EAE动物模型建立之：

MOG (35-55), Mouse, Rat 大小鼠髓鞘少突胶质细胞糖蛋白（35-55）

产品关键词：

MOG (35-55) 髓鞘少突胶质细胞糖蛋白（35-55）；中枢神经系统（CNS）；Multiple Sclerosis (MS) 多发性硬化症；实验性自身免疫性脑脊髓炎（EAE）；PLP (178-191)；MBP (87-99)；CAS：149635-73-4

产品描述

MOG，英文全名Myelin oligodendrocyte glycoprotein，中文名髓鞘少突胶质细胞糖蛋白，髓鞘的一种微量成分，属于免疫球蛋白超家族成员之一。也是特定表达于中枢神经系统（CNS）的自身抗原，诱导多发性硬化症的原发性脱髓鞘特征。

MOG (35-55)是髓鞘少突胶质细胞糖蛋白（Myelin Oligodendrocyte Glycoprotein, MOG）的一个片段，能够诱导啮齿类动物自身免疫抗体生成，进而产生复发-缓解型神经性疾病，表现出大量斑块状脱髓鞘病症。MOG (35-55)诱导的实验性自身免疫性脑脊髓炎（EAE）是用来研究多发性硬化症（MS）的一种重要模型。

[This 21 amino acid peptide is a fragment of Myelin Oligodendrocyte Glycoprotein (MOG) that induces autoantibody production, which in turn produces a relapsing-remitting neurologic disease with extensive plaque-like demyelination in rodents.  This MOG (35-55) induced experimental autoimmune encephalomyelitis serve as a model to study Multiple Sclerosis (MS).]

产品特性

1）   CAS NO：149635-73-4 [net]

2）  同义名：Myelin Oligodendrocyte Glycoprotein Peptide Fragment 35-55 Rat, Mouse;大小鼠MOG（35-55）；

3）   分子式：C118H177N35O29S

4）   分子量：2581.96 g/mol

5）   纯度：≥98%（HPLC）

6）   外观：白色冻干粉

7）   溶解性：溶于水（0.5-1 mg/ml）

8）   单字母序列：MEVGWYRSPFSRVVHLYRNGK

9）   三字母序列：Met-Glu-Val-Gly-Trp-Tyr-Arg-Ser-Pro-Phe-Ser-Arg-Val-Val-His-Leu-Tyr-Arg-Asn-Gly-Lys

产品信息【来自美国CSBio的MOG35-55，产品不仅高纯且含有高含量的活性多肽≥80%，是质量保证的重要参数。广泛应用于建立EAE动物模型，有大量的应用文献支持。目前我司懋康生物有做备货，大大缩减你的货期，以及降低采购成本。本品有特价，欢迎来电咨询，021-54736159，QQ:2971634497】

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引用文献【由于内容太多，以下文献列表简化书写，请根据文章名称自行搜索】

Bhlhe40 controls cytokine production by T cells and is essential for pathogenicity in autoimmune neuroinflammation. PMID: 24699451. [For active EAE induction, mice were immunized subcutaneously with 100 μg MOG(35-55) (C S Bio Co.) emulsified in CFA (made with 5 mg/ml heat killed M. tuberculosis H37Ra (BD Difco) in incomplete Freund’s adjuvant (BD Difco)). Pertussis toxin (List Biological)]

Treatment of Experimental Autoimmune Encephalomyelitis by Codelivery of Disease Associated Peptide and Dexamethasone in Acetalated Dextran Microparticles. PMID: 24433027 [Mice were immunized with a complete Freund’s adjuvant (CFA) and MOG peptide emulsion along with pertussis toxin, purchased from Hooke Laboratories (Lawrence, MS), per the manufacturer’s suggestions. After immunization, mice were given clinical scores as previously described.MOG35–55 peptide was purchased from CS Bio]

Lymph node-derived donor encephalitogenic CD4+ T cells in C57BL/6 mice adoptive transfer experimental autoimmune encephalomyelitis highly express GM-CSF and T-bet. PMID: 21702922 [Donor mice were anesthetized with tribromoethanol (®Avertin; Sigma Aldrich, St. Louis, MO) 250 mg/kg intraperitoneally (i.p.) and then immunized subcutaneously (s.c.) with a homogenized emulsion of 200 μg/100 μL MOGp35-55 (C.S. Bio)/Complete Freund's Adjuvant (CFA) supplemented with 2 mg/ml of mycobacterium tuberculosis H37RA (MT) (DIFCO Laboratories). 25 μl of emulsion was injected into the bilateral scapular and inguinal areas. In some experiments, the donor mice or the recipient mice were also injected i.p. with 200 ng/200 μL pertussis toxin (PT) (List Biological Laboratories Inc)/phosphate buffered saline (PBS) on the day of immunization and 2 days post immunization.]

