质粒序列
LOCUS Exported 7804 bp ds-DNA circular 05-JUL-2019
COMMENT Created by Takara Bio USA, Inc. http://www.takarabio.com
COMMENT Cat. Nos. 630466, 630489, 630498, 630499
SOURCE synthetic DNA construct
FEATURES Location/Qualifiers
source 1..7804
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 456..1160
/gene="S. cerevisiae ADH1"
CDS 1188..1628
/label=GAL4 DNA binding domain
promoter 1639..1657
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 1677..1706
/label=Myc tag
misc_feature 1740..2244
/label=Human Lamin A/C
terminator 2265..2312
/label=T7 terminator
terminator 2339..2526
/gene="S. cerevisiae ADH1"
/label=ADH1 terminator
rep_origin 2952..3540
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
polyA_signal 3869..3916
/label=HSV TK poly(A) signal
CDS complement(4148..4942)
/label=NeoR/KanR
promoter complement(4943..5047)
/label=AmpR promoter
rep_origin 5074..6416
/label=2u ori
/note="yeast 2u plasmid origin of replication"
promoter 6675..6956
/label=TRP1 promoter
CDS 6957..7631
/label=TRP1
rep_origin complement(join(7761..7804,1..385))
/label=f1 ori
ORIGIN
1 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
61 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
121 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
181 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
241 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
301 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
361 cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gctgcgcaac tgttgggaag
421 ggcgatcggt gcgggcctct tcgctattac gccagatcct tttgttgttt ccgggtgtac
481 aatatggact tcctcttttc tggcaaccaa acccatacat cgggattcct ataatacctt
541 cgttggtctc cctaacatgt aggtggcgga ggggagatat acaatagaac agataccaga
601 caagacataa tgggctaaac aagactacac caattacact gcctcattga tggtggtaca
661 taacgaacta atactgtagc cctagacttg atagccatca tcatatcgaa gtttcactac
721 cctttttcca tttgccatct attgaagtaa taataggcgc atgcaacttc ttttcttttt
781 ttttcttttc tctctccccc gttgttgtct caccatatcc gcaatgacaa aaaaatgatg
841 gaagacacta aaggaaaaaa ttaacgacaa agacagcacc aacagatgtc gttgttccag
901 agctgatgag gggtatctcg aagcacacga aactttttcc ttccttcatt cacgcacact
961 actctctaat gagcaacggt atacggcctt ccttccagtt acttgaattt gaaataaaaa
1021 aaagtttgct gtcttgctat caagtataaa tagacctgca attattaatc ttttgtttcc
1081 tcgtcattgt tctcgttccc tttcttcctt gtttcttttt ctgcacaata tttcaagcta
1141 taccaagcat acaatcaact ccaagcttga agcaagcctc ctgaaagatg aagctactgt
1201 cttctatcga acaagcatgc gatatttgcc gacttaaaaa gctcaagtgc tccaaagaaa
1261 aaccgaagtg cgccaagtgt ctgaagaaca actgggagtg tcgctactct cccaaaacca
1321 aaaggtctcc gctgactagg gcacatctga cagaagtgga atcaaggcta gaaagactgg
1381 aacagctatt tctactgatt tttcctcgag aagaccttga catgattttg aaaatggatt
1441 ctttacagga tataaaagca ttgttaacag gattatttgt acaagataat gtgaataaag
1501 atgccgtcac agatagattg gcttcagtgg agactgatat gcctctaaca ttgagacagc
1561 atagaataag tgcgacatca tcatcggaag agagtagtaa caaaggtcaa agacagttga
1621 ctgtatcgcc ggaatttgta atacgactca ctatagggcg agccgccatc atggaggagc
1681 agaagctgat ctcagaggag gacctgcata tggccatgga ggccgaattc ccggggatct
1741 ctgaagaggt ggtcagccgc gaggtgtccg gcatcaaggc cgcctacgag gccgagctcg
1801 gggatgcccg caagaccctt gactcagtag ccaaggagcg cgcccgcctg cagctggagc
1861 tgagcaaagt gcgtgaggag tttaaggagc tgaaagcgcg caataccaag aaggagggtg
1921 acctgatagc tgctcaggct cggctgaagg acctggaggc tctgctgaac tccaaggagg
1981 ccgcactgag cactgctctc agtgagaagc gcacgctgga gggcgagctg catgatctgc
2041 ggggccaggt ggccaagctt gaggcagccc taggtgaggc caagaagcaa cttcaggatg
2101 agatgctgcg gcgggtggat gctgagaaca ggctgcagac catgaaggag gaactggact
2161 tccagaagaa catctacagt gaggagctgc gtgagaccaa gcgccgtcat gagacccgac
2221 tggtggagat tgacaggatc cgtcgacctg cagcggccgc ataactagca taaccccttg
2281 gggcctctaa acgggtcttg aggggttttt tgcgcgcttg cagccaagct aattccgggc
2341 gaatttctta tgatttatga tttttattat taaataagtt ataaaaaaaa taagtgtata
2401 caaattttaa agtgactctt aggttttaaa acgaaaattc ttattcttga gtaactcttt
2461 cctgtaggtc aggttgcttt ctcaggtata gcatgaggtc gctcttattg accacacctc
2521 taccggcatg caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
2581 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
2641 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
2701 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
2761 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
2821 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
2881 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
2941 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
3001 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
3061 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
3121 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
3181 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
3241 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
3301 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
3361 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
3421 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
