Alkaline Phosphatase Staining Kit II 碱性磷酸酶染色试剂盒

【温馨提示I】：见碱性磷酸酶产品专题，了解更多相关产品资料；

【温馨提示II】：见碱性磷酸酶活性检测试剂盒产品；

【温馨提示III】：见碱性磷酸酶常用生化底物及试剂盒产品；

基本介绍（Stemgent，00-0055）：

处于未分化状态的胚胎干细胞（ES）和诱导多潜能干细胞（iPS）典型特征为具高水平的碱性磷酸酶表达，同时结合其他的一些表面标志物，来显示未分化细胞具自我更新的潜能。碱性磷酸酶（ALP，或AP）是一种水解酶，碱性条件下能够将核苷酸、蛋白、甾醇等分子去磷酸化作用。

The undifferentiated state of embryonic stem (ES) and induced pluripotent stem (iPS) cells can be characterized by a high level of alkaline phosphatase (AP) expression which, along with the expression of other surface markers, indicates undifferentiated cells with the potential to self-renew.AP is a hydrolase enzyme responsible for dephosphorylating molecules such as nucleotides, proteins, and alkaloids under alkaline conditions. When fixed ES or iPS cells are stained using the AP Staining Kit, undifferentiated cells appear red or purple, whereas differentiated cells appear colorless.

结果呈现：

固定ES或iPS用碱性磷酸酶染色试剂盒（Stemgent，00-0055）染色后，未分化细胞呈红色或紫色，而分化细胞无色。

产品特性：

1. 试剂盒组分

1. 2. 保存和稳定性

4°C保存，不可冻融。按照指示保存收到货后至少能存放6个月。

1. 3. 质量控制

本碱性磷酸酶染色试剂盒以人和小鼠干细胞为对象进行功能测试，以确保产品质量。（The AP Staining Kit II is functionally tested on human and mouse ES cells to ensure product quality.）

订购信息：Stemgent原装进口，干细胞研究专家精品，品质认证，欢迎选购。

00-0055 Datasheet

00-0055 Staining Protocol

相关信息：自产碱性磷酸酶染色试剂盒，在保证优秀质量的情况下，提供更有竞争力的价格，欢迎选购。

参考文献：

Pease, S., Braghetta, P., Gearing, D., Grail, D., and Williams, R.L. (1990) Isolation of embryonic stem (ES) cells in media supplemented with recombinant leukemia inhibitory factor (LIF). Dev Biol. 141: 344-352.

Smith, A.G., Nichols, J., Robertson, M., and Rathjen, P.D. (1992) Differentiation inhibiting activity (DIA/LIF) and mouse development. Dev. Biol. 151: 339-351.

Takahashi, K., Tanabe K., Ohnuki, M., Narita, M., Ichisaka T., Tomoda K. and Yamanaka, S. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131: 861-872.

Yu, J., Vodyanik, M.A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J.L., Tian, S., Nie, J., Jonsdottir, G.A., Ruotti, V., Stewart, R., Slukvin, I.I., and Thomson, J.A. (2007) Induced pluripotent stem cell lines derived from human somatic cells. Science 318: 1917-1920.

Additional Publications

Momcilovic O; Sivapatham R; Oron TR; Meyer M; Mooney S; Rao MA; Zeng X. "Derivation, characteriazation, and neural differentiation of integration-free induced pluripotent stem cell lines from Parkinson's disease patients cfarrying SNCA, LRRK2, PARK2, and GBA mutations." PLoS ONE 11(5): e0154890. doi:10.1371/journal.pone.0154890 (2016)

 — — Written/Edited by V. Shallan【版权归MKBio懋康所有】

上海懋康生物科技有限公司是一家涉足于生命科学和生物技术领域研究的试剂、仪器和实验室消耗品与实验服务工作，主要从事细胞生物学、植物学、分子生物学、免疫学、生物化学、蛋白组学。生物制药与诊断试剂研发生产等领域。 本公司秉承“以人为本，以诚为信、合同守信”的经营理念。坚持"品质保障"的原则为广大客户提供优质产品。