| 目录号 | MF3602-2UG | 售价 | 1000.00元 | ||||||||||||||||||||||||||||
| 规格 | 2μg | 运输温度 | 冰袋运输 | ||||||||||||||||||||||||||||
| 其他名称 | pGBKT7-53 Control vector | 保存温度 | -20℃保存 | ||||||||||||||||||||||||||||
| CAS号 | N/A | 有效期 | 1年 | ||||||||||||||||||||||||||||
| 应用 | 酵母双杂交 | 订购数量 |
|
||||||||||||||||||||||||||||
|
产品简介: pGBKT7-53 Vector 酵母双杂交对照载体
搜索关键词： pGBKT7-53 Vector；pGADT7-T；pGBKT7-Lam；SD/-Trp；Clontech；Yeast two-hybrid (Y2H)酵母双杂交；
产品订购：更大包装或产品技术问题，请来电/在线咨询，电话：021-54736159 。网站：www.maokangbio.com。
基本信息
质粒图谱
描述： pGBKT7-53是阳性对照质粒，能编码小鼠p53蛋白（a.a.72-390）和GAL4 DNA-BD（a.a.1-147）的融合蛋白。小鼠p53 cDNA克隆进入pGBKT7的EcoRI和BamH1位点之间。p53 cDNA来源于Lwabuchi et al.（1993）年文献提到的质粒（由Clontech改造所得）。
保存与运输方法 保存：-20℃保存，至少1年有效。 运输：冰袋运输。
注意事项
质粒序列 LOCUS Exported 8274 bp ds-DNA circular 05-JUL-2019 COMMENT Created by Takara Bio USA, Inc. http://www.takarabio.com COMMENT Cat. Nos. 630466, 630489, 630498, 630499 SOURCE synthetic DNA construct FEATURES Location/Qualifiers source 1..8274 CDS 642..1082 /gene="Saccharomyces cerevisiae GAL4 (truncated)" /product="DNA binding domain of the GAL4 transcriptional activator" /label=GAL4 DNA binding domain promoter 1093..1111 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" misc_feature 1176..2141 /label=amino acids 72-390 of mouse p53 protein terminator 2189..2236 /label=T7 terminator polymerase" terminator 2263..2450 /gene="S. cerevisiae ADH1" /label=ADH1 terminator alcohol dehydrogenase 1 (ADH1) gene" rep_origin 2876..3464 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" polyA_signal 3793..3840 /label=HSV TK poly(A) signal CDS complement(4072..4866) /label=NeoR/KanR promoter complement(4867..4971) /gene="bla" /label=AmpR promoter rep_origin 4998..6340 /label=2 u ori /note="yeast 2u plasmid origin of replication" promoter 6599..6880 /gene="S. cerevisiae TRP1" /label=TRP1 promoter CDS 6881..7555 /codon_start=1 /label=TRP1 /note="yeast auxotrophic marker" rep_origin complement(7658..8113) /label=f1 ori promoter join(8184..8274,1..614) /gene="S. cerevisiae ADH1" /label=ADH1 promoter ORIGIN 1 tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac cagacaagac 61 ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg tacataacga 121 actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca ctaccctttt 181 tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct ttttttttct 241 tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaat gatggaagac 301 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga 361 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactctc 421 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaaagtt 481 tgctgtcttg ctatcaagta taaatagacc tgcaattatt aatcttttgt ttcctcgtca 541 ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa gctataccaa 601 gcatacaatc aactccaagc ttgaagcaag cctcctgaaa gatgaagcta ctgtcttcta 661 tcgaacaagc atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga 721 agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt 781 ctccgctgac tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc 841 tatttctact gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac 901 aggatataaa agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg 961 tcacagatag attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa 1021 taagtgcgac atcatcatcg gaagagagta gtaacaaagg tcaaagacag ttgactgtat 1081 cgccggaatt tgtaatacga ctcactatag ggcgagccgc catcatggag gagcagaagc 1141 tgatctcaga ggaggacctg catatggcca tggaggccga attccctgtc accgagaccc 1201 ctgggccagt ggcccctgcc ccagccactc catggcccct gtcatctttt gtcccttctc 1261 aaaaaactta ccagggcaac tatggcttcc acctgggctt cctgcagtct gggacagcca 1321 agtctgttat gtgcacgtac tctcctcccc tcaataagct attctgccag ctggcgaaga 1381 cgtgccctgt gcagttgtgg gtcagcgcca cacctccagc tgggagccgt gtccgcgcca 1441 tggccatcta caagaagtca cagcacatga cggaggtcgt gagacgctgc ccccaccatg 1501 agcgctgctc cgatggtgat ggcctggctc ctccccagca tcttatccgg gtggaaggaa 1561 atttgtatcc