产品简介:
IBMX
(3-Isobutyl-1-methylxanthine) 3-异丁基-1-甲基黄嘌呤
产品标签
IBMX PDEs抑制剂；Forskolin
(Fsk) 佛司可林；Adenylate cyclase (AC)
腺苷酸环化酶；cAMP 环磷酸腺苷；Adenosine
receptor antagonist 腺苷受体拮抗剂；CAS NO：28822-58-4；
产品信息
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产品名称
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产品编号
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CAS NO.
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规格
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价格（元）
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IBMX (3-Isobutyl-1-methylxanthine)
3-异丁基-1-甲基黄嘌呤
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MZ2451-50MG
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28822-58-4
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50mg
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246
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IBMX
(3-Isobutyl-1-methylxanthine) 3-异丁基-1-甲基黄嘌呤
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MZ2451-100MG
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28822-58-4
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100mg
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386
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IBMX
(3-Isobutyl-1-methylxanthine) 3-异丁基-1-甲基黄嘌呤
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MZ2451-500MG
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28822-58-4
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500mg
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1286
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产品描述
IBMX
(3-Isobutyl-1-methylxanthine) ，中文名3-异丁基-1-甲基黄嘌呤，是一种普遍使用的和cAMP和cGMP磷酸二酯酶（PDEs）的非特异性抑制剂（PDE1，PDE2，PDE3，PDE4，PDE5，PDE7和PDE11的IC50分别为19，50，18，13，32，7和50μM）。PDE8A，PDE8B和PDE9对IBMX不敏感。通过抑制PDEs，IBMX增加细胞内cAMP和cGMP水平，激活环化核苷酸调节的蛋白激酶。IBMX能抑制神经内分泌上皮细胞α-肾上腺素受体介导的5-HT释放（IC50=1.3μM）。还是一种非选择性的腺苷受体拮抗剂。能加速小鼠成纤维细胞转化为脂肪细胞。体外还能促进神经祖细胞（NPC）分化。
产品特性
1） CAS NO：28822-58-4
2）化学名：3,7-Dihydro-1-methyl-3-(2-methylpropyl)-1H-purine-2,6-dione
3,7-二氢-1-甲基-3-(2-甲基丙基)- 1H-嘌呤-2,6-二酮；3-Isobutyl-1-methyl-2,6(1H,3H)-purinedione 3-异丁基-1-甲基-2,6(1H,3H)-嘌呤二酮；
3）同义名：3-Isobutyl-1-methylxanthine3-异丁基-1-甲基黄嘌呤；1-Methyl-3-isobutylxanthine1-甲基-3-异丁基黄嘌呤；IMX；MIX；Isobutylmethylxanthine；Methylisobutylxanthine；NSC165960；SC2964；
4）分子式：C10H14N4O2
5）分子量：222.24
6）纯度：≥99%
7）外观：白色或类白色粉末
8）溶解性：溶于乙醇（10mg/ml），DMSO（1M，微热助溶），甲醇（50mg/ml，微热助溶），DMF（5mg/ml），不溶于水。
9）化学结构图：
保存与运输方法
保存：室温干燥保存，或置于-20ºC长期干燥保存，至少2年有效。
运输：室温运输。
使用方法【源自文献，仅作参考】
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实验目的：3T3-L1小鼠前脂肪细胞诱导分化为成熟的脂肪细胞。
文献1，Chiadak JD
et al. Forskolin Inhibits Lipopolysaccharide-Induced Modulation of MCP-1 and
GPR120 in 3T3-L1 Adipocytes through an Inhibition of NFκB. Mediators Inflamm.
2016;2016:1431789. Epub 2016 Nov 2. PMID: 27881903
诱导方法：To induce
adipocyte differentiation,3T3-L1 murine preadipocyte cells (day 2) were
incubated for 60 h in DMEM supplemented with 10% fetal bovine serum and
containing 500 µM IBMX, 0.25 µM dexamethasone, and 10 µg/mL insulin. The
cells were then maintained in the culture medium supplemented with insulin
only and this media was changed every 2 days (day 5 and day 7) until complete
differentiation (monitored by lipid droplet accumulation under the microscope
and confirmed by Oil Red Coloration) had occurred (day 9).
文献2，Mudhasani R
et al. An Essential Role for Dicer in Adipocyte Differentiation.J Cell
Biochem. 2010 Jul 1;110(4):812-6. PMID: 20564208
诱导方法：Mouse
embryonic fibroblasts (MEFs)or preadipocytes were then plated at 50–60% confluence and
stimulated two days later (considered as day 0 [D0]) with a hormonal cocktail
composed of 0.25mM 3-Isobutyl-1-methylxanthine (IBMX), 0.1µM Dexamethasone,
Insulin at 10µg/ml and 1µM Rosiglitazone. Starting on day two (D2), the media
was replaced every two days with fresh DMEM supplemented with Insulin (10µg
/ml) and 1µM Rosiglitazone. Cells were harvested 7–9 days post-induction,
fixed in 10% formalin for Oil Red O (ORO) staining.
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实验目的：脐带血来源的间充质干细胞（Umbilical cord blood-derived mesenchymal stromal
cells, UCB-MSCs）诱导分化为脂肪细胞。
文献3，Rafieemehr H et al. Improving the neuronal
differentiation efficiency of umbilical cord blood-derived mesenchymal stem
cells cultivated under appropriate conditions. Iran J Basic Med Sci. 2015
Nov;18(11):1100-6. PMID: 26949497
诱导方法：To induce differentiation into adipocytes, cells were plated at
1,000 cells/cm2in 24-well plates in DMEM with 1 μM dexamethasone,
10 μg/ml insulin, 0.5 mM IBMX, and 100 μM indomethacin. After 2 wk of
adipogenic stimulation, cells were fixed in 5% PFA for 30 min and incubated
with Oil Red-O to stain lipid vacuoles.
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注意事项
1）本品不是临床药物，只能用于科研用途，不能用于诊断或临床用途。
2）为了您的安全和健康，请穿实验服并戴一次性手套操作。
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— —Written/Edited by V.
Shallan【版权归MKBio懋康所有】
上海懋康生物科技有限公司是一家涉足于生命科学和生物技术领域研究的试剂、仪器和实验室消耗品与实验服务工作，主要从事细胞生物学、植物学、分子生物学、免疫学、生物化学、蛋白组学。生物制药与诊断试剂研发生产等领域。 本公司秉承“以人为本，以诚为信、合同守信”的经营理念。坚持"品质保障"的原则为广大客户提供优质产品。
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