hours.

3、文献来源：Marathe S et al. Jagged1 Is Altered in Alzheimer's Disease and Regulates Spatial Memory Processing. Front Cell Neurosci. 2017 Aug 9;11:220.

Jagged1 expression in brains and CSF of AD patients. Representative double fluorescent immunolabelings for Jagged1 (green) and Notch1 (red) counterstained with Thioflavin-T or DAPI (blue) on postmortem brain sections comprising the hippocampal CA fields from healthy age-matched controls and AD patients (A-C). In the healthy controls, Jagged1 is localized to the somata of neurons where also Notch1 is expressed. As expected, Thioflavin-T labeling is negligible (A,A′). AD sections show fibrillary aggregates (B,B′) and (C,C′) core plaques double positive for Notch1 and Thioflavin-T. [Misfolded β-sheets were stained with Thioflavin-T at a concentration of 100 mM diluted in water]

注意事项

1）   荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭。

2）   为了您的安全和健康，请穿实验服并戴一次性手套操作。

 — —Written/Edited by V. Shallan【版权归MKBio懋康所有】

上海懋康生物科技有限公司是一家涉足于生命科学和生物技术领域研究的试剂、仪器和实验室消耗品与实验服务工作，主要从事细胞生物学、植物学、分子生物学、免疫学、生物化学、蛋白组学。生物制药与诊断试剂研发生产等领域。 本公司秉承“以人为本，以诚为信、合同守信”的经营理念。坚持"品质保障"的原则为广大客户提供优质产品。