目录号 | MF3604-2UG | 售价 | 1000.00元 | ||||||||||||||||||||||||||||||
规格 | 2μg | 运输温度 | 冰袋运输 | ||||||||||||||||||||||||||||||
其他名称 | pGADT7 AD Vector | 保存温度 | -20℃保存, | ||||||||||||||||||||||||||||||
CAS号 | N/A | 有效期 | 1年 | ||||||||||||||||||||||||||||||
应用 | 酵母双杂交 | 订购数量 |
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产品简介: pGADT7 AD Vector酵母双杂交克隆载体
搜索关键词: pGADT7 AD; pGBKT7 Vector;pGADT7-T;pGBKT7-Lam;SD/-Trp;Clontech;Yeast two-hybrid (Y2H)酵母双杂交;
产品订购:更大包装或产品技术问题,请来电/在线咨询,电话:021-54736159 。网站:www.maokangbio.com。
基本信息
质粒图谱
描述:pGADT7 AD是一种酵母双杂交表达载体,特别设计用来表达介于GAL4 AD(a.a. 768-881)和目的蛋白之间的融合蛋白。在ADH1全长启动子的驱动下高水平表达融合蛋白。融合蛋白含一个HA表位标签。为了便于体外转录/翻译,在GAL4 AD和HA表位标签之间又引入T7启动子。
保存与运输方法 保存:-20℃保存,至少1年有效。 运输:冰袋运输。
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质粒序列 LOCUS Exported 7987 bp ds-DNA circular SYN 09-SEP-2012 DEFINITION Yeast two-hybrid ""prey"" vector for expressing proteins fused to the GAL4 activation domain. KEYWORDS pGADT7 AD SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7987) AUTHORS Clontech TITLE Direct Submission http://www.miaolingbio.com FEATURES Location/Qualifiers source 1..7987 /organism="synthetic DNA construct" /lab_host="Saccharomyces cerevisiae" /mol_type="other DNA" promoter 771..1475 /gene="S. cerevisiae ADH1" /note="ADH1 promoter" /note="promoter for alcohol dehydrogenase 1" CDS 1491..1493 /codon_start=1 /product="start codon for expression in yeast" /note="ATG" /translation="M" CDS 1521..1541 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /note="SV40 NLS" /translation="PKKKRKV" CDS 1557..1898 /codon_start=1 /gene="Saccharomyces cerevisiae GAL4 (truncated)" /product="activation domain of the GAL4 transcriptional activator" /note="GAL4 activation domain" /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD EDTPPNPKKE" promoter 1904..1922 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" CDS 1935..1937 /codon_start=1 /product="start codon for in vitro transcription/translation" /note="ATG" /translation="M" CDS 1941..1967 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /note="HA" /translation="YPYDVPDYA" misc_feature 1968..2035 /label=MCS /note="MCS" /note="multiple cloning site" terminator 2415..2602 /gene="S. cerevisiae ADH1" /note="ADH1 terminator" /note="transcription terminator for alcohol dehydrogenase 1" CDS complement(2719..3813) /codon_start=1 /gene="S. cerevisiae LEU2" /product="3-isopropylmalate dehydrogenase, required for leucine biosynthesis" /note="LEU2" /note="yeast auxotrophic marker" /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL IGGAAIEATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG DLGGSNSTTEVGDAVAEEVKKILA" promoter complement(3814..4219) /gene="S. cerevisiae LEU2" /note="LEU2 promoter" primer_bind complement(4261..4277) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" protein_bind 4285..4301 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4309..4339) /note="lac promoter" /note="promoter for the E. coli lac operon" rep_origin complement(4814..5402) /direction=LEFT /note="ori" /note="high-copy-number colE1/pMB1/pBR322/pUC origin of replication" CDS complement(5573..6433) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(6434..6538) /gene="bla" /note="AmpR promoter" rep_origin 6820..7984 /note="2u ori" /note="yeast 2u plasmid origin of replication" ORIGIN 1 tgcatgcctg caggtcgaga tccgggatcg aagaaatgat ggtaaatgaa ataggaaatc 61 aaggagcatg aaggcaaaag acaaatataa gggtcgaacg aaaaataaag tgaaaagtgt 121 tgatatgatg tatttggctt tgcggcgccg aaaaaacgag tttacgcaat tgcacaatca 181 tgctgactct gtggcggacc cgcgctcttg ccggcccggc gataacgctg ggcgtgaggc 241 tgtgcccggc ggagtttttt gcgcctgcat tttccaaggt ttaccctgcg ctaaggggcg 301 agattggaga agcaataaga atgccggttg gggttgcgat gatgacgacc acgacaactg 361 gtgtcattat ttaagttgcc gaaagaacct gagtgcattt gcaacatgag tatactagaa 421 gaatgagcca agacttgcga gacgcgagtt tgccggtggt gcgaacaata gagcgaccat 481 gaccttgaag gtgagacgcg cataaccgct agagtacttt gaagaggaaa cagcaatagg 541 gttgctacca gtataaatag acaggtacat acaacactgg aaatggttgt ctgtttgagt 601 acgctttcaa ttcatttggg tgtgcacttt attatgttac aatatggaag ggaactttac 661 acttctccta tgcacatata ttaattaaag tccaatgcta gtagagaagg