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当前位置:首页> 新闻资讯> 新品发布 >Nourseothricin (NTC) Sulfate 硫酸诺尔斯菌素 CAS:96736-11-7 【筛选抗生素】
Nourseothricin (NTC) Sulfate 硫酸诺尔斯菌素 CAS:96736-11-7 【筛选抗生素】
时间:2016-04-24     作者:懋康生物     文章来源:原创作品    

搜索关键词:

链丝菌素 Streptothricin;clonNAT;选择性抗生素;诺尔丝菌素;革兰氏阳性和阴性菌;酵母菌和丝状真菌;植物;sat1基因;nat1基因;CAS:96736-11-7

 

基本描述:

诺尔斯菌素(Nourseothricin,缩写NTC),英文同义名:Streptothricin和clonNAT,由诺尔斯氏链霉菌( Streptomyces noursei)代谢产生的一种广谱型抗生素,因结构上具链丝菌素(Streptothricin,STs)化学基团,故归属于此类抗生素亚家族。天然产物提取的诺尔斯菌素(Nourseothricin)一般为ST-F和ST-D的混合物,具有极其宽广的抑制生长活性,原核生物如细菌;真核生物如包括白色念球菌在内的各种酵母菌、丝状真菌、原生生物、昆虫和植物【见下表格1】。诺尔斯菌素的作用机制是通过诱导mRNA错误编码,来抑制蛋白合成。

基于以上特性,诺尔斯菌素(Nourseothricin)是基因重组工程研究中非常优秀的选择性抗生素。抗性基因nat1最初从S. noursei分离所得,此基因能编码表达诺尔斯菌素N-乙酰转移酶,通过对诺尔斯菌素(NTC)上与糖基部分相连的ß-赖氨酸上的ß-氨基基团进行单乙酰化修饰,使得抗生素失活。研究发现,nat1基因在多种异质体系内发挥作用,使其在分子遗传学中是非常有价值的选择性工具。后面陆续发现具相同抗性机理的基因有:来自大肠杆菌E.Coli的基因stat1,stat2,stat3;来自链霉菌S. lavendulae的stat基因。另外,还有来自链霉菌S. albulus的新型sttH基因,作用机制不同,抗性在于编码表达异分支酸类型水解酶,能打开streptolidine内酰胺环上的酰胺键,将其转化成无活性的酸性产物。sttH基因抗性似乎只对酵母菌有活性,对细菌无效。

 

诺尔斯菌素(Nourseothricin,缩写NTC)具有以下应用优势:

1)粉末或者液体的稳定性非常高。粉末+4℃保存10年有效,+20℃保存2年有效。

2)粉末具非常高的水溶性(1g/L);

3)具非常低或几乎无背景抗性:因抗性蛋白仅在细胞内表达,不会在细胞培养基内被降解。

4)不会与大量其他抗生素,如氨基糖苷类抗生素潮霉素B或遗传霉素G418发生交叉反应。

5)不能用作诊疗抗生素,对临床上治疗的细菌微生物呈现出极弱抗性。因此与诊疗抗生素交叉反应很小;

6)特别适用于酵母分子生物学研究。不像一些营养缺陷型选择性基因,诺尔斯菌素抗性不会影响酵母正常生长,能用于工程菌,或者缺少营养性筛选基因表达的野生菌。


 

诺尔斯菌素(Nourseothricin,缩写NTC)使用方法和推荐浓度:

储存液配制:称量1000mg诺尔斯菌素溶解于5ml去离子水充分溶解后,用0.22µm滤膜过滤除菌,即制备200mg/ml储存液,本溶液置于+4℃保存,至少4周稳定,不会有任何可见的活力损失。长期保存,分装冻存于-20℃.

建议筛选浓度:诺尔斯菌素在相对较低的浓度下即可有效用于重组工程菌的筛选,大多数有机体的筛选浓度多在50-200µg/ml区间内。 具体可参考下表1。

 

产品订购信息:【日本进口分装,现货供应

货号                      

产品名称                                                               

规格            

价格(元)   

货期            

MS0026-20MG

Nourseothricin (NTC) Sulfate 硫酸诺尔斯菌素

20mg

396

现货

MS0026-100MG

Nourseothricin (NTC) Sulfate 硫酸诺尔斯菌素

100mg

1056

现货

MS0026-500MG

Nourseothricin (NTC) Sulfate 硫酸诺尔斯菌素

500mg

4186

现货

 

另提供美国Cayman公司,原装进口的Nourseothricin (NTC) Sulfate 硫酸诺尔斯菌素,CAS:96736-11-7,货期:3-4周

货号                     

产品名称                                                                

规格            

价格(元)   

品牌            

16227

Nourseothricin (NTC) Sulfate 硫酸诺尔斯菌素

1mg

350

Cayman

16227

Nourseothricin (NTC) Sulfate 硫酸诺尔斯菌素

5mg

700

Cayman

16227

Nourseothricin (NTC) Sulfate 硫酸诺尔斯菌素

10mg

1232

Cayman

 

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MS0010-1G

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Puromycin Dihydrochloride 嘌呤霉素盐酸盐

100mg

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MS0011-500MG

Puromycin Dihydrochloride 嘌呤霉素盐酸盐

500mg

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表1诺尔斯菌素(NTC)最低抑制浓度和选择性浓度列表

分类                                

物种                                                           

MIC(µg/ml)    