3481 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
3541 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
3601 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
3661 tgaagtttta aatcaatcta aagtatatat gagtaacctg aggctatggc agggcctgcc
3721 gccccgacgt tggctgcgag ccctgggcct tcacccgaac ttggggggtg gggtggggaa
3781 aaggaagaaa cgcgggcgta ttggccccaa tggggtctcg gtggggtatc gacagagtgc
3841 cagccctggg accgaacccc gcgtttatga acaaacgacc caacaccgtg cgttttattc
3901 tgtcttttta ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt ccgtgtttca
3961 gttagcctcc ccctagggtg ggcgaagaac tccagcatga gatccccgcg ctggaggatc
4021 atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa ggcggcggtg
4081 gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc gaaccccaga
4141 gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag
4201 cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa
4261 tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt
4321 cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat
4381 gagtcacgac gagatcctcg ccgtcgggca tgctcgcctt gagcctggcg aacagttcgg
4441 ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca
4501 tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg
4561 gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag
4621 caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc
4681 ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg
4741 atagccgcgc tgcctcgtct tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa
4801 aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg
4861 tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt
4921 gcaatccatc ttgttcaatc atactcttcc tttttcaata ttattgaagc atttatcagg
4981 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
5041 ttccgcgcac atttccccga aaagtgccac ctgaacgaag catctgtgct tcattttgta
5101 gaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt
5161 acagaacaga aatgcaacgc gaaagcgcta ttttaccaac gaagaatctg tgcttcattt
5221 ttgtaaaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca
5281 tttttacaga acagaaatgc aacgcgagag cgctatttta ccaacaaaga atctatactt
5341 cttttttgtt ctacaaaaat gcatcccgag agcgctattt ttctaacaaa gcatcttaga
5401 ttactttttt tctcctttgt gcgctctata atgcagtctc ttgataactt tttgcactgt
5461 aggtccgtta aggttagaag aaggctactt tggtgtctat tttctcttcc ataaaaaaag
5521 cctgactcca cttcccgcgt ttactgatta ctagcgaagc tgcgggtgca ttttttcaag
5581 ataaaggcat ccccgattat attctatacc gatgtggatt gcgcatactt tgtgaacaga
5641 aagtgatagc gttgatgatt cttcattggt cagaaaatta tgaacggttt cttctatttt
5701 gtctctatat actacgtata ggaaatgttt acattttcgt attgttttcg attcactcta
5761 tgaatagttc ttactacaat ttttttgtct aaagagtaat actagagata aacataaaaa
5821 atgtagaggt cgagtttaga tgcaagttca aggagcgaaa ggtggatggg taggttatat
5881 agggatatag cacagagata tatagcaaag agatactttt gagcaatgtt tgtggaagcg
5941 gtattcgcaa tattttagta gctcgttaca gtccggtgcg tttttggttt tttgaaagtg
6001 cgtcttcaga gcgcttttgg ttttcaaaag cgctctgaag ttcctatact ttctagagaa
6061 taggaacttc ggaataggaa cttcaaagcg tttccgaaaa cgagcgcttc cgaaaatgca
6121 acgcgagctg cgcacataca gctcactgtt cacgtcgcac ctatatctgc gtgttgcctg
6181 tatatatata tacatgagaa gaacggcata gtgcgtgttt atgcttaaat gcgtacttat
6241 atgcgtctat ttatgtagga tgaaaggtag tctagtacct cctgtgatat tatcccattc
6301 catgcggggt atcgtatgct tccttcagca ctacccttta gctgttctat atgctgccac
6361 tcctcaattg gattagtctc atccttcaat gctatcattt cctttgatat tggatcatac
6421 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
6481 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
6541 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
6601 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
6661 gtgcaccata gatcaacgac attactatat atataatata ggaagcattt aatagaacag
6721 catcgtaata tatgtgtact ttgcagttat gacgccagat ggcagtagtg gaagatattc
6781 tttattgaaa aatagcttgt caccttacgt acaatcttga tccggagctt ttcttttttt
6841 gccgattaag aattaattcg gtcgaaaaaa gaaaaggaga gggccaagag ggagggcatt
6901 ggtgactatt gagcacgtga gtatacgtga ttaagcacac aaaggcagct tggagtatgt
6961 ctgttattaa tttcacaggt agttctggtc cattggtgaa agtttgcggc ttgcagagca
7021 cagaggccgc agaatgtgct ctagattccg atgctgactt gctgggtatt atatgtgtgc
7081 ccaatagaaa gagaacaatt gacccggtta ttgcaaggaa aatttcaagt cttgtaaaag
7141 catataaaaa tagttcaggc actccgaaat acttggttgg cgtgtttcgt aatcaaccta
7201 aggaggatgt tttggctctg gtcaatgatt acggcattga tatcgtccaa ctgcatggag
7261 atgagtcgtg gcaagaatac caagagttcc tcggtttgcc agttattaaa agactcgtat
7321 ttccaaaaga ctgcaacata ctactcagtg cagcttcaca gaaacctcat tcgtttattc
7381 ccttgtttga ttcagaagca ggtgggacag gtgaactttt ggattggaac tcgatttctg
7441 actgggttgg aaggcaagag agccccgaaa gcttacattt tatgttagct ggtggactga
7501 cgccagaaaa tgttggtgat gcgcttagat taaatggcgt tattggtgtt gatgtaagcg
7561 gaggtgtgga gacaaatggt gtaaaagact ctaacaaaat agcaaatttc gtcaaaaatg
7621 ctaagaaata ggttattact gagtagtatt tatttaagta ttgtttgtgc acttgccgat
7681 ctatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt
7741 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
7801 ccaa
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