| 目录号 | MX4826-250MG | 售价 | 1860.00元 | |||||||||||||||||||||||||||||||||||||||
| 规格 | 250mg | 运输温度 | 冰袋运输。 | |||||||||||||||||||||||||||||||||||||||
| 其他名称 | Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) dichloride 三(4,7-二苯基-1,10-菲咯啉)二氯化钌(II)；Ru(ddp)； | 保存温度 | 2-8ºC避光干燥保存，亦可置于-20℃避光干燥长期保存，至少1年有效。 | |||||||||||||||||||||||||||||||||||||||
| CAS号 | 36309-88-3 | 有效期 | 1年 | |||||||||||||||||||||||||||||||||||||||
| 应用 | 氧指示探针 | |||||||||||||||||||||||||||||||||||||||||
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产品简介: [Ru(dpp)3]Cl2 (Luminescent oxygen sensor)氧指示探针（金标产品，享发文奖励）
重要提醒（购买或初次使用前）请务必查阅：
一、荧光染料（粉末形式，特别是对氧敏感探针）配制储存液的注意事项
1）荧光探针在固体（粉末）状态很稳定，按照说明书要求温度来保存，效期内使用即可。
2）荧光探针用有机溶剂比如：DMSO，溶解配制成储存液之后，一般来说，放到-20℃保存（2-3个月内使用，甚至更短，具体可咨询）；放到-80℃保存（6个月内使用，具体可咨询）。前提是，使用的有机溶剂必须是高质量且无水的，特别是DMSO，必须是新鲜开封。
3）配制的储存液请务必用密封性好且螺旋盖的低容量冻存管保存（不可用EP管），至少按照5-10ul/管来分装，避光冻存。
4）对于某些特殊化合物（对空气敏感或存在不稳定结构），可能保存周期特别短，甚至只能当天使用。这些化合物说明书上会有说明。
有更多信息，请联系我司工作人员来核实。
二、荧光染料（以溶于有机溶剂的储存液形式提供）的注意事项
1） 以溶于有机溶剂的储存液形式的荧光探针相对来说是比较稳定的化合物；但收到这类产品，也需用户根据单次用量（5-10ul/管来分装），-20℃以下密封避光保存，减少反复冻融次数。
2） 请务必用密封性好且螺旋盖的低容量冻存管保存（不可用EP管）。务必避光。
有更多信息，请联系我司工作人员来核实。
产品标签 [Ru(dpp)3]Cl2；Ru(ddp)；Oxygen sensor；氧指示探针；SOSG 单线态氧荧光探针；CAS: 36309-88-3；
产品信息
产品描述 [Ru(dpp)3]Cl2（中文全名：三(4,7-二苯基-1,10-菲咯啉)二氯化钌(II)，英文全名：(Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) dichloride），是一款发光探针（最大吸收波长absorption λmax：455 nm，发光最大吸收波长luminescence λmax: 613 nm），普遍用于氧（Oxygen）的检测和定量[1]。由于动态淬灭的作用，分子氧引起[Ru(dpp)3]Cl2的光显著降低。因此，通过测定光强度或衰减时间来检测分子氧[2]。[Ru(dpp)3]Cl2还被用于优化光学氧传感器，用于测定组织和皮肤瘤的氧流量，以及用于氧成像分析[3-6]。
产品特性 1）CAS NO.：36309-88-3 2）化学名：(OC-6-11)-tris(4,7-diphenyl-1,10-phenanthroline-κN1,κN10)-ruthenium(2+), dichloride 3）同义名：Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) dichloride三(4,7-二苯基-1,10-菲咯啉)二氯化钌(II)；Ru(ddp)； 4）分子式：C72H48Cl2N6Ru 5）分子量：1169.17 6）外观：固体 7）溶解性：溶于DMSO（25mg/ml）、乙醇（25mg/ml）、DMF（25mg/ml） 8）化学结构式：
保存与运输方法 保存：2-8ºC避光干燥保存，亦可置于-20℃避光干燥长期保存，至少1年有效。 运输：冰袋运输。
注意事项 1) 本品对空气敏感，粉末内置惰性气体保护。建议配制的储存液也保存于惰性气体下。 2)为了您的安全和健康，请穿实验服并戴一次性手套操作
应用示例（仅作参考） 1.文献来源：Cheng, Q., Li, ZH., Sun, YX. et al. Controlled synthesis of a core-shell nanohybrid for effective multimodal image-guided combined photothermal/photodynamic therapy of tumors. NPG Asia Mater 11, 63 (2019). Editedby MKBIO
