化学名：6,6'-[(3,3-Dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[4-amino-5-hydroxy-1,3- napthalenedisulphonic acid] tetrasodium salt

同义名：CSB6B; Direct Blue 1; Pontamine sky blue; C.I. 24410; 直接湖蓝6B；滂胺天蓝；芝加哥天蓝6B；

CAS NO：2610-05-1

MDL NO：MFCD00004020

分子式：C₃₄H₂₄N₆Na₄O₁₆S₄

分子量：992.79 g/mol

纯度：≥90%（MS4073B）；≥95%（MS4073C）

外观：蓝色/黑色固体

溶解性：易溶于水（≥10mg/ml），微溶于溶纤素，不溶于其他有机溶剂

化学结构式：

          质量

溶剂体积

浓度

1mg

5mg

10mg

1mM

1.0073 mL

5.0363 mL

10.0725 mL

5mM

0.2015 mL

1.0073 mL

2.0145 mL

10mM

0.1007 mL

0.5036 mL

1.0073 mL

文献1，Yifa O, Weisinger K, Bassat E, Li H, Kain D, Barr H, Kozer N, Genzelinakh A, Rajchman D, Eigler T, Umansky KB, Lendengolts D, Brener O, Bursac N, Tzahor E. The small molecule Chicago Sky Blue promotes heart repair following myocardial infarction in mice. JCI Insight. 2019 Nov 14;4(22):e128025. doi: 10.1172/jci.insight.128025. PMID: 31723055; PMCID: PMC6948869.

体外研究（细胞水平）：

细胞类型（Cell Type）：Cardiac cells and Bone marrow cells

配制方法（Preparation）：Chicago Sky Blue 6B (CSB) was dissolved in DDW to 10 μM for all in vitro cardiac cell culture experiments, and 50 μM for BMDM experiments.

实验方法（Assay）：Bone marrow cells were isolated and plated in macrophage differentiation medium. After 6 days of culture, the medium was aspirated and replaced with fresh macrophage differentiation medium with the addition of 10 ng/mL LPS. After 24 hours the LPS-containing medium was aspirated, cells were washed twice with PBS, and were treated with 50 μM CSB in macrophage differentiation medium. Twenty-four hours later the cells were washed and RNA was purified.

体内研究：

动物模型（Animal Model）：12-week-old Hsd:ICR female mice (Envigo RMS)

给药剂量（Dosages）：In vivo: CSB was diluted in PBS. Injection by i.m.: 50 μL of 100 μM stock solution, which is 0.005 μmoles (5 μg). Injections by i.v.: 100 μL of 100 μM stock solution, which is 0.01 μmoles (10 μg). 

实验方法（Assay）：Mice were sacrificed 21 days after MI following 7 injections of CSB. The first injection was 50 μL of 100 μM CSB and was injected i.m. into the myocardium. Six additional injections were 100 μL of 100 μM CSB and were injected i.v. into the tail vein. The last i.v. injection was given 14 days after MI. PBS-treated mice were examined for comparison. A total of 6 CSB-treated and 5 PBS-treated mice were examined.

文献2，Goolam, M., Xypolita, ME., Costello, I. et al. The transcriptional repressor Blimp1/PRDM1 regulates the maternal decidual response in mice. Nat Commun 11, 2782 (2020). https://doi.org/10.1038/s41467-020-16603-z

体内研究：

动物模型（Animal Model）：Female C57BL/6 mice (6–10 weeks of age)

药物配制（Preparation）：1% (w/v) Chicago Sky Blue 6B in PBS

实验方法（Assay）：To visualise implantation sites, mice were injected intravenously with 0.1 ml of 1% (w/v) Chicago Sky Blue 6B in PBS under anaesthetic and sacrificed by CO₂ asphyxiation 10 min later. Individual decidua were removed from the uterus and shaken in 25 μl of formamide for 2 days at room temperature (RT), protected from light to extract the dye. After clearing by centrifugation (16,000 × g for 5 min), dye content in formamide extracts was quantitated spectrophotometrically using an ND1000 Nanodrop at 618 nm relative to a standard curve of Chicago Sky Blue dye dissolved in formamide.

文献3，Afshar P, Larijani LV, Rouhanizadeh H. A comparison of conventional rapid methods in diagnosis of superficial and cutaneous mycoses based on KOH, Chicago sky blue 6B and calcofluor white stains. Iran J Microbiol. 2018 Dec;10(6):433-440. PMID: 30873272; PMCID: PMC6414738.

体外研究（细胞水平）：

细胞类型（Cell Type）：Cardiac cells and Bone marrow cells

配制方法（Preparation）：Chicago Sky Blue 6B (CSB) was dissolved in DDW to 10 μM for all in vitro cardiac cell culture experiments, and 50 μM for BMDM experiments.

实验方法（Assay）：Bone marrow cells were isolated and plated in macrophage differentiation medium. After 6 days of culture, the medium was aspirated and replaced with fresh macrophage differentiation medium with the addition of 10 ng/mL LPS. After 24 hours the LPS-containing medium was aspirated, cells were washed twice with PBS, and were treated with 50 μM CSB in macrophage differentiation medium. Twenty-four hours later the cells were washed and RNA was purified.

货号

名称

规格

MS4073A-5G

Chicago Sky Blue 6B, BS Grade 芝加哥天蓝6B（滂胺天蓝）

5g

MS4073B-25G

Chicago Sky Blue 6B, 90% 芝加哥天蓝6B（滂胺天蓝）

25g

MS4073C-1G

Chicago Sky Blue 6B, 95% 芝加哥天蓝6B（滂胺天蓝）

1g

MS4007-5G

Evans Blue 伊文思蓝（埃文斯蓝）

5g

MS4049-100ML

Evans Blue Stain (0.5%) 伊文思蓝染色液（0.5%）

100ml

MS4064-100ML

Evans Blue Stain (2%) 伊文思蓝染色液（2%）

100ml

MS4001-5G

TTC 2,3,5-氯化三苯基四氮唑

5g

MM1006-100ML

TTC Stain Solution (2%) TTC染色液（2%）

100ml