alamarBlue® protocol: Method to determine LD50 using alamarBlue
alamarBlue can be used to determine the median lethal dose LD50 value and similar assays testing the effects of a toxin, radiation, or pathogen against cell cultures.
|
Percentage difference in reduction (equation 5) |
= |
A LW - ( A HW x RO ) for test well A LW - ( A HW x RO ) for control well |
x100
|
|
Use semi-log graph paper to plot the percent of untreated control (using equation 5) for each dilution of a given test agent on the y-axis vs. the concentration of the test agent on the x-axis.
To determine the LD50 endpoint from the graph, read from where the 50 percent point intercepts the Dose.
Response Curve to the concentration along the x-axis. This concentration is the LD50 value.
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产品名称 |
产品编号 |
规格 |
价格（元） |
货期 |
|
Alamar Blue 阿尔玛蓝细胞增殖及毒性检测试剂 |
MX3006-5ML |
5ml (500T) |
295 |
现货 |
|
Alamar Blue 阿尔玛蓝细胞增殖及毒性检测试剂 |
MX3006-10ML |
10ml (1000T) |
550 |
现货 |
|
Alamar Blue 阿尔玛蓝细胞增殖及毒性检测试剂 |
MX3006-50ML |
50ml (5000T) |
1925 |
现货 |