①溶液内的氧生成：为了评估PCN外壳对PB核催化效率的影响，取3组具不同PCN外壳厚度的PB@PCN，加入24孔板[孔内含3% H2O2和[Ru(dpp)3]Cl2（10mg/L，10μL）]，37℃孵育15min。另外，PBS, PB (20mg/L)和PB@PCN 3mL + 808 nm辐射处理作为对照组。用小动物荧光成像系统（IVIS, λex= 488 nm, λem = 620 nm）来记录荧光。
②细胞内的氧生成：CT26细胞接种在24孔板，37℃培养24h，之后每孔加入Ru(dpp)3]Cl2（10mg/L，10μL）。12h之后，PBS清洗，然后加入200μL 1640培养基。之后，PBS, PB (20 mg/L)或PB@PCN（20mg/L）加入孔内，继续孵育24h。PBS清洗，用小动物荧光成像系统（λex= 488 nm, λem = 620 nm）记录荧光。
Fig . d Detection of PB@PCN-catalyzed O2 generation with different concentrations of H2O2 (0 (1), 1.5 w% (2), 3 w% (3), and 6 w% (4)) by ([Ru(dpp)3]Cl2).
2.文献来源：Liu XL, Dong X, Yang SC, et al. Biomimetic Liposomal Nanoplatinum for Targeted Cancer Chemophototherapy. Adv Sci (Weinh). 2021;8(8):2003679. Editedby MKBIO Published 2021 Mar 1.
①细胞内的氧生成：4T1细胞（5 × 103/孔）接种在96孔板（(1% O2, 5% CO2 balanced with N2），缺氧培养24h。细胞内加入[Ru(dpp)3]Cl2（5 × 10−6 M）处理4h，然后加入nano-Pt/VP@MLipo (200 × 10−9 M VP, 2.86 µg/mL Pt)处理4h。之后用Ex 488 nm, Em 610 nm观察细胞荧光，共聚焦荧光显微镜（CLSM）拍照。
Fig C) Nano‐Pt increased the intracellular oxygenation as examined using the oxygen indicator [Ru(dpp)3]Cl2. The red fluorescence of the dye was quenched in the presence of oxygen.
相关产品
— —Written/Edited by V. Shallan【版权归MKBio懋康所有】
上海懋康生物科技有限公司是一家涉足于生命科学和生物技术领域研究的试剂、仪器和实验室消耗品与实验服务工作，主要从事细胞生物学、植物学、分子生物学、免疫学、生物化学、蛋白组学。生物制药与诊断试剂研发生产等领域。 本公司秉承“以人为本，以诚为信、合同守信”的经营理念。坚持"品质保障"的原则为广大客户提供优质产品。
引用文献:
[1] Shu Zhang et al. All-in-one device for mapping the interactive effects of photodynamic therapy dosimetry in tumor gaseous microenvironment. Journal of Photochemistry and Photobiology B: Biology. Volume 239, February 2023, 112645
The O 2 gradient generated in the 96-well plate by the use of the gas-gradient-generating device was determined by an O 2 -sensitive fluorescent dye, tris (4,7-diphenyl-1,10-phenanthroline)ruthenium (II) dichloride ( [ (Ru (dpp) 3 )]Cl 2 ), Shanghai Maokang Biotechnology Co., Ltd.), the fluorescence of which was reduced by molecular O 2.
[2]Xu Y, Yang L, Wang C, Sun W, Zheng Y, Ou B, Wu L, Shi L, Lin X, Chen W. Ferroptosis boosted oral cancer photodynamic therapy by carrier-free Sorafenib-Ce6 self-assembly nanoparticles. J Control Release. 2024 Feb;366:798-811. doi: 10.1016/j.jconrel.2023.12.056. Epub 2024 Jan 25. PMID: 38184236. [Ru(dpp)3]Cl2 (RDPP) luminescent oxygen sensor (Catalog number: MX4826-100MG) was bought from Maokang Biotechnology Co., Ltd. (Shanghai, China).
