Nonyl Acridine Orange (NAO) 壬基吖啶橙

产品关键词：

Nonyl Acridine Orange (NAO)；壬基吖啶橙；JC-1 线粒体膜电位探针；MitoTracker® Green FM 绿色线粒体荧光探针；罗丹明123 Rhodamine 123；CAS NO：75168-11-5；

产品信息

【温馨提示】：见我司懋康生物（maokangbio）提供的线粒体荧光探针产品专题。

产品描述

壬基吖啶橙（Nonyl Acridine Orange，NAO），CAS NO. 75168-11-5，是一种吖啶橙（Acridine Orange，AO）衍生物，通常用作完整细胞线粒体内膜的荧光标志物。NAO一致认为能结合带负电荷的磷脂。NAO之所以能细胞内累积，与其同线粒体膜蛋白和/或脂质比如心磷脂的特定作用相关，基本上不依赖于线粒体膜电位。凋亡方面，线粒体膜电位的存在能用罗丹明123标记，而线粒体结构和完整性可用NAO来评估。NAO可通过流式检测来分析线粒体，也能用来描述多药耐药特征，以及测定凋亡过程中线粒体质量的变化。

应用示例

线粒体质量（Mitochondrial Mass）研究

METHOD：1 × 10⁵ FL5.12-AM cells were resuspended in 0.5 ml of complete medium containing 50 nM mitochondrial fluorescent dye acridine orange 10-nonyl bromide (NAO). After incubating of 30 min at 37 °C in dark, cells were immediately transferred to a tube on ice for analysis by flow cytometry. [Reference: Fan Y et al. Akt and c-Myc differentially activate cellular metabolic programs and prime cells to bioenergetic inhibition. J Biol Chem. 2010 Mar 5;285(10):7324-33.]

METHOD：Cells were incubated with or without H33342 or H33258 in 0.5 ml of 109cell/L HL-60 suspension in 12x75 mm plastic tubes at 37°C with 5% CO2. After incubation of cultured cells at various times with different amounts of H33342 or H33258, the delta psi or mitochondrial mass were measured with DiOC6(3) ornonyl acridine orange (NAO), respectively, added to achievea final concentration of 20 nM and incubated for 5 min at room temperature.Stock solutions of H33342, H33258, 4 μM DiOC6(3), or 4 μM NAO were dissolved in dimethyl-sulfoxide(DMSO). Finally, 2.5 μL of propidium iodide (PI) (1.0 mg/L stock) was added for the last 30s of incubation to assess cell viability. List mode files were collected for 25,000 events with a Coulter XL flow cytometer. [Reference: Chen JC et al. Mitochondrial membrane potential change induced by Hoechst 33342 in myelogenous leukemia cell line HL-60. Ann Clin Lab Sci. 2004 Autumn;34(4):458-66.]

产品特性

1）   CAS NO.：75168-11-5

2）   化学名：Acridinium, 3,6-bis(dimethylamino)-10-nonyl-, bromide

3）   同义名：Nonyl Acridine Orange, Acridine Orange 10-Nonyl Bromide, 10-Nonylacridine orange bromide；壬基吖啶橙，吖啶橙 10-溴壬烷，10-壬基吖啶橙溴化物

4）   分子式：C26H38BrN3

5）   分子量：472.50

6）   纯度：≥90%（HPLC）

7）   Ex/Em：496/524nm（in CH3OH）

8）   外观：橙色至红色粉末或固体

9）   溶解性：溶于DMSO，DMF，乙醇，微溶于水

10）化学结构式：

保存与运输方法

保存：室温避光干燥保存，2年有效。

运输：室温运输。

注意事项

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 — —Written/Edited by V. Shallan【版权归MKBio懋康所有】

上海懋康生物科技有限公司是一家涉足于生命科学和生物技术领域研究的试剂、仪器和实验室消耗品与实验服务工作，主要从事细胞生物学、植物学、分子生物学、免疫学、生物化学、蛋白组学。生物制药与诊断试剂研发生产等领域。 本公司秉承“以人为本，以诚为信、合同守信”的经营理念。坚持"品质保障"的原则为广大客户提供优质产品。