FITC-Con A (FITC labeled Concanavalin A)

FITC标记刀豆蛋白A

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产品标签

淋巴细胞转化实验（T Lymphocyte Transformation Test），非特异性有丝分裂原（mitogen），T细胞活化和增殖，Con A，Concanavalin A ，伴刀豆凝集素，植物血球凝集素PHA，CAS：11028-71-0

产品信息

植物凝集素系列相关产品：

1）  见我司懋康生物整理的植物血凝素PHA（PHA-L，PHA-M，PHA-E，PHA-P）产品专题和信息。

2）  见我司懋康生物整理的番茄凝集素Tomato-Lectin产品专题和信息。

3）  见我司懋康生物整理的加纳籽凝集素I-同工凝集素B4（GSL I-B4，BSL I-B4）产品专题和信息。

4）  见我司懋康生物整理的刀豆蛋白凝集素Concanavalin A产品专题和信息。

5）  更多凝集素产品逐渐开发中。。。。。。

产品描述

刀豆蛋白A（Concanavalin A，Con A），来源于Canavalia ensiformis洋刀豆（刀豆），是一种凝集素蛋白（Mw 104kDa）。pH≥7.0条件下，Con A以同质四聚体结构形式存在，由四个分子量为26kDa的亚基构成。酸性条件（pH 4.5-5.5）下，Con A解离为活化的二聚体结构（52kDa）。每一个单体，不管pH值或分子结构如何，都包含2个金属离子结合位点。金属离子（Ca2+ 和 Mn2+ ）必须结合到这两个位点上Con A才能发挥活性。乙酰化、琥珀酰化或其他衍生物也能产生稳定的二聚体结构形式。

刀豆蛋白A（Concanavalin A，Con A）无血型特异性，但选择性结合发现于各种糖蛋白、糖脂和糖类分子上的α-甘露糖基（α-mannosyl residues）和α-葡糖基残基（α-glucosyl residues），需要Ca2+ 和 Mn2+ 离子存在才能显示活性。Con A适用于碳水化合物研究，糖蛋白纯化，酶标记（enzyme tagging），细胞膜研究，细胞凝集，和细胞分型研究。

本品是FITC标记的刀豆蛋白（FITC labeled Concanavalin A, FITC-ConA），Ex/Em=495/515nm，亲和纯化所得，基本不含未标记的荧光素。建议工作浓度范围是5-20μg/ml。

产品特性

1）   英文同义名：Concanavalin A from Canavalia ensiformis (Jack bean), FITC Conjugate; FITC-ConA;

FITC labeled Concanavalin A (ConA); ConA lectin (FITC);

2）   中文同义名：刀豆蛋白 A 来源于洋刀豆 （刀豆），FITC标记；FITC标记的刀豆凝集素A；FITC标记的刀豆球蛋白A；FITC标记的伴刀豆球蛋白A；

3）   糖类特异性：α-甘露糖；α-葡萄糖；

4）   F/P（摩尔）：2.0-6.0

5）   外观：溶液（溶于10 mM HEPES, 0.15 M NaCl, pH 7.5, containing 0.1 mM Ca2+, 0.08% sodium azide and 0.01mM Mn2+）

6）   蛋白浓度：5mg/ml

7）   Ex/Em：495/515nm

8）   应用：IF、糖生物学

保存与运输方法

保存：2-8℃避光保存，至少1年有效。

运输：冰袋运输。

注意事项

1）   本品置于2-8℃长期保存可能会产生沉淀，使用前请37℃温育数分钟，之后离心吸取上清使用。此操作基本不会对产生性能造成负影响。

2）   为了您的安全和健康，请穿实验服并戴一次性手套操作。 

常见问题

1）   FITC标记刀豆蛋白（FITC-ConA）（# MP6321-5MG）的建议工作浓度是多少？ 如何配制染色工作液？

回复：本品用于免疫荧光染色（IF）的建议工作浓度是5-20μg/ml，用户可根据自身的实际需求或参考文献来进行工作浓度的优化。可用生理缓冲液如PBS，HEPES等稀释到工作浓度。