ggggtaacac 721 ccctccgcgc tcttttccga tttttttcta aaccgtggaa tatttcggat atccttttgt 781 tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca tacatcggga 841 ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga gatatacaat 901 agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt acactgcctc 961 attgatggtg gtacataacg aactaatact gtagccctag acttgatagc catcatcata 1021 tcgaagtttc actacccttt ttccatttgc catctattga agtaataata ggcgcatgca 1081 acttcttttc tttttttttc ttttctctct cccccgttgt tgtctcacca tatccgcaat 1141 gacaaaaaaa tgatggaaga cactaaagga aaaaattaac gacaaagaca gcaccaacag 1201 atgtcgttgt tccagagctg atgaggggta tctcgaagca cacgaaactt tttccttcct 1261 tcattcacgc acactactct ctaatgagca acggtatacg gccttccttc cagttacttg 1321 aatttgaaat aaaaaaaagt ttgctgtctt gctatcaagt ataaatagac ctgcaattat 1381 taatcttttg tttcctcgtc attgttctcg ttccctttct tccttgtttc tttttctgca 1441 caatatttca agctatacca agcatacaat caactccaag ctttgcaaag atggataaag 1501 cggaattaat tcccgagcct ccaaaaaaga agagaaaggt cgaattgggt accgccgcca 1561 attttaatca aagtgggaat attgctgata gctcattgtc cttcactttc actaacagta 1621 gcaacggtcc gaacctcata acaactcaaa caaattctca agcgctttca caaccaattg 1681 cctcctctaa cgttcatgat aacttcatga ataatgaaat cacggctagt aaaattgatg 1741 atggtaataa ttcaaaacca ctgtcacctg gttggacgga ccaaactgcg tataacgcgt 1801 ttggaatcac tacagggatg tttaatacca ctacaatgga tgatgtatat aactatctat 1861 tcgatgatga agatacccca ccaaacccaa aaaaagagat ctttaatacg actcactata 1921 gggcgagcgc cgccatggag tacccatacg acgtaccaga ttacgctcat atggccatgg 1981 aggccagtga attccacccg ggtgggcatc gatacgggat ccatcgagct cgagctgcag 2041 atgaatcgta gatactgaaa aaccccgcaa gttcacttca actgtgcatc gtgcaccatc 2101 tcaatttctt tcatttatac atcgttttgc cttcttttat gtaactatac tcctctaagt 2161 ttcaatcttg gccatgtaac ctctgatcta tagaattttt taaatgacta gaattaatgc 2221 ccatcttttt tttggaccta aattcttcat gaaaatatat tacgagggct tattcagaag 2281 ctttggactt cttcgccaga ggtttggtca agtctccaat caaggttgtc ggcttgtcta 2341 ccttgccaga aatttacgaa aagatggaaa agggtcaaat cgttggtaga tacgttgttg 2401 acacttctaa ataagcgaat ttcttatgat ttatgatttt tattattaaa taagttataa 2461 aaaaaataag tgtatacaaa ttttaaagtg actcttaggt tttaaaacga aaattcttat 2521 tcttgagtaa ctctttcctg taggtcaggt tgctttctca ggtatagcat gaggtcgctc 2581 ttattgacca cacctctacc ggccggtcga aattccccta ccctatgaac atattccatt 2641 ttgtaatttc gtgtcgtttc tattatgaat ttcatttata aagtttatgt acaaatatca 2701 taaaaaaaga gaatcttttt aagcaaggat tttcttaact tcttcggcga cagcatcacc 2761 gacttcggtg gtactgttgg aaccacctaa atcaccagtt ctgatacctg catccaaaac 2821 ctttttaact gcatcttcaa tggccttacc ttcttcaggc aagttcaatg acaatttcaa 2881 catcattgca gcagacaaga tagtggcgat agggttgacc ttattctttg gcaaatctgg 2941 agcagaaccg tggcatggtt cgtacaaacc aaatgcggtg ttcttgtctg gcaaagaggc 3001 caaggacgca gatggcaaca aacccaagga acctgggata acggaggctt catcggagat 3061 gatatcacca aacatgttgc tggtgattat aataccattt aggtgggttg ggttcttaac 3121 taggatcatg gcggcagaat caatcaattg atgttgaacc ttcaatgtag gaaattcgtt 3181 cttgatggtt tcctccacag tttttctcca taatcttgaa gaggccaaaa cattagcttt 3241 atccaaggac caaataggca atggtggctc atgttgtagg gccatgaaag cggccattct 3301 tgtgattctt tgcacttctg gaacggtgta ttgttcacta tcccaagcga caccatcacc 3361 atcgtcttcc tttctcttac caaagtaaat acctcccact aattctctga caacaacgaa 3421 gtcagtacct ttagcaaatt gtggcttgat tggagataag tctaaaagag agtcggatgc 3481 aaagttacat ggtcttaagt tggcgtacaa ttgaagttct ttacggattt ttagtaaacc 3541 ttgttcaggt ctaacactac ctgtacccca tttaggacca cccacagcac ctaacaaaac 3601 ggcatcagcc ttcttggagg cttccagcgc ctcatctgga agtgggacac ctgtagcttc 3661 gatagcagca ccaccaatta aatgattttc gaaatcgaac ttgacattgg aacgaacatc 3721 agaaatagct ttaagaacct taatggcttc ggctgtgatt tcttgaccaa cgtggtcacc 3781 tggcaaaacg acgatcttct taggggcaga cattagaatg gtatatcctt gaaatatata 3841 tatatattgc tgaaatgtaa aaggtaagaa aagttagaaa gtaagacgat tgctaaccac 3901 ctattggaaa aaacaatagg tccttaaata atattgtcaa cttcaagtat tgtgatgcaa 3961 gcatttagtc atgaacgctt ctctattcta tatgaaaagc cggttccggc gctctcacct 4021 ttcctttttc tcccaatttt tcagttgaaa aaggtatatg cgtcaggcga cctctgaaat 4081 taacaaaaaa tttccagtca tcgaatttga ttctgtgcga tagcgcccct gtgtgttctc 4141 gttatgttga ggaaaaaaat aatggttgct aagagattcg aactcttgca tcttacgata 4201 cctgagtatt