Biotinylated Lycopersicon Esculentum (Tomato) Lectin (LEL, TL)

生物素化番茄凝集素

产品标签

番茄凝集素（Tomato lectin）；FITC-LEL；Intravascular perfusion 血管内灌注；Lectin perfusion 凝集素灌注；Microvasculature 微血管；Endothelial cells 内皮细胞；

产品信息【原装正品，现货供应，大量引用文献支持】

【温馨提示】：见我司（懋康生物）整理的Vector Labs血管功能研究-番茄凝集素产品专题。

产品描述

番茄凝集素（Tomato lectin，Lycopersicon Esculentum Lectin（LEL）），是一种非常稳定的单亚基糖蛋白，约含有等量的蛋白质和碳水化合物。据报道其糖类部分由85%阿拉伯糖和15%半乳糖组成，在溶液中可能形成多聚体聚合物。番茄凝集素虽然和土豆凝集素、曼陀罗凝集素（DSL）和麦胚凝集素（WGA）有部分相同的特异性，但在许多多面呈现出独特之处。番茄凝集素LEL能够很好地与血型糖蛋白（glycophorin）和Tamm-Horsfall糖蛋白结合，能够有效结合和标记啮齿类动物的血管内皮（vascular endothelium）。

本品为生物素化的番茄凝集素（来源于Lycopersicon esculentum），是一种理想的中间体检测糖偶联物，通过生物素-亲和素/链霉亲和素系统。首先加入生物素化凝集素，随后使用VECTASTAIN® ABC，Avidin D conjugate或streptavidin derivative。

产品特征

使用方法（Histochemistry）【更多请点击此处下载Lectin Immunohistochemistry, Elisa, Western Blots Guidelines】

1a. Staining procedure for paraffin sections:  Deparaffinize and hydrate tissue sections through xylenes or other clearing agents and graded alcohol series and rinse for 5 minutes in tap water.  If required, retrieve antigens using the Antigen Unmasking Solution (H-3300 or H-3301).

1b. Staining procedure for frozen sections:  Air dry sections.  Immediately before staining, fix sections with acetone.  Transfer slices to buffer.  If endogenous enzyme activities are present, inactivate using appropriate methods.

2. Perform Streptavidin/Biotin blocking if required following kit instructions (SP-2002).  Avidin in the Avidin/Biotin Blocking Kit (SP-2001) is a glycoprotein containing terminal mannose residues.  Use of the Avidin/Biotin Blocking Kit is not recommended especially when using mannose-specific lectins.

3. Block non-specific binding by incubating section with Carbo-Free™ Blocking Solution (SP-5040) for  30 minutes at room temperature.  Blot excess blocking solution from the sections.

4. Apply biotinylated lectin at approximately 2-20 µg/ml in PBS (10 mM sodium phosphate, 150 mM NaCl, pH 7.4) to the sections and incubate for 30 minutes at room temperature.  Wash with TPBS  (PBS + 0.05% Tween™ 20).

5. Prepare VECTASTAIN® ® ABC (peroxidase, PK-6100) or VECTASTAIN® ABC-AP (alkaline phosphatase, AK-5000) reagents according to the kit instructions.  Apply to the sections and incubate for 30 minutes at room temperature.  Wash with TPBS.

6. Apply an appropriate precipitating substrate for the enzyme system used in step 4.  For peroxidase, ImmPACT™ DAB (SK-4105) is recommended; for alkaline phosphatase, ImmPACT™ Vector® Red  (SK-5105).  Rinse in tap water.

7. Counterstain (optional), clear and mount.  For galactose or GalNAc-specific lectins we recommend that sections be mounted in VECTASHIELD® HardFSet™ Mounting Medium (H-1400) or VECTASHIELD® HardFSet™ Mounting Medium with DAPI (H-1500).

相关产品：

引用文献【Vector Labs，Tomato Lectin】

Huang J et al. Regression of established tumors and metastases by potent vascular endothelial growth factor blockade. Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7785-90. PMID: 12805568【Vascular lectin perfusion, 100 μg in 100 μl of saline, fluorescein- labeled Lycopersicon esculentum lectin, Vector Lab】

Inai T et al. Inhibition of vascular endothelial growth factor (VEGF) signaling in cancer causes loss of endothelial fenestrations, regression of tumor vessels, and appearance of basement membrane ghosts. Am J Pathol. 2004 Jul;165(1):35-52. PMID: 15215160【Vascular lectin perfusion, 100 μg in 100 μl of 0.9% NaCl, fluorescein- labeled Lycopersicon esculentum lectin, Vector Lab】

Thurston, G., et al., Cationic liposomes target angiogenic endothelial cells in tumors and chronic inflammation in mice. J. Clin Invest, 1998. 101 (7): p. 1401-1413. PMID: 9525983

Hashizume, H., et al., Openings between defective endothelial cells explain tumor vessel leakiness. Amer J Pathol, 2000. 156(4): p. 1363-1380. PMID: 10751361.【Vascular perfusion, biotin- or fluorescein isothiocyanate (FITC)-labeled Lycopersicon esculentum lectin, 100 μg in 100 μl 0.9% NaCl, Vector Laboratories】   

Debbage, P.L., et al., Intravital lectin perfusion analysis of vascular permeability in human micro- and macro- blood vessels. Histochemistry Cell Biol, 2001. 116(4): p. 349-359.   

Lee, J.C., et al., Interleukin-12 inhibits angiogenesis and growth of transplanted but not in situ mouse mammary tumor virus-induced mammary carcinomas. Cancer Res, 2002. 62(3): p. 747-755.   

Akerman, M.E., et al., Nanocrystal targeting in vivo. Proc Nat Acad Sci Usa, 2002. 99(20): p. 12617-12621.   

Gee, M.S., et al., Tumor vessel development and maturation impose limits on the effectiveness of anti-vascular therapy. Amer J Pathol, 2003. 162(1): p. 183-193.   

Jilani, S.M., et al., Selective binding of lectins to embryonic chicken vasculature. J Histochem Cytochem, 2003. 51(5): p. 597-604.   

Krasnici, S., et al., Effect of the surface charge of liposomes on their uptake by angiogenic tumor vessels. Int J Cancer, 2003. 105(4): p. 561-567.

 — —Written/Edited by V. Shallan【版权归MKBio懋康所有】

上海懋康生物科技有限公司是一家涉足于生命科学和生物技术领域研究的试剂、仪器和实验室消耗品与实验服务工作，主要从事细胞生物学、植物学、分子生物学、免疫学、生物化学、蛋白组学。生物制药与诊断试剂研发生产等领域。 本公司秉承“以人为本，以诚为信、合同守信”的经营理念。坚持"品质保障"的原则为广大客户提供优质产品。