Polyethylenimine，Linear，Mw 25，000 线性化聚乙烯亚胺（PEI 25，000）

基本描述：

线性化聚乙烯亚胺PEI 25，000，一种高电荷阳离子聚合物，非常容易结合于高电荷阴离子基质。工业应用上，线性化PEI可改善负电荷染料的物理外观，以调整染料的化学特性和增强染料与固相基质的黏附能力，常用作黏附增强剂，作用同多聚赖氨酸（poly-lysine）；科研应用上，线性化PEI能与DNA或其他负电荷生物大分子简单结合，正是这一特性，使得成为一种非常行之有效的载体运输介质（Vector carrier），即转染试剂。 

Polyethylenimine, Linear, MW 25000（PEI 25K，PEI 25，000），是一种强大的，值得信赖且非常有效的瞬时转染试剂。在HEK293和CHO表达系统，在各种生产规模（从96孔板到100L生物反应器），PEI都提供连续性的高基因表达水平。每年都有更多的研究者和公司使用Polysciences PEI在关键工作中获得优势。与大多数其他选择相比，使用PEI配制转染试剂在整个转染实验降低多达40%的开销。

化学性质：

订购信息：【原装进口，现货供应】

使用方法【来源于：CSH protocols，仅作交流学习】：

1. Pour ~450 mL of Milli-Q H2O into a 500-mL glass beaker.

2. Add 500 mg of PEI to the beaker with stirring.

3. Add concentrated HCl drop-wise to the solution to pH <2.0.

4. Stir until the PEI is dissolved (~2-3 h). Maintain the pH <2.0 throughout.

Approximately 800 μL of 12 M HCl will be required for full PEI dissolution. There may still be some small fiber particles that will not dissolve.

5. Add concentrated NaOH drop-wise to the solution to pH 7.0.

Approximately 500 μL of 10 M NaOH will be required to neutralize the PEI solution.

6. Pour the solution into a 500-mL glass cylinder. Adjust the final volume to 500 mL with Milli-Q H2O.

7. Filter-sterilize the solution through a 0.22-μm membrane.

8. Store aliquots of the desired volume at -20°C.

应用文献【基因转染用途】：

Zhong Z, Feijen J, Lok MC, Hennink WE, Christensen LV, Yockman JW, Kim YH, Kim SW. (2005). Low molecular weight linear polyethylenimine-b-poly(ethylene glycol)-b-polyethylenimine triblock copolymers: synthesis, characterization, and in vitro gene transfer properties.Biomacromolecules. 6(6):3440-8.

Sharon E. Reed, Elizabeth M. Staley, John P. Mayginnes, David J. Pintel(2006). Transfection of mammalian cells using linear polyethylenimine is a simple and effective means of producing recombinant adeno-associated virus vectors.Journal of Virological Methods.138(1–2): 85–98.

Patti A. Longo, Jennifer M. Kavran, Min-Sung Kim, and Daniel J. Leahy（2013）Transient Mammalian Cell Transfection with Polyethylenimine (PEI).Methods Enzymol. 529: 227–240.

Thomas M, Lu JJ, Ge Q, Zhang C, Chen J, Klibanov AM. (2005). Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung. Proc Natl Acad Sci U S A. 102(16):5679-84.

Durocher, Y., Perret, S. & Kamen, A. High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells. Nucleic acids research 30, E9 (2002). doi:10.1093/nar/30.2.e9

Wulhfard, S., Baldi, L., Hacker, D. L. & Wurm, F. Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells. Journal of Biotechnology 148, 128–132 (2010). doi:10.1016/j.jbiotec.2010.05.003

Baranyi, L. et al. Rapid Generation of Stable Cell Lines Expressing High Levels of Erythropoietin, Factor VIII, and an Antihuman CD20 Antibody Using Lentiviral Vectors. Human Gene Therapy Methods 24, 214–227 (2013). doi:10.1089/hgtb.2013.002

Siegman S, Truong NF, Segura T. (2015). Encapsulation of PEGylated low-molecular-weight PEI polyplexes in hyaluronic acid hydrogels reduces aggregation. Acta Biomater. in Press, doi:10.1016/j.actbio.2015.09.020

——Written/Edited by V. Shallan【版权归MKBio懋康所有】

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