cgagtatctg gaagacaggc agacttttcg ccacagcgtg gtggtacctt 1621 atgagccacc cgaggccggc tctgagtata ccaccatcca ctacaagtac atgtgtaata 1681 gctcctgcat ggggggcatg aaccgccgac ctatccttac catcatcaca ctggaagact 1741 ccagtgggaa ccttctggga cgggacagct ttgaggttcg tgtttgtgcc tgccctggga 1801 gagaccgccg tacagaagaa gaaaatttcc gcaaaaagga agtcctttgc cctgaactgc 1861 ccccagggag cgcaaagaga gcgctgccca cctgcacaag cgcctctccc ccgcaaaaga 1921 aaaaaccact tgatggagag tatttcaccc tcaagatccg cgggcgtaaa cgcttcgaga 1981 tgttccggga gctgaatgag gccttagagt taaaggatgc ccatgctaca gaggagtctg 2041 gagacagcag ggctcactcc agctacctga agaccaagaa gggccagtct acttcccgcc 2101 ataaaaaaac aatggtcaag aaagtggggc ctgactcaga ctgactgcct ctgcatcccg 2161 gatccgtcga cctgcagcgg ccgcataact agcataaccc cttggggcct ctaaacgggt 2221 cttgaggggt tttttgcgcg cttgcagcca agctaattcc gggcgaattt cttatgattt 2281 atgattttta ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac 2341 tcttaggttt taaaacgaaa attcttattc ttgagtaact ctttcctgta ggtcaggttg 2401 ctttctcagg tatagcatga ggtcgctctt attgaccaca cctctaccgg catgcaagct 2461 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 2521 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 2581 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 2641 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 2701 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 2761 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 2821 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 2881 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 2941 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 3001 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 3061 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 3121 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 3181 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 3241 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 3301 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 3361 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 3421 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 3481 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 3541 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 3601 tctaaagtat atatgagtaa cctgaggcta tggcagggcc tgccgccccg acgttggctg 3661 cgagccctgg gccttcaccc gaacttgggg ggtggggtgg ggaaaaggaa gaaacgcggg 3721 cgtattggcc ccaatggggt ctcggtgggg tatcgacaga gtgccagccc tgggaccgaa 3781 ccccgcgttt atgaacaaac gacccaacac cgtgcgtttt attctgtctt tttattgccg 3841 tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt ttcagttagc ctccccctag 3901 ggtgggcgaa gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc 3961 ggaaaacgat tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgtg 4021 atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc cagagtcccg ctcagaagaa 4081 ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag 4141 cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa 4201 cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa 4261 gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgagtca cgacgagatc 4321 ctcgccgtcg ggcatgctcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg 4381 atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg 4441 ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag 4501 ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag 4561 gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac 4621 gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc 4681 gtcttgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc 4741 ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc 4801 atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc 4861 aatcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 4921 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 4981 ccgaaaagtg ccacctgaac gaagcatctg tgcttcattt tgtagaacaa aaatgcaacg 5041 cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca 5101 