筛选浓度(µg/ml)  

Gram-negative bacteria

革兰氏阳性菌

Agrobacterium tumefaciens  


100

Escherichia coli

2–12

50

Francisella tularensis


50

Pseudomonas aeruginosa

50

100

Gram-positive bacteria

革兰氏阴性菌

Bacillus subtilis

5

50

Enterococcus faecium

8-256

500

Staphylococcus aureus

2–12

50

Streptomycetes 链霉菌

Streptomyces lividans

6

100

Yeast 酵母菌

Candida albicans

200

250– 450

Hansenula polymorpha


100

Kluyveromyces lactis


50

Pichia pastoris


100

Saccharomyces cerevisiae

25

75-100

Schizosaccharomyces pombe

40

100

Other Ascomycota

其它子囊菌

Acremonium chrysogenum


25

Aspergillus nidulans


120

Cryphonectria parasitica


100

Neurospora crassa


200

Penicillium chrysogenum


150-200

Podospora anserina


50

Sordaria macrospora


50

Trichophyton mentagrophytes


50

Basidiomycota 担子菌

Cryptococcus neoformans


100

Schizophyllum commune

3

8

Ustilago maydi


75-100

Protozoa 原生动物

Leishmania tarentolae, major etc.


100

Phytomonasserpens


100

Plasmodium falciparum

75**


Toxoplasma gondii


500

Microalgae 微藻

Phaeodactylum tricornutum


50-250

Thalassiosira pseudonana


100

Plants 植物

Arabidopsis thaliana

20

50-200

Daucus carota


100

Lotus corniculatus


50

Nicotiana tabacum


100

Oryza sativa

20

200

* MIC: Minimal inhibitory concentration ** IC50: Concentration inhibiting growth by 50%


参考文献:

Abbott et al. (2013) Overcoming recalcitrant transformation and gene manipulation in Pucciniomycotina yeasts. Appl. Microbiol. Biotechnol. 97: 283

Alshahni et al. (2010) Nourseothricin acetyltransferase: a new dominant selectable marker for the dermatophyte Trichophyton mentagrophytes. Medical Mycology 48: 665

Ben-Daniel et al. (2012) Pectate lyase affects pathogenicity in natural isolates of Colletotrichum coccodes and in pelA genedisrupted and gene-overexpressing mutant lines. Molecular Plant Biology 13: 187

Branduardi et al. (2014) Molecular Tools and Protocols for Engineering the Acid-Tolerant Yeast Zygosaccharomyces bailii as a Potential Cell Factory. Methods in Molecular Biology 1152: 63

Buhmann et al. (2014) A Tyrosine-Rich Cell Surface Protein in the Diatom Amphora coffeaeformis Identified through Transcriptome Analysis and Genetic Transformation. PLOS one 9: e110369 Calvey et al. (2014) An optimized transformation protocol for Lipomyces starkeyi. Current Genetics 60: 223

Cho et al. (2012) Transcription factor Amr1 induces melanin biosynthesis and suppresses virulence in Alternaria brassicicola. PLoS Pathology 8: e1002974

Cox et al. (2003) Superoxide Dismutase Influences the Virulence of Cryptococcus neoformans by Affecting Growth within Macrophages. Infect. Immunity 71: 173

Djouani-Tahri et al. (2011) A eukaryotic LOV-histidine kinase with circadian clock function in the picoalga Ostreococcus. Plant Journal 65: 578

Dubey et al. (2013) Role of the methylcitrate cycle in growth, antagonism and induction of systemic defence responses in the fungal biocontrol agent Trichoderma atroviride. Microbiology 159: 2492 9

Dubey et al. (2014) Hydrophobins are required for conidial hydrophobicity and plant root colonization in the fungal biocontrol agent Clonostachys rosea. BMC Microbiology 14:18 El-Khoury et al. (2008) Gene deletion and allelic replacement in the filamentous fungus Podospora anserina. Curr. Genet. 53: 249

Gassel et al. 2014 Genetic engineering of the complete carotenoid pathway towards enhanced astaxanthin formation in Xanthophyllomyces dendrorhous starting from a high-yield mutant. Appl. Microbiol. Biotechnol. 98: 345

Giner-Lamia et al. (2015) CopM is a novel copper-binding protein involved in copper resistance in Synechocystis sp. PCC 6803. MicrobiologyOpen 4:167

Goarin et al. (2015) Gene replacement in Penicillium roqueforti. Current Genetics 61: 203

Gold et al. (1994) Three selectable markers for transformation of Ustilago maydis. Gene 142: 225 Goldstein et al. (1999) Three New Dominant Drug Resistance Cassettes for Gene Disruption in Saccharomyces cerevisiae. Yeast 15: 1541

Goyard et al. 2014 In vivo imaging of trypanosomes for a better assessment of host–parasite relationships and drug efficacy. Parasitol. Int. 63: 260–268

Hamano et al. (2006) A Novel Enzyme Conferring Streptothricin Resistance Alters the Toxicity of Streptothricin D from Broadspectrum to Bacteria-specific. J. Biol. Chem. 281: 16842

Hentges et al. (2005) Three novel antibiotic marker cassettes for gene disruption and marker switching in Schizosaccharomyces pombe. Yeast 22: 1013



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