The neonatal CNS is not conducive for encephalitogenic Th1 T cells and B cells during experimental autoimmune encephalomyelitis. PMID: 23705890 [Mouse myelin oligodendrocyte glycoprotein peptide (MOGp) 35–55 [MEVGWYRSPFSRVVHLYRNGK] was synthesized by CS Bio.To induce active EAE, C57BL/6 female mice were immunized subcutaneously with MOGp35-55 emulsified in an equal volume of complete Freund adjuvant (CFA) (DIFCO Laboratories, Detroit, MI, USA) in each flank. Mice were 4 days, 1, 2, 3, 4, 5, 6, 7, 8, and 20 weeks of age. Immediately after the immunization, and again 48 hours later, mice received an intravenous injection of pertussis toxin (Ptx) in PBS. All animals received an equivalent dose of Ag, adjuvants, and Ptx on a dose per weight basis: Per 20 g bodyweight, 100 μl of vaccine, containing 100 μg MOGp35-55 and 2 mg/ml mycobacterium, as well as 400 ng Ptx were administered.]

Macrophage Migration Inhibitory Factor Potentiates Autoimmune-Mediated Neuroinflammation. PMID: 23797673 [MOG35–55 (MEVGWYRSPFSRVVHLYRNGK) was purchased from C S Bio and was purified by HPLC (purity >95%).C57BL/6 WT and MIF−/− mice were immunized s.c. over four sites on each of the flanks, with 0.1 ml of an emulsion containing 200 μg MOG35–55 in PBS mixed with an equal volume of CFA containing 200 μg heat-killed Mycobacterium tuberculosis, Jamaica strain.]

Defining standard enzymatic dissociation methods for individual brains and spinal cords in EAE. PMID: 29359175 [Mouse myelin oligodendrocyte glycoprotein35–55 (MOGp35–55) (MEVGWYRSPFSRVVHLYRNGK) was synthesized by CS Bio (Menlo Park, CA).Briefly, 8–12 week old male C57BL/6 mice were anesthetized with tribromoethanol (Avertin; Sigma-Aldrich, St. Louis, MO) 250 mg/kg intraperitoneally (i.p.), and subsequently immunized subcutaneously with MOGp35–55 (100 μg/mouse), emulsified in an equal volume of complete Freund adjuvant14 containing 4 mg/mL H37Ra Mycobacterium tuberculosis (Difco; BD, Franklin Lakes, NJ) in each flank. At the time of immunization and 48 hours later, mice received an i.p. injection of 200 ng pertussis toxin in 200 μL PBS. For all experiments, individual animals were observed daily based on the EAE clinical scoring system as follows: 0 = no clinical disease, 1 = loss of tail tone, 2 = mild paraparesis, 3 = paraplegia, 4 = hindlimb and forelimb paralysis, and 5 = moribund or death.]

Lineage-Specific Metabolic Properties and Vulnerabilities of T Cells in the Demyelinating Central Nervous System. PMID: 28507026 [Briefly, male or female (sex-matched within experiments) C57BL/6 mice were immunized subcutaneously with 50 μg of myelin oligodendrocyte glycoprotein peptide 35–55 (MOG35–55) (CSBIO) emulsified at a 1:1 ratio in Complete Freud’s Adjuvant. On days 0 and 2, 250 ng of pertussis toxin (List Biologicals #180) was administered intraperitoneally. Transfer EAE was performed based on previously described protocols.]

ShcA regulates late stages of T cell development and peripheral CD4+ T cell numbers. PMID: 25595778 [For optimal EAE induction, 10-wk-old female mice were immunized s.c. into the lower back with 100 μg myelin oligodendrocyte gp35–55 [MOG(35–55)] peptide (CS Bio), emulsified in an equal volume of CFA (Sigma-Aldrich) supplemented with heat-killed Mycobacterium tuberculosis (clone H37RA; Difco), for a total of 400 μg H37RA/mouse. Mice received 200 ng pertussis toxin (List Biologicals) i.p. on days 0 and 1 after immunization. For suboptimal EAE induction, mice were immunized s.c. with 75 μg MOG(35–55) in CFA supplemented with H37RA but received only a single i.p. injection of 200 ng pertussis toxin on day 0.]

IL-1-induced Bhlhe40 identifies pathogenic T helper cells in a model of autoimmune neuroinflammation. PMID: 26834156. [For EAE induction, mice were immunized subcutaneously with 100 µg MOG35–55 (CS Bio Co.) emulsified in CFA (made with 5 mg/ml heat-killed Mtb H37Ra [BD] in incomplete Freund’s adjuvant [BD]). PTX (List Biological Laboratories) or mutant PTX (mPTX; mutated at two positions in the S1 subunit [R9K and E129A]; List Biological Laboratories) was injected i.p. (300 ng) on days 0 and 2. Mice were monitored for signs of classical EAE for at least 28 d and graded on a standard 0–5 scale, as previously described.]

Repulsive guidance molecule-a is involved in Th17-cell-induced neurodegeneration in autoimmune encephalomyelitis. PMID: 25456136

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