产品简介:
2-Deoxy-D-glucose 2-脱氧-D-葡萄糖
产品标签
2-Deoxy-D-glucose (2-DG) 2-脱氧-D-葡萄糖；2-deoxyglucose；Hexokinase 己糖激酶；glycolysis糖酵解抑制剂； CAS：154-17-6
产品信息
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产品名称
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产品编号
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CAS NO.
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规格
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价格（元）
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2-Deoxy-D-glucose 2-脱氧-D-葡萄糖
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MS0871-0001G
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154-17-6
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1g
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200
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2-Deoxy-D-glucose 2-脱氧-D-葡萄糖
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MS0871-0005G
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154-17-6
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5g
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500
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2-Deoxy-D-glucose 2-脱氧-D-葡萄糖
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MS0871-0025G
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154-17-6
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25g
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1900
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产品描述
2-脱氧-D-葡萄糖（2-Deoxy-D-glucose, 2-DG），一种不可代谢的葡萄糖类似物，通过作用于己糖激酶（糖酵解的限速步骤）来抑制糖酵解。2-DG被己糖激酶磷酸化生成2-DG-P，后者不能被磷酸葡萄糖异构酶进一步代谢，进而引起2-DG-P在细胞内的累积和细胞内ATP的耗尽。体外缺氧模型中，2-DG导致细胞周期抑制和细胞死亡，诱导自噬，增加ROS生成和活化AMPK。体内动物模型中阻断肿瘤细胞生长。
产品特性
1） CAS NO：154-17-6
2） 化学名：(4R,5S,6R)-6-(Hydroxymethyl)oxane-2,4,5-triol
3） 同义名：2-deoxyglucose; 2-Deoxy-D-arabinohexose; D-Arabino-2-deoxyhexose; Ba 2758; NSC 15193; 2-脱氧葡萄糖；2-去氧-D-葡萄糖；D-2-脱氧葡萄糖；
4） 分子式：C6H12O5
5） 分子量：164.16
6） 外观：白色至类白色固体
7） 纯度：>98%
8） 溶解性：溶于DMSO（20mg/ml）、H2O（30mg/ml）、PBS, pH 7.2 （10mg/ml）
9）化学结构式：
保存与运输方法
保存：2-8℃避光干燥保存，至少2年有效。
运输：室温运输。
注意事项
1） 本品储存液置于≤-20℃避光保存，尽量避免反复冻融。通常情况下，-20℃至少一个月稳定，-80℃至少6个月稳定。
2） 本品仅用作科研用途，绝不可用于临床诊断或治疗药物，绝不可用做食品或药品。
3） 为了您的安全和健康，请穿实验服并戴一次性手套操作。
储存液配制
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 质量
 溶剂体积
浓度
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1mg
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5mg
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10mg
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1mM
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6.0916 mL
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30.4581 mL
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60.9162mL
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5mM
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1.2183mL
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6.0916 mL
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12.1832mL
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10mM
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0.6092 mL
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3.0458 mL
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6.0916 mL
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50mM
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0.1218 mL
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0.6092 mL
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1.2183 mL
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使用方法【源自文献，仅作参考】
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文献1，Yan J et al. The bifunctional autophagic flux by 2-deoxyglucose to control survival or growth of prostate cancer cells. Cancer Res. 2005 Aug 1;65(15):7023-30. PMID: 16061689
体外研究（细胞实验）：
细胞类型（Cell types）：MCF-7 cells
实验方法（Assay）：For cell growth assay, MCF-7 cells were plated at 3 × 104 cells per well in flat-bottomed 96-well plates in 100 μL of culture medium. After 24 hours, cells were fed with fresh medium including 2-DG at doses of 0, 4, 8, or 16 mmol/L. At days 1, 3, and 5 after 2-DG exposure, cells were fixed with 1% glutaraldehyde, replaced with PBS, and stored at 4°C. At the end of an experiment, all of the plates were stained with 0.02% aqueous crystal violet for 30 minutes and rinsed with deionized water. After redissolving the bound crystal violet in 70% ethanol, the absorbance was determined at 590 nm.
实验结果（Result）：Cell growth was significantly inhibited by 2-DG at doses of 4, 8, or 16 mmol/L for 1, 3, or 5 days in a dose- and time-dependent manner (P < 0.001).
体内研究（动物模型）：
动物模型（Animal Model）：At 21 days of age, rats were injected with 50 mg 1-methyl-1-nitrosourea per kilogram of body weight (i.p.). Six days following carcinogen injection, all rats were randomized into one of three groups, 30 rats per group, and werefed ad libitum AIN-93G diet containing0.0%, 0.02%, or 0.03% (w/w) 2-DGfor 5 weeks.
实验结果（Result）：2-DG decreased the incidence and multiplicity of mammary carcinomas and prolonged cancer latency (P < 0.05). The 0.02% dose of 2-DG had no effect on circulating levels of glucose, insulin, insulin-like growth factor-I, IGF binding protein-3, leptin, or body weight gain.
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文献2，Wang H et al. 2-deoxy-D-glucose enhances anesthetic effects in mice. Anesth Analg. 2015 Feb; 120(2):312-9. PMID: 25390277
体内研究（动物模型）：
动物模型（Animal Model）：Female Sprague-Dawley rats at 20 days of age
配制方法（Formulation）：2-DGwasdiluted insaline.
实验方法（Assay）：2-DG (1000 mg/kg), 3-NP (50 mg/kg),30 or saline was injected [intraperitoneally (ip)] 5 minutes before isoflurane anesthesia.Idebenone (200 mg/kg) or L-carnitine (100 mg/kg) was injected 15 minutes before isoflurane anesthesia (10 minutes before treatment with 2-DG or 3-NP).
实验结果（Result）：2-DG enhances isoflurane-induced loss of righting reflex (LORR) in mice.
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— —Written/Edited by V. Shallan【版权归MKBio懋康所有】
上海懋康生物科技有限公司是一家涉足于生命科学和生物技术领域研究的试剂、仪器和实验室消耗品与实验服务工作，主要从事细胞生物学、植物学、分子生物学、免疫学、生物化学、蛋白组学。生物制药与诊断试剂研发生产等领域。 本公司秉承“以人为本，以诚为信、合同守信”的经营理念。坚持"品质保障"的原则为广大客户提供优质产品。
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