FITC-PNA (FITC labeled Peanut Agglutinin)

FITC标记花生凝集素

产品信息

【温馨提示】：凝集素类产品做切片的染色，如需要做封闭的话，请选择Carbo-Free Blocking Solution (10×) 凝集素专用封闭/稀释液（货号：MP6342），避免使用含BSA、血清或酪蛋白的封闭液，否则会引起高背景和非特异性染色。

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5） 更多凝集素产品逐渐开发中。。。。。。

产品描述

花生凝集素（Peanut Agglutinin，PNA）约占常见花生中重量的0.15%，是一种糖类识别蛋白，由4个相同亚基（不含糖类）构成的蛋白，分子量为110kDa。优先结合T抗原（Galβ1, 3GalNAc），这一结构存在于大量糖复合物上，比如M血型和N血型，神经节苷酯，以及许多其他可溶的和膜结合的糖蛋白和糖脂。除掉某些例外，PNA的受体序列通常是唾液酸化的，防止凝集素结合到寡糖上。唾液酸即使不会直接结合受体糖，但可能抑制结合。稀释液内的钙离子能提高PNA结合到受体上，可能通过中和受体序列邻近的唾液酸残基上的负电荷来发挥作用。

花生凝集素（Peanut Agglutinin，PNA）适用于鉴别正常和肿瘤组织，以及评估移行粘膜上肿瘤的恶性程度。另外，PNA结合能用于测定淋巴组织上的细胞成熟度，用来区分男人和实验室动物上各种淋巴细胞亚群，以及用来测定大量疾病下的淋巴细胞群水平。PNA还应用于小鼠干细胞的分选，用于穿透组织相容性屏障的骨髓移植。

本品是FITC标记的花生凝集素（FITC-Peanut Agglutinin, FITC-PNA），Ex/Em=495/515nm，亲和纯化所得，基本不含未标记的荧光素。建议工作浓度范围是5-20μg/ml。

产品特性

1） 同义名：Peanut Agglutinin,FITC Conjugate; FITC labeledPNA Lectin; FITC labeled Peanut Lectin; Peanut Agglutinin, Fluorescein; 花生凝集素，FITC标记；

2） 糖类特异性：半乳糖（Galactose）

3） 外观：溶液（溶于10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.1 mM CaCl2, 0.01mM MnCl2, 0.08% sodium azide）

4） 蛋白浓度：5mg/ml

5） 最大激发范围：495-500nm

6） 最大发射范围：514-521nm

7） 抑制和/或洗脱糖类：200mM半乳糖

8） 应用：IF、糖生物学

保存与运输方法

保存：2-8℃避光保存，至少1年有效。 

运输：冰袋运输。

注意事项

1） 本品置于2-8℃长期保存可能会产生沉淀，建议使用前请37℃温育数分钟，之后离心吸取上清使用，能够有效降低非特异性的背景染色。此操作基本不会对产生性能造成负影响。

2） 懋康生物提供两种荧光标记的PNA，一种是FITC-PNA（#MP6327），一种是罗丹明-PNA（#MP6328）。

3） 为了您的安全和健康，请穿实验服并戴一次性手套操作。

相关产品

 — —Written/Edited by V. Shallan【版权归MKBio懋康所有】

上海懋康生物科技有限公司是一家涉足于生命科学和生物技术领域研究的试剂、仪器和实验室消耗品与实验服务工作，主要从事细胞生物学、植物学、分子生物学、免疫学、生物化学、蛋白组学。生物制药与诊断试剂研发生产等领域。 本公司秉承“以人为本，以诚为信、合同守信”的经营理念。坚持"品质保障"的原则为广大客户提供优质产品。

应用文献：

[1]He, J., Li, J., Lin, Q. et al. Anti-CD20 treatment attenuates Th2 cell responses: implications for the role of lung follicular mature B cells in the asthmatic mice. Inflamm. Res. 73, 433–446 (2024). https://doi.org/10.1007/s00011-023-01847-4

FITC-PNA (Maokang, Shanghai, China),

[2] Wang, J.-R.; Huang, F.; Niu, P.; Cheng, H.; Qu, H.-M.; Li, X.-P.; Wang, X.-Y.; Wang, J.; Suo, J.-J.; Fang, D.; et al. Comparison of Superovulated Embryo Quality in Simmental Cattle Inseminated with 0 °C-Refrigerated and Liquid Nitrogen-Frozen Semen. Biology 2025, 14, 658. https://doi.org/10.3390/biology14060658

2.6. Sperm Acrosome and Plasma Membrane Integrity Detection

The FITC-PNA/DAPI staining method (MP6327; MX4208; Maokang Biotechnology Co., Ltd., Shanghai, China) was employed in this study. Initially, 20 μL of sperm from various groups was placed on a glass slide, air-dried, and fixed with a 2% paraformaldehyde solution (MM1515; Maokang Biotechnology Co., Ltd., Shanghai, China) for 10 min, followed by air-drying. Subsequently, 2 mL of PBS was added to the slide and incubated for 10 min, being repeated twice to eliminate residual paraformaldehyde. Next, 30 μL of the FITC-PNA working solution was evenly applied to cover the sperm sample, incubated in darkness at 37 °C for 30 min, and washed three times with PBS to remove excess dye. Following this, 30 μL of DAPI staining solution was uniformly applied to the slide, incubated in darkness at room temperature for 5–10 min, and immediately observed and imaged under a fluorescence microscope (Nikon Ti2-U), ensuring that light exposure was strictly avoided throughout the process. Sperm exhibiting complete bright green fluorescence in the anterior acrosome were classified as intact acrosome sperm, whereas those with incomplete or absent fluorescence were considered acrosome-damaged. Imaging was conducted in complete darkness. The acrosome integrity rate was calculated as follows: Acrosome Integrity Rate (%) = (Number of intact acrosome sperm/Total sperm count) × 100%. For plasma membrane integrity assessment, the SYBR 14/PI staining method (MX4239; MX4205; Maokang Biotechnology Co., Ltd., Shanghai, China) was utilized following the same protocol as the FITC-PNA/DAPI staining. Sperm exhibiting green fluorescence were identified as viable, while red fluorescence indicated a loss of membrane integrity. The membrane integrity rate was calculated as follows: Membrane Integrity Rate (%) = (Number of membrane-intact sperm/Total sperm count) × 100%. Sperm were observed across at least five randomly selected fields, and each sample was analyzed in triplicate.

[3] Lisha Yin, et al. m⁶A Reader hnRNPA2B1 Modulates Late Pachytene Progression in Male Meiosis Through Post-Transcriptional Control. Advancement of science Volume12, Issue38 October 13, 2025

PNA (Maokangbio, MP6328) was used to identify tubule stage. Antibodies used in this study are provided in Table S5 (Supporting Information).