2-NBDG  葡萄糖摄取荧光探针  

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产品关键词：

2-NBDG；6-NBDG；Glucose uptake and transport probe 葡萄糖摄取和转运探针；Cell viability 细胞活力指示剂；Topical contrast reagent 局部对比试剂；3T3-L1 adipocytes 小鼠脂肪细胞；C2C12 muscle cells 小鼠成肌细胞；CAS:186689-07-6；

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【温馨提示】：见我司整理的葡萄糖类似物/葡萄糖摄取探针2-NBDG产品专题。

基本描述：

2-NBDG，英文全名2-Deoxy-2- [(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-D-glucose，CAS NO：186689-07-6，一种脱氧葡萄糖类似物的荧光素，常常用来直接监测活细胞和组织对葡萄糖的摄取，判断细胞活力的指标之一。还可用作监测瘤形成的一种局部对比试剂。可用实时共聚焦、高分辨率或宽视野荧光显微镜，以及流式细胞仪来检测。尽管2-NBDG荧光对环境比较敏感，但约在465/540nm下表现出最大激发和最大发射波长，使用荧光素（如FITC）的光学滤片来进行结果观察即可。

产品特性：

1）CAS NO：186689-07-6

2）同义名：NBD-Glucose; 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose; 2-Deoxy-2- [(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-D-glucose

3）分子式：C12H14N4O8

4）分子量：342.26

5）纯度：≥98% (mixture of α and β)

6）外观：灰白色或橘色固体

7）溶解性：溶于DMSO（10mg/ml）、无水乙醇（20mg/ml）、溶于PBS，pH 7.2（10mg/ml）

8）化学结构图： 

常见问题（FAQs）【详见maokangbio官网】：

1、2-NBDG或6-NBDG的储存液制备方法？

往1mg粉末内加入100μl无水DMSO或乙醇，充分溶解混匀，制备成10mg/ml储存液，按照单次用量分装后，置于-20ºC避光冻存。若配制成水溶液溶液，建议存放不要超过一天。

若需要延长保存周期，可以粉末形式置于-20ºC保存。先用乙醇溶解粉末，然后分装成小量装入独立瓶内，使用真空泵将溶剂抽干即可。此时不推荐用DMSO或DMF为溶剂，因挥发性比较差。

2、2-NBDG或6-NBDG的工作浓度？

2-NBDG的常用工作浓度范围10-600μM。细菌和酵母菌染色建议工作浓度范围为100-600μM，原代和传代培养哺乳动物细胞建议工作浓度范围为10-200μM。

6-NBDG的常用工作浓度范围30-300μM。

细胞在合适的温度和时间内孵育，以充分检测绿-黄色荧光。摄取能力强弱受温度影响，孵育时间有几秒钟，到30min，甚至更长，取决于探针终浓度、细胞类型、培养条件和一些其他因素。

相关产品1：更大包装或产品技术问题，请来电/在线咨询，电话：021-54736159。QQ：2971634497。

相关产品2：更大包装或产品技术问题，请来电/在线咨询，电话：021-54736159。QQ：2971634497。

注意事项

为了您的安全和健康，请穿实验服并戴一次性手套操作。

本产品仅能用作科研用途，不可他用。

引用文献

[1]Zhang Yh et al. A New Possible Mechanism by Which Punicalagin Protects against Liver Injury Induced by Type 2 Diabetes Mellitus: Upregulation of Autophagy via the Akt/FoxO3a Signaling Pathway. J. Agric. Food Chem. 2019, 67, 50, 13948–13959

2-NBDG was purchased from Shanghai maokang biotechnology co.,ltd

[2]Zhang, Lx., Song, Jw., Ma, Yd. et al. Expression of SGLT1 in the Mouse Endometrial Epithelium and its Role in Early Embryonic Development and Implantation. Reprod. Sci. (2021). https://doi.org/10.1007/s43032-021-00480-y

2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl) amino]-D-glucose (2-NBDG; MKbio, 186689-07-6)

[3]Qian H, Kuang Y, Su J, Chen M, Chen X, Lv C, Chen W and Zhu W (2021) Reductive Effect of Acitretin on Blood Glucose Levels in Chinese Patients With Psoriasis. Front. Med. 8:764216. doi: 10.3389/fmed.2021.764216

2-NBDG (MKBio, Shanghai, China, CAS NO: 186689-07-6), a green, fluorescent deoxyglucose analog, was used to monitor the cell glucose uptake using flow cytometry or fluorescence microscopy, as an indicator of cellular viability. To detect the uptake of 2-NBDG, 1 × 10⁵ cells/well were seeded in a 12-well plate, six of which were incubated with complete DMEM as the control, and the remaining six wells incubated with DMEM and acitretin 5000 ng/mL as the acitretin group. After 36 h, the cells were washed three times with 200 μL cold phosphate-buffered saline (PBS), treated with 0.5 mM 2-NBDG for 30 min, then washed thrice with cold PBS. This process was repeated three times.

The uptake of 2-NBDG by both HaCaT and HepG2 cells was observed by fluorescence microscopy (Nikon Eclipse TS2R, Japan). The cells were then resuspended with 200 μL PBS per well and assessed using the BD Aucrri C6 flow cytometer, as previously described.

[4] Mengting Wang, Haiguang Mao, Jianchu Chen, Qiang Li, Wei Ma, Nan Zhu, Lili Qi, Jinbo Wang, Chinese bayberry (Myrica rubra Sieb. et Zucc.) leaves proanthocyanidins alleviate insulin-resistance via activating PI3K/AKT pathway in HepG2 cells, Journal of Functional Foods,Volume 99,2022,105297,ISSN 1756-4646,https://doi.org/10.1016/j.jff.2022.105297.

2-NBDG was brought from Maokang Biotechnology (Shanghai, China). The glucose uptake assay was conducted according to the previous report with some modifications (Nie et al., 2017). After treatment, the cells were incubated with 100 μM 2-NBDG at 37 °C for 1 h and washed three times by PBS. The images were obtained using a fluorescence microscope (Olympus, Tokyo, Japan) at the condition of Ex/Em = 470/545 nm.

[5] Gong PX, Li QY, Wu YC, Lu WY, Zeng J, Li HJ. Structural elucidation and antidiabetic activity of fucosylated chondroitin sulfate from sea cucumber Stichopus japonicas. Carbohydr Polym. 2021 Jun 15;262:117969. doi: 10.1016/j.carbpol.2021.117969. Epub 2021 Mar 20. PMID: 33838834.

 Glucose uptake was measured using 2-NBDG (Maokang, Shanghai, China) as described previously ( Xia et al., 2020 ). Briefly, cells (1 × 10 5 cells/mL) were seeded in 6-well plates. After treatments, 2-NBDG was added to the medium at a final concentration of 25 μM, and incubated at 37 °C for 1 h.

 — —Written/Edited by V. Shallan【版权归MKBio懋康所有】

      上海懋康生物科技有限公司是一家涉足于生命科学和生物技术领域研究的试剂、仪器和实验室消耗品与实验服务工作，主要从事细胞生物学、植物学、分子生物学、免疫学、生物化学、蛋白组学。生物制药与诊断试剂研发生产等领域。 本公司秉承“以人为本，以诚为信、合同守信”的经营理念。坚持"品质保障"的原则为广大客户提供优质产品。