产品简介:
Rapamycin
(Sirolimus) 雷帕霉素(西罗莫司)
产品标签
Rapamycin 雷帕霉素;Sirolimus 西罗莫司;3-MA
3-甲基腺嘌呤;自吞噬抑制剂 Autophagy inhibitor;mTOR;CAS:53123-88-9;
产品信息
产品名称
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产品编号
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CAS NO.
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规格
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价格(元)
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Rapamycin
(Sirolimus) 雷帕霉素(西罗莫司)
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MZ0201-10MG
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53123-88-9
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10mg
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248
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Rapamycin
(Sirolimus) 雷帕霉素(西罗莫司)
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MZ0201-50MG
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53123-88-9
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50mg
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480
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Rapamycin
(Sirolimus) 雷帕霉素(西罗莫司)
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MZ0201-100MG
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53123-88-9
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100mg
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680
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Rapamycin
(Sirolimus) 雷帕霉素(西罗莫司)
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MZ0201-500MG
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53123-88-9
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500mg
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2680
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Rapamycin
(Sirolimus) 雷帕霉素(西罗莫司)
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MZ0201-1000MG
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53123-88-9
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1g
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3980
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【温馨提示】:见我司的小分子化合物常见问题-懋康生物。
产品描述
雷帕霉素(Rapamycin),也称西罗莫司(Sirolimus),AY 22989,一种分离自细菌的大环内酯化合物,抗真菌剂和免疫抑制剂。雷帕霉素是一种特异性的mTOR抑制剂,IC50为~0.1nM(HEK293细胞)。与细胞内受体FKBP-12结合形成复合物,之后作用于mTOR中的FRB结构域从而抑制蛋白活性。雷帕霉素处理细胞引起p70 S6激酶的去磷酸化和功能失活(IC50= 50 pM)。还能引起4E-BP1/PHAS1去磷酸化,进而促使其结合并失活eIF4E。雷帕霉素能够阻断蛋白合成和诱导细胞周期停滞在G1期。还能诱导酵母和哺乳动物细胞的自吞噬现象。能驱使人多能干细胞(hPSC)分化成中内胚层和血液祖细胞。
产品特性
1)CAS NO:53123-88-9
2)化学名:(3S,6R,7E,9R,10R,12R,14S,15E,17E,19E,21S,23S,26R,27R,34aS)-9,10,12,13,14,21,22,23,24,25,
26,27,32,33,34,34a-Hexadecahydro-9,27-dihydroxy-3-[(1R)-2-[(1S,3R,4R)-4-hydroxy-3-methoxycyclohexyl]-1-methylethyl]-10,21-dimethoxy-6,8,12,14,20,26-hexamethyl-23,27-epoxy-3H-pyrido[2,1-c][1,4]oxaazacyclohentriacontine-1,5,11,28,29(4H,6H,31H)-pentone
3)同义名:Sirolimus, Antibiotic AY 22989, AY 22989, Cypher, RAPA, NSC-226080,Rapamune, SILA
9268A
4)分子式:C51H79NO13
5)分子量:914.18
6)纯度:≥98%
7)外观:白色至类白色固体
8)溶解性:溶于DMSO(25mg/ml),乙醇(15mg/ml),几乎不溶于水
9)化学结构图:
保存与运输方法
保存:-20ºC干燥保存,至少2年有效。
运输:室温运输。
使用方法【源自文献,仅作参考】
文献1,Iwamaru A et al. Silencing mammalian target of rapamycin
signaling by small interfering RNA enhances rapamycin-induced autophagy in
malignant glioma cells. Oncogene. 2007 Mar 22; 26(13):1840-51. PMID: 17001313
体外研究:
细胞类型(Cell type):Human malignant glioma U87-MG and A172 cells
药物配制(Preparation):Rapamycin was dissolved in DMSO to make 1mM stock solution and
stored at -20℃.
实验方法(Assay):Tumor cells were exposed to rapamycin (1, 10 or 100 nm) for 24
or 48 h. The cytotoxic effect of rapamycin was determined using a WST-1 cell
proliferation assay.
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文献2,Caramés B et al. Autophagy activation by rapamycin reduces
severity of experimental osteoarthritis. Ann Rheum Dis. 2012
Apr;71(4):575-81. PMID: 22084394
体内研究:
动物模型(Animal Model):Experimental osteoarthritis was induced in 2-month-old male
C57Bl/6J mice
药物配制(Preparation):Rapamycin was dissolved in DMSO at 25mg/ml and stored at -20℃. For injection, the stock solution was diluted in phosphate
buffered saline (PBS).
注射剂量(Dosages):Mice received daily intraperitoneal injections of rapamycin at
1 mg/kg body weight/dose in a total injection volume of 0.3 ml for 10 weeks
and control animals received the DMSO vehicle at 0.4% in a total injection
volume of 0.3 ml.
给药途径(Administration):Intraperitoneal (i.p.) injection
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文献3,Eshleman JS et al. Inhibition of the Mammalian Target of
Rapamycin Sensitizes U87 Xenografts to Fractionated Radiation Therapy. Cancer
Res. 2002 Dec 15;62(24):7291-7. PMID: 12499272
体外研究:
细胞类型(Cell type):A172, U87, and U118 malignant glioma cell lines
药物配制(Preparation):Rapamycin (NSC 226080) was dissolved in ethanol to yield a 5
mg/ml stock solution, which was stored at −20°C.
实验方法(Assay):Tumor cells were incubated with 0 or 100 nM rapamycin at 37°C for
72 h and then processed in a MTS assay.
体内研究:
动物模型(Animal Model):8-10-week-old female athymic nude mice by s.c. injection of
2-5 million U87 cells
药物配制(Preparation):For rapamycin injections, stock
rapamycin was diluted first in sterile 10% PEG400/8% ethanol and then in an
equal volume of sterile 10% Tween 80 for a final concentration of 20 μg
rapamycin/100 μl. Rapamycin was delivered by i.p. injection, and the doses of
rapamycin were calculated assuming that all mice weighed 20 g.
给药途径(Administration):Intraperitoneal (i.p.) injection
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注意事项
1)雷帕霉素几乎不溶于水(文献报道仅2.6µg/ml)。[Simamora P et al. Int J Pharm. 2001 Feb
1;213(1-2):25-9.]
2)本品不是临床药物,只能用于科研用途,不能用于诊断或临床用途。
3)为了您的安全和健康,请穿实验服并戴一次性手套操作。
相关产品
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上海懋康生物科技有限公司是一家涉足于生命科学和生物技术领域研究的试剂、仪器和实验室消耗品与实验服务工作,主要从事细胞生物学、植物学、分子生物学、免疫学、生物化学、蛋白组学。生物制药与诊断试剂研发生产等领域。 本公司秉承“以人为本,以诚为信、合同守信”的经营理念。坚持"品质保障"的原则为广大客户提供优质产品。
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