[3]Zhang, S., Zhang, H., Song, P. et al. A biodegradable nanodrug with highly efficient treatment effect by remodeling tumor microenvironment and manipulating energy metabolism against colorectal cancer. Sci. China Mater. 67, 331–342 (2024). https://doi.org/10.1007/s40843-023-2693-7 The indicator [Ru(dpp)3]Cl2 was obtained from Maokang Biotechnology Co., Ltd. (China).
[4] He, Zhijing et al. A bimetallic dual-targeting nanoplatform for combinational ferroptosis activation/epigenetic regulation/photothermal therapy against breast cancer and tumor microenvironment remodeling. Chemical Engineering Journal, Volume 479, id.147466 DOI:
Tris (4,7-diphenyl-1,10-phenanthroline) ruthenium (Ⅱ) dichloride complex (Ru(dpp) 3 Cl 2), C11 BODIPY 581/591 and ROSGreen TM H 2 O 2 probe were purchased from Shanghai Maokang Biotechnology Co., Ltd.
[5] Lijian Cao et al. The direct catalytic synthesis of ultrasmall Cu2O-coordinated carbon nitrides on ceria for multimodal antitumor therapy. Mater. Horiz., 2023,10, 1342-1353. https://doi.org/10.1039/D2MH01540D
Tris (4,7-diphenyl-1,10-phenanthroline) ruthenium (II)dichloride (Ru(dpp)3Cl2) was obtained from Maokang Biotechnology Co., Ltd. (Shanghai, China).
[6] Shixin Zhang et al. Photoresponsive metal-organic framework with combined photodynamic therapy and hypoxia-activated chemotherapy for the targeted treatment of rheumatoid arthritis. Colloids and Surfaces B: Biointerfaces. Volume 234, February 2024, 113707
[Ru(dpp) 3]Cl 2 was purchased from Shanghai Maokang Biotechnology Co. Ltd. (China).
[7] Shuanglong Tang et al. A near-infrared/tumor microenvironment dual-responsive cobalt-based material for photothermal/photodynamic/DNA-damaging chemotherapy synergistic therapy and anti-inflammation. Chemical Engineering Journal. Volume 488, 15 May 2024, 150906.
Singlet oxygen sensor green (SOSG) and tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(Ⅱ) dichloride {[Ru(dpp) 3]Cl 2} were obtained from MaokangBio, Shanghai, China.
[8]Wang T, Du M, Yuan Z, Guo J, Chen Z. Multi-functional nanosonosensitizer-engineered bacteria to overcome tumor hypoxia for enhanced sonodynamic therapy. Acta Biomater. 2024 Nov;189:519-531. doi: 10.1016/j.actbio.2024.10.013. Epub 2024 Oct 10. PMID: 39395706.
Ru(dpp) 3 Cl 2 was purchased from Maokang Biotechnology Co., Ltd. (Shanghai, China).
[9] Zhiyin Li et al. “Balloon-like” biomimetic erythrocyte vesicles potentiate photodynamic therapy for inducing immunogenic cell death. Chemical Engineering Journal. Volume 500, 15 November 2024, 157406 [Ru(dpp) 3]Cl 2 (36309–88-3) was bought from Shanghai Maokang Biotech (China) .
[10] Lijian Cao, et al. The direct catalytic synthesis of ultrasmall Cu2O-coordinated carbon nitrides on ceria for multimodal antitumor therapy. January 2023Materials Horizons 10(4) DOI:10.1039/D2MH01540D Tris (4,7-diphenyl-1,10-phenanthroline) ruthenium (II) dichloride (Ru(dpp)3Cl2)was obtained from Maokang Biotechnology Co., Ltd. (Shanghai, China).
[11]Wang D, Liao Y, Yan H, Zhu S, Liu Y, Li J, Wang X, Guo X, Gu Z, Sun B. In Situ Formed Z-Scheme Graphdiyne Heterojunction Realizes NIR-Photocatalytic Oxygen Evolution and Selective Radiosensitization for Hypoxic Tumors. ACS Nano. 2022 Dec 27;16(12):21186-21198. doi: 10.1021/acsnano.2c09169. Epub 2022 Nov 29. PMID: 36445074. The [Ru(dpp)3]Cl2 was obtained from MKbio (China).
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