Agarose bound Lycopersicon Esculentum (Tomato) Lectin (LEL, TL)

AL-1173琼脂糖结合番茄凝集素

搜索关键词：

番茄凝集素；血管内灌注；凝集素灌注；微血管；内皮细胞；LEA；TL-1176 ;  VECTOR;  LEL,TL;  LAB

产品订购：【VECTOR】原装正品，现货供应，欢迎来电咨询，电话：021-54736159，QQ2971634497 ,1563822900.。

【温馨提示：见我司懋康生物整理的血管功能研究标志物-番茄凝集素产品专题】

产品描述：

番茄凝集素（Tomato lectin, Lycopersicon Esculentum lectin (LEL)）,是一种非常稳定的单亚基糖蛋白，约含有等量的蛋白和碳水化合物。据报道其糖类部分由85%半乳糖组成，在溶液中可能形成多聚体聚合物。番茄凝集素虽然和土豆凝集素、曼陀罗凝集素（DSL）和麦胚凝集素（WGA）有部分相同的特异性、但在许多多面呈现出独特之处。番茄凝集素LEL能够很好地与血型糖蛋白（glycophorin）和tamm-Horsfall糖蛋白结合，能够有效结合和标记啮齿类动物的血管内皮（vascularendothelium）

保存与运输方法：

保存：2-8℃干燥保存、有效期一年

运输：冰袋运输。

Overview

Features:

Matrix is heat stable, cross-linked 4% agarose beads with a molecular exlusion of about 2x107 daltons

Bead diameter ranges in size from 45-165 microns

Matrix is stable in solutions at pH 3-11 as well as many organic solvents

Immobilized lectins are prepared using affinity purified lectins

Covalent attachment preserves lectin activity and minimizes conformational changes that might result in nonspecific or hydrophobic interactions

Hydrophilic spacer arm is inserted between the lectin and the matrix

Conjugated proteins are not leached off the beads by Tris or other routinely used buffers

No residual charges present after conjugation.  This minimizes non-specific binding to the matrix

Product supplied as a 1:1 suspension in buffer

2 mg lectin/ml gel

Inhibiting/Eluting Sugar: Chitin Hydrolysate or Glycoprotein Eluting Solution (ES-5100)

More Information

Tomato lectin is a very stable single subunit glycoprotein containing about 50 percent arabinose and galactose and may form multimeric aggregates in solution. Tomato lectin, although sharing some specificities with potato lectin, Datura lectin, and wheat germ agglutinin, has been reported to be dissimilar in many respects. LEL binds well to glycophorin and Tamm-Horsfall glycoprotein and has been used effectively to label vascular endothelium in rodents.

Agarose bound* tomato lectin is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x107 daltons are used as the solid-phase matrix to which the lectins are covalently coupled. The attachment of the lectins to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins that might result in nonspecific ionic or hydrophobic interactions. The technique we have developed to couple lectins to agarose beads inserts a hydrophilic spacer arm between the lectin and the matrix.

This coupling method provides several advantages over the traditional cyanogen bromide procedure:

Maximum carbohydrate binding activity of the coupled lectins is retained

Linkage is stable over a range of pH values

Conjugated proteins are not leached off the beads by Tris or other routinely used buffers

No residual charges are present after conjugation. This minimizes non-specific binding to the matrix.

Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins.

Inhibiting/Eluting Sugar: Chitin Hydrolysate

*2 mg lectin/ml gel

注意事项：

为了您的安全和健康，请穿实验服并戴一次性手套操作。

另我司懋康生物提供自主品牌MKbio的番茄凝集素产品，质量保证，现货供应，欢迎来电咨询，电话：021-54736159，QQ2971634497 ,1563822900.。

 — —Written/Edited by V. Shallan【版权归MKBio懋康所有】

上海懋康生物科技有限公司是一家涉足于生命科学和生物技术领域研究的试剂、仪器和实验室消耗品与实验服务工作，主要从事细胞生物学、植物学、分子生物学、免疫学、生物化学、蛋白组学。生物制药与诊断试剂研发生产等领域。 本公司秉承“以人为本，以诚为信、合同守信”的经营理念。坚持"品质保障"的原则为广大客户提供优质产品。