FD NeuroTechnologies, Inc.;Golgi-Cox impregnation 浸染法;Degenerating neurons 退化神经元;Fluoro-Jade B;Fluoro-Jade C;
产品描述:
FD NeuroTechnologies, Inc(USA)公司生产的FD退化神经元银染试剂盒(FD NeuroSilver™ Kit II)特别开发用于实验动物中枢神经系统(CNS)固定组织切片内的退化神经元检测。该试剂盒检测原理基于发现正衰退神经元上的一些元件比如溶酶体、轴突和末梢出现特别的嗜银性。在某些特定条件下,这些细胞元件高亲和性结合银离子。一旦还原后,银离子形成光学或电子显微镜下可见的金属颗粒。
FD退化神经元银染试剂盒(FD NeuroSilver™ Kit II)广泛用在各种实验条件下的动物研究中。该试剂盒经证实高度特异性检测大脑和脊髓内衰退神经元胞体、轴突和末梢,且灵敏度非常高。该试剂盒还适合用来检测少量衰退神经元,特别是那些用传统组织病理染色技术未检测到的。
FD退化神经元银染试剂盒(FD NeuroSilver™ Kit II)还被证实能检测转基因小鼠大脑的淀粉样斑块(amyloid plaques),结果非常灵敏且可靠。还可用于免疫组化组织切片的神经衰退和/或淀粉样斑块检测。本试剂盒整个操作流程约需1h。
试剂盒组分(室温运输和保存):
|
|
PK301A(Small) |
PK301A(large) |
1 |
Solution A 溶液A |
250 ml |
500 ml |
2 |
Solution B 溶液B |
250 ml |
500 ml |
3 |
Solution C 溶液C |
250 ml x 2 |
500 ml l x 2 |
4 |
Solution D 溶液D |
250 ml |
500 ml |
5 |
Solution E 溶液E |
1 ml |
2 ml |
6 |
Solution F 溶液F |
2 ml |
3 ml |
7 |
Solution G (5X) 溶液G (5X) |
250 ml |
500 ml |
8 |
Glass Specimen Retriever |
2 |
2 |
9 |
Natural hair paintbrush |
1 |
1 |
10 |
User Manual 使用手册 |
1 |
1 |
应用实例:
图1. 大鼠中风模型大脑神经衰退的检测 Detection of neurodegeneration in the rat brain of a stroke model. 40 µm cryostat section was cut coronally through the striatum of a rat used as an animal model of stroke. The section was processed for demonstrating neuronal damage with FD NeuroSilver™ Kit. Note the accumulation of metallic silver grains (black) in both the striatum and the cortex, indicating the presence of neurodegeneration.
图2. 小鼠阿尔茨海默病模型大脑衰退性神经元和淀粉样斑块的检测Detection of neurodegeneration and amyloid plaques in the mouse brain of a model for Alzeimer’s disease. 40 µm cryostat section was cut coronally through the dorsal hippocampus of a transgenic mouse used as a model for studing Alzeimer’s disease. The section was processed for detecting both neuronal damage and amyloid plaques with FD NeuroSilver™ Kit. Note silver-stained plaques in both the hippocampus and the cortex.
图3. 大鼠亨廷顿疾病模型大脑衰退性神经元的检测 Detection of neurodegeneration in the rat brain of a model for Huntington’s disease. 40 µm cryostat section was cut coronally through the striatum of a rat that received a focal injection of quinolinic acid. The section was processed first for bcl2-immunoreactivity and then for detecting neuronal damage with FD NeuroSilver™ Kit. Note that degenerating neurons (black) intermingle with survived bcl2-positive neurons.
订购信息:原装正品,欢迎来电咨询。电话:021-54736159,18121428569。QQ:3308240863,2971634497。网址:www.maokangbio.com
货号 |
名称 |
规格 |
价格(元) |
PK301 |
FD NeuroSilver™ Kit II (large) 退化神经元银染试剂盒 |
For 300 sections |
7194 |
PK301A |
FD NeuroSilver™ Kit II (small) 退化神经元银染试剂盒 |
For 150 sections |
5457 |
近200篇使用文献的数据支持,是值得信赖的科研辅助精品。详见操作手册。
PK301 FD NeuroSilver™ Kit User Mannual
— —Written/Edited by V. Shallan【版权归MKBio懋康所有】
上海懋康生物科技有限公司是一家涉足于生命科学和生物技术领域研究的试剂、仪器和实验室消耗品与实验服务工作,主要从事细胞生物学、植物学、分子生物学、免疫学、生物化学、蛋白组学。生物制药与诊断试剂研发生产等领域。 本公司秉承“以人为本,以诚为信、合同守信”的经营理念。坚持"品质保障"的原则为广大客户提供优质产品。