acgcgaaagc gctattttac caacgaagaa tctgtgcttc atttttgtaa aacaaaaatg 5161 caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa 5221 atgcaacgcg agagcgctat tttaccaaca aagaatctat acttcttttt tgttctacaa 5281 aaatgcatcc cgagagcgct atttttctaa caaagcatct tagattactt tttttctcct 5341 ttgtgcgctc tataatgcag tctcttgata actttttgca ctgtaggtcc gttaaggtta 5401 gaagaaggct actttggtgt ctattttctc ttccataaaa aaagcctgac tccacttccc 5461 gcgtttactg attactagcg aagctgcggg tgcatttttt caagataaag gcatccccga 5521 ttatattcta taccgatgtg gattgcgcat actttgtgaa cagaaagtga tagcgttgat 5581 gattcttcat tggtcagaaa attatgaacg gtttcttcta ttttgtctct atatactacg 5641 tataggaaat gtttacattt tcgtattgtt ttcgattcac tctatgaata gttcttacta 5701 caattttttt gtctaaagag taatactaga gataaacata aaaaatgtag aggtcgagtt 5761 tagatgcaag ttcaaggagc gaaaggtgga tgggtaggtt atatagggat atagcacaga 5821 gatatatagc aaagagatac ttttgagcaa tgtttgtgga agcggtattc gcaatatttt 5881 agtagctcgt tacagtccgg tgcgtttttg gttttttgaa agtgcgtctt cagagcgctt 5941 ttggttttca aaagcgctct gaagttccta tactttctag agaataggaa cttcggaata 6001 ggaacttcaa agcgtttccg aaaacgagcg cttccgaaaa tgcaacgcga gctgcgcaca 6061 tacagctcac tgttcacgtc gcacctatat ctgcgtgttg cctgtatata tatatacatg 6121 agaagaacgg catagtgcgt gtttatgctt aaatgcgtac ttatatgcgt ctatttatgt 6181 aggatgaaag gtagtctagt acctcctgtg atattatccc attccatgcg gggtatcgta 6241 tgcttccttc agcactaccc tttagctgtt ctatatgctg ccactcctca attggattag 6301 tctcatcctt caatgctatc atttcctttg atattggatc atactaagaa accattatta 6361 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg 6421 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt 6481 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc 6541 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catagatcaa 6601 cgacattact atatatataa tataggaagc atttaataga acagcatcgt aatatatgtg 6661 tactttgcag ttatgacgcc agatggcagt agtggaagat attctttatt gaaaaatagc 6721 ttgtcacctt acgtacaatc ttgatccgga gcttttcttt ttttgccgat taagaattaa 6781 ttcggtcgaa aaaagaaaag gagagggcca agagggaggg cattggtgac tattgagcac 6841 gtgagtatac gtgattaagc acacaaaggc agcttggagt atgtctgtta ttaatttcac 6901 aggtagttct ggtccattgg tgaaagtttg cggcttgcag agcacagagg ccgcagaatg 6961 tgctctagat tccgatgctg acttgctggg tattatatgt gtgcccaata gaaagagaac 7021 aattgacccg gttattgcaa ggaaaatttc aagtcttgta aaagcatata aaaatagttc 7081 aggcactccg aaatacttgg ttggcgtgtt tcgtaatcaa cctaaggagg atgttttggc 7141 tctggtcaat gattacggca ttgatatcgt ccaactgcat ggagatgagt cgtggcaaga 7201 ataccaagag ttcctcggtt tgccagttat taaaagactc gtatttccaa aagactgcaa 7261 catactactc agtgcagctt cacagaaacc tcattcgttt attcccttgt ttgattcaga 7321 agcaggtggg acaggtgaac ttttggattg gaactcgatt tctgactggg ttggaaggca 7381 agagagcccc gaaagcttac attttatgtt agctggtgga ctgacgccag aaaatgttgg 7441 tgatgcgctt agattaaatg gcgttattgg tgttgatgta agcggaggtg tggagacaaa 7501 tggtgtaaaa gactctaaca aaatagcaaa tttcgtcaaa aatgctaaga aataggttat 7561 tactgagtag tatttattta agtattgttt gtgcacttgc cgatctatgc ggtgtgaaat 7621 accgcacaga tgcgtaagga gaaaataccg catcaggaaa ttgtaagcgt taatattttg 7681 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc 7741 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt 7801 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc 7861 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg 7921 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga 7981 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg 8041 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg 8101 ctacagggcg cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc 8161 gggcctcttc gctattacgc cagatccttt tgttgtttcc gggtgtacaa tatggacttc 8221 ctcttttctg gcaaccaaac ccatacatcg ggattcctat aataccttcg ttgg //
— —Written/Edited by V. Shallan【版权MKBio懋康所有】
上海懋康生物科技有限公司是一家涉足于生命科学和生物技术领域研究的试剂、仪器和实验室消耗品与实验服务工作，主要从事细胞生物学、植物学、分子生物学、免疫学、生物化学、蛋白组学。生物制药与诊断试剂研发生产等领域。 本公司秉承“以人为本，以诚为信、合同守信”的经营理念。坚持"品质保障"的原则为广大客户提供优质产品。 |
|||||||||||||||||||||||||||||||
