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当前位置: 首页> 产品中心> 蛋白质研究 > 酶活性检测 > Stemgent Alkaline Phosphatase Staining Kit II
Stemgent Alkaline Phosphatase Staining Kit II
目录号 00-0055 售价 2350.00元
规格 50 assays 运输温度 冰袋运输
其他名称 保存温度 4°C保存,不可冻融
CAS号 N/A 有效期 6个月
应用 碱性磷酸酶染色(定性研究) 订购数量
产品简介:

Alkaline Phosphatase Staining Kit II 碱性磷酸酶染色试剂盒

 

【温馨提示I】:碱性磷酸酶产品专题,了解更多相关产品资料;

【温馨提示II】:碱性磷酸酶活性检测试剂盒产品

【温馨提示III】:见碱性磷酸酶常用生化底物及试剂盒产品;

 

基本介绍(Stemgent,00-0055):

处于未分化状态的胚胎干细胞(ES)和诱导多潜能干细胞(iPS)典型特征为具高水平的碱性磷酸酶表达,同时结合其他的一些表面标志物,来显示未分化细胞具自我更新的潜能。碱性磷酸酶(ALP,或AP)是一种水解酶,碱性条件下能够将核苷酸、蛋白、甾醇等分子去磷酸化作用。

The undifferentiated state of embryonic stem (ES) and induced pluripotent stem (iPS) cells can be characterized by a high level of alkaline phosphatase (AP) expression which, along with the expression of other surface markers, indicates undifferentiated cells with the potential to self-renew.AP is a hydrolase enzyme responsible for dephosphorylating molecules such as nucleotides, proteins, and alkaloids under alkaline conditions. When fixed ES or iPS cells are stained using the AP Staining Kit, undifferentiated cells appear red or purple, whereas differentiated cells appear colorless.

 

结果呈现:

固定ES或iPS用碱性磷酸酶染色试剂盒(Stemgent,00-0055)染色后,未分化细胞呈红色或紫色,而分化细胞无色。

 

产品特性:

1. 试剂盒组分

组分                                   

规格            

编号             

Fix Solution

25 ml

09-0042

AP Staining Solution A

10 ml

09-0043

AP Staining Solution B

10 ml

09-0065

AP Staining Solution C

10 ml

09-0066

  1. 2. 保存和稳定性

4°C保存,不可冻融。按照指示保存收到货后至少能存放6个月。

  1. 3. 质量控制

本碱性磷酸酶染色试剂盒以人和小鼠干细胞为对象进行功能测试,以确保产品质量。(The AP Staining Kit II is functionally tested on human and mouse ES cells to ensure product quality.)

 

 

订购信息:Stemgent原装进口,干细胞研究专家精品,品质认证,欢迎选购。

品牌          

名称                                                   

货号           

规格          

目录价(元)

货期    

Stemgent

Alkaline Phosphatase Staining Kit II

00-0055

50 assays

2350

咨询

00-0055 Datasheet

00-0055 Staining Protocol

 

相关信息:自产碱性磷酸酶染色试剂盒,在保证优秀质量的情况下,提供更有竞争力的价格,欢迎选购。

品牌          

名称                                                

货号               

规格       

目录价(元)

货期     

MKbio

Alkaline Phosphatase Staining Kit

碱性磷酸酶染色试剂盒

MP7503-50T

50T

2182

现货

MKbio

MP7503-100T

100T

4252

现货

 

参考文献:

Pease, S., Braghetta, P., Gearing, D., Grail, D., and Williams, R.L. (1990) Isolation of embryonic stem (ES) cells in media supplemented with recombinant leukemia inhibitory factor (LIF). Dev Biol. 141: 344-352.

Smith, A.G., Nichols, J., Robertson, M., and Rathjen, P.D. (1992) Differentiation inhibiting activity (DIA/LIF) and mouse development. Dev. Biol. 151: 339-351.

Takahashi, K., Tanabe K., Ohnuki, M., Narita, M., Ichisaka T., Tomoda K. and Yamanaka, S. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131: 861-872.

Yu, J., Vodyanik, M.A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J.L., Tian, S., Nie, J., Jonsdottir, G.A., Ruotti, V., Stewart, R., Slukvin, I.I., and Thomson, J.A. (2007) Induced pluripotent stem cell lines derived from human somatic cells. Science 318: 1917-1920.


Additional Publications

Momcilovic O; Sivapatham R; Oron TR; Meyer M; Mooney S; Rao MA; Zeng X. "Derivation, characteriazation, and neural differentiation of integration-free induced pluripotent stem cell lines from Parkinson's disease patients cfarrying SNCA, LRRK2, PARK2, and GBA mutations." PLoS ONE 11(5): e0154890. doi:10.1371/journal.pone.0154890 (2016)

 



 — — Written/Edited by V. Shallan【版权归MKBio懋康所有】

 

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