质量

溶剂体积

浓度

1mg

5mg

10mg

1mM

2.3341 mL

11.6705 mL

23.3410 mL

5mM

0.4668 mL

2.3341 mL

4.6682 mL

10mM

0.2334 mL

1.1671 mL

2.3341 mL

文献1，Ng SS et al. Wortmannin inhibits pkb/akt phosphorylation and promotes gemcitabine antitumor activity in orthotopic human pancreatic cancer xenografts in immunodeficient mice. Clin Cancer Res. 2001 Oct;7(10):3269-75. MKBio PMID: 11595724

体内研究（动物模型）：

动物模型（Animal Model）：Tumor-bearing SCID mice (both s.c. and orthotopically implanted with human pancreatic adenocarcinoma cells PK1)

药物配制（Preparation）：Wortmannin was dissolved at 0.4 mg/ml in DMSO, and diluted with 0.9% NaCl before use.

实验方法（Assay）：A) In the first set of experiments, groups of tumor-bearing SCID mice (n = 4 each) were given single bolus injections of 0.7 mg/kg wortmannin via the tail vein and killed at various time points. B)  In a second series of experiments, groups of animals (n = 4 each) were injected i.v. with increasing doses of wortmannin (0.175, 0.35, and 0.7 mg/kg) and killed at 4 h. C) In a third series of experiments, tumor-bearing SCID mice were randomly assigned to four groups (n = 4 each). Each group was given i.v. bolus injections of either the drug vehicle (≤1% DMSO), 80 mg/kg of gemcitabine for 48 h, 0.7 mg/kg of wortmannin for 4 h, or 80 mg/kg of gemcitabine for 48 h and then 0.7 mg/kg wortmannin for 4 h.

文献2，Blommaart EF et al. The phosphatidylinositol 3-kinase inhibitors wortmannin and LY294002 inhibit autophagy in isolated rat hepatocytes. MKBio Eur J Biochem. 1997 Jan 15;243(1-2):240-6. PMID: 9030745

体外研究（细胞实验）：

动物模型（Animal Model）：Hepatocytes from Wistar rats

药物配制（Preparation）：Wortmannin was dissolved in DMSO at 2mM, and stored at -20℃ in the dark. Dilutions in 0.9% NaCl were prepared just before the start of each experiment. The final DMSO concentration in the incubation medium did not exceed 0.25 % (by vol.). This concentration of DMSO did not affect the processes that were tested.

实验方法（Assay）：Electron micrographs of isolated rat hepatocytes that were incubated for 60 min (5 mg dry cells/ml) with 50μM vinblastin alone (A, B) or in the presence of 50μM vinblastin plus 100 nM wortmannin.

结果（Results）：Autophagosomes could hardly be detected in cells that were incubated in the presence of 100 nM wortmannin (Fig. 3 C) compared to cells incubated under maximal autophagic conditions (Fig. 3 A and B).

货号

名称

规格

MZ0001-10MG

Wortmannin (Powder) 渥曼青霉素

10mg

MZ0002-10MG

3-Methyladenine (3-MA) 3-甲基腺嘌呤

10mg

MZ0003-10MG

LY294002

10mg

MZ0201-10MG

Rapamycin (Sirolimus) 雷帕霉素（西罗莫司）

10mg