【务必注意】：初次使用离子探针的用户，强烈建议配合：Pluronic F-127, Cell Culture Tested 细胞培养级（MS4301-1G）一起使用，以提高探针的水溶性和胞内加载性。 

产品标签

PBFI AM 钾离子指示剂/探，SBFI AM 钠离子指示剂/探针，Asante Potassium Green-2，Valinomycin 缬氨霉素，CAS NO：124549-23-1

产品信息: 现货供应，欢迎咨询。

产品描述

PBFI，英文全名Potassium-binding Benzofuran Isophthalate，一种K+敏感的荧光探针，用来测定细胞和细胞内区隔（Intracellular compartments）的K+水平变化。虽然PBFI对K+的选择能力弱于Ca2+指示剂比如Fura-2，但在其他一价阳离子存在体系中，PBFI足以检测K+的生理浓度。结合离子后的PBFI光谱变化可通过激发光比率测定来分析，其能与使用相同光滤片和仪器检测的探针Fura-2共同使用。

PBFI对K+的解离常数（Kd）非常依赖于Na+的存在与否。在不含Na+的体系，PBFI对K+的Kd值为5.1mM；而在含135mM K+/ Na+总浓度（约为生理离子强度）的溶液体系，PBFI对K+的Kd值为44mM；若缓冲液中的Na+用四甲基氯化铵所替代，PBFI对K+的Kd值变为11mM。氯化胆碱和N-甲基葡萄糖胺是培养基中另两种可能替代Na+的化合物。虽然PBFI对K+的选择性比Na+仅强1.5倍，这一选择能力已足以满足检测需求，因为正常情况细胞内K+浓度比Na+高10倍左右。

本品为乙酰氧基甲基酯（Acetoxymethyl ester, AM ester）形式的PBFI，CAS NO：124549-23-1，具有细胞膜渗透性，只需简单孵育即可进入细胞，常用加载浓度范围5-10µM，加载时间40min-4h，根据具体的实验要求和细胞类型来调整。

产品特性

1） 化学名：4,4'-[1,4,10,13-tetraoxa-7,16-diazacyclooctadecane-7,16-diylbis(5-methoxy-6,2-benzofurandiyl)]bis- 1,3-benzenedicarboxylic acid, tetrakis[(acetyloxy)methyl] ester

2） 同义名：Potassium-binding Benzofuran Isophthalate Acetoxymethyl ester

3） CAS NO：124549-23-1

4） 分子式：C58H62N2O24

5） 分子量：1171.1

6） 纯度：≥95%

7） Ex/Em：~340,380/500 nm

8） 外观：黄色至橙色粉末

9） 溶解性：溶于DMSO（10mM）和甲醇

10）化学结构式：

保存与运输方法

保存：-20ºC避光干燥保存，2年有效。

运输：冰袋运输。

注意事项

1） PBFI AM易受潮，粉末需干燥保存；粉末需用无水DMSO溶解，配制储存液（如10mM），置于-20℃干燥避光保存，小量分装避免反复冻融，至少3个月稳定。

2） PBFI AM由于水溶性较差，建议使用Pluronic F-127以优化探针的细胞加载效率。通常情况，将PBFI AM的DMSO储存液与等体积Pluronic F-127（25% w/v）混合均匀，之后即刻加入适量的细胞加载缓冲液（cell loading buffer）中达到所需浓度。

3） 为了您的安全和健康，请穿实验服并戴一次性手套操作。

 — —Written/Edited by V. Shallan【版权归MKBio懋康所有】

上海懋康生物科技有限公司是一家涉足于生命科学和生物技术领域研究的试剂、仪器和实验室消耗品与实验服务工作，主要从事细胞生物学、植物学、分子生物学、免疫学、生物化学、蛋白组学。生物制药与诊断试剂研发生产等领域。 本公司秉承“以人为本，以诚为信、合同守信”的经营理念。坚持"品质保障"的原则为广大客户提供优质产品。

文献引用：

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Tong W et al. Phthalocyanine functionalized poly(glycidyl methacrylate) nano-assemblies for photodynamic inactivation of bacteria. Biomater. Sci., 2019,7, 1905-1918

Sodium-binding benzofuran isophthalate acetoxymethyl ester (SBFI-AM) and Potassium-binding Benzofuran Isophthalate Acetoxymethyl ester (PBFI-AM) were purchased from MKbio. China.

[2]

Li R et al. Biofilm inhibition and mode of action of epigallocatechin gallate against Vibrio mimicus. Food Control, Volume 113, July 2020, 107148

Then PBFI probe (ShangHai MaoKang Bio technology Co., LTD., China) was added and incubated at 37 °C for 90 min. The cells were washed, collected and resuspended with PBS buffer. Aliquots (100 μL) of bacterial suspension were transferred to a Corning 96 well black plate, and 100 μL of various concentrations of EGCG were dispensed in the microtiter plate wells.

[3]

Liu Y, Zhen W, Wang Y, Song S, Zhang H. Na2S2O8 Nanoparticles Trigger Antitumor Immunotherapy through Reactive Oxygen Species Storm and Surge of Tumor Osmolarity. J Am Chem Soc. 2020 Dec 30;142(52):21751-21757. doi: 10.1021/jacs.0c09482. Epub 2020 Dec 18. PMID: 33337859.

4T1 cells were inoculated into glass bottom culture dishes for 24 h. Then, adding PNSO NPs medium solution (80 μg/mL) to continue co-culture for 4 h. The treated 4T1 cells were further incubated with 10

µM Na+ indicator SBFI AM (sodiumbinding benzofuran isophthalate acetoxymethyl ester, MKBio, 90%) in 0.04% Pluronic F-127 (MKBio) and the fluorescence signal was measured by CLSM.

[4] 

Liang Z, Yang Y, Yu G, et al. Engineering aluminum hydroxyphosphate nanoparticles with well-controlled surface property to enhance humoral immune responses as vaccine adjuvants. Biomaterials. 2021 Jun;275:120960. DOI: 10.1016/j.biomaterials.2021.120960.

[ 5]BMDMs were treated with AAHPs (250 μg/mL) in the presence of LPS at 500 ng/mL for 5 h. Then PBFI AM (Mkbio, Shanghai, China) was added to the cells at the concentration of 10 μM, and cells were incubated at 37 °C for 1h. Triton X-100 (0.2%) treated cells were used as controls. The fluorescence of PBFI AM was measured at the Ex/Em of 340/615 nm. The data were expressed as relative fluorescence intensity (RFI) defined as the fluorescence intensity of AAHPs-treated BMDMs normalized to the intensity of control cells.

[6] Jia Y, Yang B, Shi J, Fang D, Wang Z, Liu Y. Melatonin prevents conjugative transfer of plasmid-mediated antibiotic resistance genes by disrupting proton motive force. Pharmacol Res. 2022 Jan;175:105978. doi: 10.1016/j.phrs.2021.105978. Epub 2021 Nov 21. PMID: 34813930.

As for the detection of intracellular K + concentration, the PBFI-AM (K + indicator) fluorescence dye (Maokangbio, Shanghai, China) labeled cells (10 μM) in the presence of…..