cccacagttg ggggatctcg actctagcta gaggatcaat tcgtaatcat 4261 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 4321 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 4381 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctga taacttcgta 4441 taatgtatgc tatacgaagt tattaggtct gaagaggagt ttacgtccag ccaagctagc 4501 ttggctgcag gtcgagcggc cgcgatccgg aacccttaat ataacttcgt ataatgtatg 4561 ctatacgaag ttatcagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 4621 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 4681 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 4741 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 4801 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 4861 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 4921 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 4981 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 5041 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 5101 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 5161 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 5221 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc 5281 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 5341 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 5401 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 5461 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 5521 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 5581 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 5641 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 5701 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 5761 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 5821 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 5881 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 5941 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 6001 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 6061 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 6121 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 6181 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 6241 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 6301 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 6361 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 6421 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 6481 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 6541 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct 6601 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa 6661 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga 6721 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact 6781 atgcggcatc agagcagatt gtactgagag tgcaccataa cgcatttaag cataaacacg 6841 cactatgccg ttcttctcat gtatatatat atacaggcaa cacgcagata taggtgcgac 6901 gtgaacagtg agctgtatgt gcgcagctcg cgttgcattt tcggaagcgc tcgttttcgg 6961 aaacgctttg aagttcctat tccgaagttc ctattctcta gctagaaagt ataggaactt 7021 cagagcgctt ttgaaaacca aaagcgctct gaagacgcac tttcaaaaaa ccaaaaacgc 7081 accggactgt aacgagctac taaaatattg cgaataccgc ttccacaaac attgctcaaa 7141 agtatctctt tgctatatat ctctgtgcta tatccctata taacctaccc atccaccttt 7201 cgctccttga acttgcatct aaactcgacc tctacatttt ttatgtttat ctctagtatt 7261 actctttaga caaaaaaatt gtagtaagaa ctattcatag agtgaatcga aaacaatacg 7321 aaaatgtaaa catttcctat acgtagtata tagagacaaa atagaagaaa ccgttcataa 7381 ttttctgacc aatgaagaat catcaacgct atcactttct gttcacaaag tatgcgcaat 7441 ccacatcggt atagaatata atcggggatg cctttatctt gaaaaaatgc acccgcagct 7501 tcgctagtaa tcagtaaacg cgggaagtgg agtcaggctt tttttatgga agagaaaata 7561 gacaccaaag tagccttctt ctaaccttaa cggacctaca gtgcaaaaag ttatcaagag 7621 actgcattat agagcgcaca aaggagaaaa aaagtaatct aagatgcttt gttagaaaaa 7681 tagcgctctc gggatgcatt tttgtagaac aaaaaagaag tatagattct ttgttggtaa 7741 aatagcgctc tcgcgttgca tttctgttct gtaaaaatgc agctcagatt ctttgtttga 7801 aaaattagcg ctctcgcgtt gcatttttgt tttacaaaaa tgaagcacag attcttcgtt 7861 ggtaaaatag cgctttcgcg ttgcatttct gttctgtaaa aatgcagctc agattctttg 7921 tttgaaaaat tagcgctctc gcgttgcatt tttgttctac aaaatgaagc acagatgctt 7981 cgttgct //
— —Written/Edited by V. Shallan【版权归MKBio懋康所有】
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