目录号 | MM1506-500ML | 售价 | 290.00元 | ||||||||||||||||||||||||||||||
规格 | 500ml | 运输温度 | 冰袋运输 | ||||||||||||||||||||||||||||||
其他名称 | 4%多聚甲醛(电镜专用) | 保存温度 | 4℃保存 | ||||||||||||||||||||||||||||||
CAS号 | 30525-89-4 | 有效期 | 4℃保存,3个月有效。开封后未用完请分装后置于-20℃保存,1年有效。 | ||||||||||||||||||||||||||||||
应用 | 电镜检测用固定液 | 订购数量 |
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产品简介: 4% Paraformaldehyde (EM Grade) 4%多聚甲醛(电镜专用)
产品信息
产品描述 多聚甲醛(Paraformaldehyde),化学名聚甲醛(polyoxymethylene),CAS NO. 30525-89-4,是一种甲醛聚合物粉末,本身不可固定组织。要用作有效的组织固定剂,需用热水溶解转化为甲醛溶液(单体甲醛)。
本品为不含甲醇的4%多聚甲醛溶液,主要由多聚甲醛、磷酸盐、去离子水组成,pH 7.4,适用于绝大多数组织和细胞的固定。作为免疫组织化学和培养细胞固定中最常用的固定液之一,本品能很好的保护组织和细胞的原有形态结构。本品特别开发用于电镜检测用样本的固定。
保存与运输方法 保存:4℃保存,3个月有效。开封后未用完请分装后置于-20℃保存,1年有效。 运输:冰袋运输。
使用方法 组织固定:室温或4℃浸泡固定4-12h,大标本应适当延长固定时间。依据组织的大小来决定固定时间,不要超过24h。 切片固定:室温或4℃固定10min-2h,视切片厚度而定。 细胞固定:培养细胞和细胞爬片室温或4℃固定10-15min,特殊情况除外。
注意事项 1) 新鲜组织取出后应及时固定。若无法及时固定,需保存在生理盐水中并尽快固定。 2) 温度对固定影响很明显,提高温度能加快固定但温度不宜太高。 3) 取材厚度不同固定时间也不同。避免过度延长固定时间,否则会引起生物大分子的过度交联。 4) 本品具有一定刺激性和腐蚀性,请在通风较好环境下小心操作,避免吸入。 5) 本品开启后请尽快用完,储存过久的溶液固定效果易下降。 6) 为了您的安全和健康,请穿实验服并戴一次性手套操作。
相关产品
参考文献 Cell invasion was detected using transwell chamber (Corning, NY, USA). Briefly, cells were seeded in the upper chamber pre-coated with matrigel at a density of 1 × 105/chamber. The medium containing 10% FBS was added in the lower chamber. After 24 h of culturing, cells on the upper chamber were removed. Cells in the lower chamber were fixed with 4% paraformaldehyde (Shanghai Maokang Biotechnology Co., Ltd, Shanghai, China) for 15 min, and stained with 0.1% crystal violet for 30 min. Positive stained cells were counted under a microscope (Olympus) at five randomly selected fields, and the invasion rate was calculated.
— —Written/Edited by V. Shallan【版权归MKBio懋康所有】
上海懋康生物科技有限公司是一家涉足于生命科学和生物技术领域研究的试剂、仪器和实验室消耗品与实验服务工作,主要从事细胞生物学、植物学、分子生物学、免疫学、生物化学、蛋白组学。生物制药与诊断试剂研发生产等领域。 本公司秉承“以人为本,以诚为信、合同守信”的经营理念。坚持"品质保障"的原则为广大客户提供优质产品。
引用文献: Cell invasion was detected using transwell chamber (Corning, NY, USA). Briefly, cells were seeded in the upper chamber pre-coated with matrigel at a density of 1 × 105/chamber. The medium containing 10% FBS was added in the lower chamber. After 24 h of culturing, cells on the upper chamber were removed. Cells in the lower chamber were fixed with 4% paraformaldehyde (Shanghai Maokang Biotechnology Co., Ltd, Shanghai, China) for 15 min, and stained with 0.1% crystal violet for 30 min. Positive stained cells were counted under a microscope (Olympus) at five randomly selected fields, and the invasion rate was calculated.
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