2）   FITC标记刀豆蛋白（FITC-ConA）（# MP6321-5MG）进行免疫荧光染色（IF）的建议孵育条件如何？

回复：一般孵育条件是室温（RT）孵育30min，也可于4℃孵育30-1h。具体的孵育条件请根据自身的实际需求或参考文献来进行优化。

相关文献a）The Candida albicans ENO1 gene encodes a transglutaminase involved in growth, cell division, morphogenesis, and osmotic protection. PMID: 29386353. 【Cells were washed twice with 1× PBS, permeabilized with 70% ethanol, incubated with 2 μg/ml FITC-ConA in 1× PBS for 30 min at room temperature, washed twice with 1× PBS, and analyzed in the flow cytometer. The maximum excitation wavelength was 495 nm, and the maximum emission wavelength was 515 nm.】

相关文献b）Staining of the inner acrosomal membrane of human spermatozoa with concanavalin A lectin as an indicator of potential egg penetration ability. PMID: 1718787. 【Spermatozoa were pelleted by microcentrifugation, and excess fixative was removed. The pellet was resuspended in a small volume of fixative remaining and 20μl aliquots of fixed sperm suspension were allowed to air dry on poly-L-lysine-treated slides. The slides were then rinsed in 0.2 M glycine followed by a second wash in 10 mM phosphate buffered saline (PBS). Spermatozoa were labeled with 25μl of 100μg FITC-ConA/mL in PBS for 25 minutes at RT. Slides were rinsed with excess PBS and mounted in medium16 before immediate examination at a magnification of 400X under an Olympus BHT microscope.】

3）   FITC标记刀豆蛋白（FITC-ConA）（# MP6321-5MG）如何进行视网膜血管染色？

回复：很多临床前实验表明在各种FITC标记的凝集素中刀豆蛋白A对视网膜血管（retinal vasculature）能表现出最均匀的染色结果。很多文献用FITC-ConA来标记血管壁上粘附的白细胞和血管内皮细胞。使用方法可参考以下文献：

文献a）Simvastatin Inhibits Leukocyte Accumulation and Vascular Permeability in the Retinas of Rats with Streptozotocin-Induced Diabetes. PMID: 15111316.【Six different rats were used in each group. After the induction of deep anesthesia, the chest cavity was carefully opened and a 20-gauge perfusion cannula was introduced into the aorta. Drainage was achieved by opening the right atrium. The animals were then perfused with 100 ml of phosphate-buffered saline (PBS) to wash out blood cells in the vessels, such as erythrocytes and nonadherent leukocytes. After PBS perfusion, the animals were perfused with 25 ml of fluorescein isothiocyanate (FITC)-labeled concanavalin A lectin (ConA; 40 μg/ml in PBS, pH 7.4). ConA was used to label leukocytes adherent to the vessel walls. Residual unbound ConA was removed with PBS perfusion. The retina was carefully removed and fixed with 1% paraformaldehyde, and flat mounts were prepared using a fluorescence anti-fading medium. The retinas were then observed using fluorescence microscopy】

文献b）VEGF164 Is Proinflammatory in the Diabetic Retina. PMID: 12714656 【Perfusion with FITC-ConA or Rhodamine-Con A (40 μg/mL in PBS [pH 7.4], 5 mg/kg BW) was then performed to label adherent leukocytes and vascular endothelial cells, followed by removal of residual unbound lectin with PBS perfusion.】

 — —Written/Edited by V. Shallan【版权归MKBio懋康所有】

上海懋康生物科技有限公司是一家涉足于生命科学和生物技术领域研究的试剂、仪器和实验室消耗品与实验服务工作，主要从事细胞生物学、植物学、分子生物学、免疫学、生物化学、蛋白组学。生物制药与诊断试剂研发生产等领域。 本公司秉承“以人为本，以诚为信、合同守信”的经营理念。坚持"品质保障"的原则为广大客户提供优质产品。